Dual Stimulus-responsive Renewable Nanoadsorbent for Selective Adsorption of Low-density Lipoprotein in Serum

Overview

Journal Regen Biomater

Specialty Biomedical Engineering

Date 2024 Jun 7

PMID 38845854

Authors

Chen Guo

Xinbang Jiang

Xiaofang Guo

Zhuang Liu

Biao Wang

Yunzheng Du

Ziying Tian

Zimeng Wang

Lailiang Ou

Affiliations

Soon will be listed here.

Abstract

Selective removal of ultra-high low-density lipoprotein (LDL) from the blood of hyperlipemia patients using hemoperfusion is considered an efficient method to prevent the deterioration of atherosclerotic cardiovascular disease. Based on the exceptional structure-function properties of multistimulus-responsive materials, we developed a magnetic photorenewable nanoadsorbent (FeO@SiO@Azo-COOH) with outstanding selectivity and regenerative characteristics, featuring functionalized azobenzene as the ligand. The dual-stimulus response endowed FeO@SiO@Azo-COOH with rapid separation and photoregenerative properties. The adsorbent demonstrated excellent removal efficiency of LDL with an adsorption capacity of 15.06 mg/g, and highly repetitive adsorption performance (≥5 cycles) under irradiation. FeO@SiO@Azo-COOH also exhibited remarkable adsorption properties and selectivity in human serum, with adsorption capacities of 10.93, 21.26 and 9.80 mg/g for LDL, total cholesterol and triglycerides and only 0.77 mg/g for high-density lipoprotein (HDL), resulting in a 93% selective adsorption difference (LDL/HDL). Complete green regeneration of the nanoadsorbent was achieved through a simple regeneration process, maintaining a recovery rate of 99.4% after five regeneration experiments. By combining dynamic perfusion experiment with micromagnetic microfluidics, the LDL content decreased by 16.6%. Due to its superior adsorption capacity and regenerative properties, the dual stimulus-responsive nanosorbent is considered a potential hemoperfusion adsorbent.

References

Ma K, Ma L, Cai S, Wang X, Liu B, Xu Z . Preparation of heparin-immobilized PVA and its adsorption for low-density lipoprotein from hyperlipemia plasma. J Mater Sci Mater Med. 2008; 19(10):3255-61. DOI: 10.1007/s10856-008-3461-y. View

Cronan J . The Escherichia coli FadR transcription factor: Too much of a good thing?. Mol Microbiol. 2020; 115(6):1080-1085. PMC: 8180525. DOI: 10.1111/mmi.14663. View

Wang Y, Sun L, Guo J, Shi K, Shang L, Xiao J . Pollens derived magnetic porous particles for adsorption of low-density lipoprotein from plasma. Bioact Mater. 2020; 6(6):1555-1562. PMC: 7691160. DOI: 10.1016/j.bioactmat.2020.11.015. View

Wang T, Sun X, Guo X, Zhang J, Yang J, Tao S . Ultraefficiently Calming Cytokine Storm Using TiCT MXene. Small Methods. 2021; 5(5):e2001108. PMC: 7995020. DOI: 10.1002/smtd.202001108. View

Guo C, Yu Y, Jiang X, Ma B, Liu Z, Chai Y . Photorenewable Azobenzene Polymer Brush-Modified Nanoadsorbent for Selective Adsorption of LDL in Serum. ACS Appl Mater Interfaces. 2022; 14(30):34388-34399. DOI: 10.1021/acsami.2c07193. View

Robinson J, Nedergaard B, Rogers W, Fialkow J, Neutel J, Ramstad D . Effect of evolocumab or ezetimibe added to moderate- or high-intensity statin therapy on LDL-C lowering in patients with hypercholesterolemia: the LAPLACE-2 randomized clinical trial. JAMA. 2014; 311(18):1870-82. DOI: 10.1001/jama.2014.4030. View

Wang W, Xie H, Sun L, Ou L, Wang L, Yu Y . Macroporous poly(vinyl alcohol) microspheres bearing phosphate groups as a new adsorbent for low-density lipoprotein apheresis. Biomed Mater. 2009; 4(6):065007. DOI: 10.1088/1748-6041/4/6/065007. View

