Multiomics Analysis of a Resistant European Turnip ECD04 During Clubroot Infection Reveals Key Hub Genes Underlying Resistance Mechanism

Overview

Journal Front Plant Sci

Date 2024 Jun 7

PMID 38845850

Authors

Xueqing Zhou

Ting Zhong

Meixiu Wu

Qian Li

Wenlin Yu

Longcai Gan

Xianyu Xiang

Yunyun Zhang

Yaru Shi

Yuanwei Zhou

Peng Chen

Chunyu Zhang

Affiliations

Soon will be listed here.

Abstract

The clubroot disease has become a worldwide threat for crucifer crop production, due to its soil-borne nature and difficulty to eradicate completely from contaminated field. In this study we used an elite resistant European fodder turnip ECD04 and investigated its resistance mechanism using transcriptome, sRNA-seq, degradome and gene editing. A total of 1751 DEGs were identified from three time points after infection, among which 7 hub genes including for cell wall assembly and two genes in PTI pathways. On microRNA, we identified 17 DEMs and predicted 15 miRNA-target pairs (DEM-DEG). We validated two pairs (miR395- and miR160-) by degradome sequencing. We investigated the miR395- pair by CRISPR-Cas9 mediated gene editing, the result showed that knocking-out could lead to elevated clubroot resistance in . In summary, the data acquired on transcriptional response and microRNA as well as target genes provide future direction especially gene candidates for genetic improvement of clubroot resistance on Brassica species.

Citing Articles

Use of CRISPR Technology in Gene Editing for Tolerance to Biotic Factors in Plants: A Systematic Review.

Mascarenhas M, Nascimento F, Rocha A, Ferreira M, Oliveira W, Lino L Curr Issues Mol Biol. 2024; 46(10):11086-11123.

PMID: 39451539 PMC: 11505962. DOI: 10.3390/cimb46100659.

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