Huang H, Yu H, Lin L, Chen J, Zhu P . Protective effect of sonic hedgehog against oxidized low‑density lipoprotein‑induced endothelial apoptosis: Involvement of NF‑κB and Bcl‑2 signaling. Int J Mol Med. 2020; 45(6):1864-1874. PMC: 7169656. DOI: 10.3892/ijmm.2020.4542. View

Kang J, Super M, Yung C, Cooper R, Domansky K, Graveline A . An extracorporeal blood-cleansing device for sepsis therapy. Nat Med. 2014; 20(10):1211-6. DOI: 10.1038/nm.3640. View

10.

Yvan-Charvet L, Kling J, Pagler T, Li H, Hubbard B, Fisher T . Cholesterol efflux potential and antiinflammatory properties of high-density lipoprotein after treatment with niacin or anacetrapib. Arterioscler Thromb Vasc Biol. 2010; 30(7):1430-8. PMC: 2917780. DOI: 10.1161/ATVBAHA.110.207142. View

11.

Huddy K, Dhesi P, Thompson P . Do the frequencies of adverse events increase, decrease, or stay the same with long-term use of statins?. Curr Atheroscler Rep. 2013; 15(2):301. DOI: 10.1007/s11883-012-0301-9. View

12.

Jiang W, Wang G, He Y, Wang X, An Y, Song Y . Photo-switched wettability on an electrostatic self-assembly azobenzene monolayer. Chem Commun (Camb). 2005; (28):3550-2. DOI: 10.1039/b504479k. View

13.

Knetsch M, Aldenhoff Y, Koole L . The effect of high-density-lipoprotein on thrombus formation on and endothelial cell attachement to biomaterial surfaces. Biomaterials. 2006; 27(14):2813-9. DOI: 10.1016/j.biomaterials.2005.12.025. View

14.

Sivaramakrishnan S, Keerthi K, Gates K . A chemical model for redox regulation of protein tyrosine phosphatase 1B (PTP1B) activity. J Am Chem Soc. 2005; 127(31):10830-1. DOI: 10.1021/ja052599e. View

15.

Yu Y, Dong J, Ma B, Jiang X, Guo C, Liu Z . Bio-inspired dual-functional phospholipid-poly(acrylic acid) brushes grafted porous poly(vinyl alcohol) beads for selective adsorption of low-density lipoprotein. J Mater Chem B. 2021; 9(32):6364-6376. DOI: 10.1039/d1tb01220g. View

16.

Thierer J, Ekker S, Farber S . The LipoGlo reporter system for sensitive and specific monitoring of atherogenic lipoproteins. Nat Commun. 2019; 10(1):3426. PMC: 6668417. DOI: 10.1038/s41467-019-11259-w. View

17.

Odabas S, Sayar F, Guven G, Yanikkaya-Demirel G, Piskin E . Separation of mesenchymal stem cells with magnetic nanosorbents carrying CD105 and CD73 antibodies in flow-through and batch systems. J Chromatogr B Analyt Technol Biomed Life Sci. 2007; 861(1):74-80. DOI: 10.1016/j.jchromb.2007.11.017. View

18.

Prassl R, Laggner P . Molecular structure of low density lipoprotein: current status and future challenges. Eur Biophys J. 2008; 38(2):145-58. DOI: 10.1007/s00249-008-0368-y. View

19.

Mundi S, Massaro M, Scoditti E, Carluccio M, van Hinsbergh V, Iruela-Arispe M . Endothelial permeability, LDL deposition, and cardiovascular risk factors-a review. Cardiovasc Res. 2017; 114(1):35-52. PMC: 7729208. DOI: 10.1093/cvr/cvx226. View

20.

Wang S, Yu Y, Cui T, Cheng Y . A novel amphiphilic adsorbent for the removal of low-density lipoprotein. Biomaterials. 2003; 24(16):2799-802. DOI: 10.1016/s0142-9612(03)00092-9. View