An ELISA Test Using a Circulating Peptide for Detecting Bovine Tuberculosis in Dairy Cattle

Overview

Journal Front Vet Sci

Specialty Veterinary Medicine

Date 2024 Jun 6

PMID 38840633

Authors

Tawatchai Singhla

Sukolrat Boonyayatra

Nattawooti Sthitmatee

Anucha Sirimalaisuwan

Nitit Maicharoen

Aratchaporn Meemey

Anucha Muenthaisong

Amarin Rittipornlertrak

Srinand Sreevatsan

Affiliations

Soon will be listed here.

Abstract

This study aimed to determine the sensitivity (Se) and specificity (Sp) of a circulating pathogen-specific biomarker (polyketide synthetase 5, Pks5)-based enzyme-linked immunosorbent assay (ELISA) independently or in conjunction with a caudal fold tuberculin (CFT) test for bovine tuberculosis (bTB) screening in dairy cattle. We enrolled 987 dairy cows from 34 herds in Chiang Mai province, Thailand. A conditionally independent Bayesian model with a single population was inferred from the test results. The percentage of positive results for the Pks5-ELISA using 0.4 OD cutoff test and CFT test were 9.0% (89/987) and 10.5% (104/987), respectively. The median of posterior estimates of Se for the Pks5-ELISA test was 90.2% (95% posterior probability interval [PPI] = 76.6-97.4%), while the estimated Sp was slightly higher (median = 92.9, 95% PPI = 91.0-94.5%). The median estimated Se of the CFT test was 85.9% (95% PPI = 72.4-94.6%), while the estimated Sp was higher, with a median of 90.7% (95% PPI = 88.7-92.5%). The posterior estimate for true disease prevalence was 2.4% (95% PPI = 1.2-3.9%). The Pks5-ELISA test yielded characteristics at or above the acceptable standards for bTB detection. Therefore, the pathogen-specific biomarker, Pks5, is a potential detection system for bTB screening and may be applied as an ancillary test together with the currently applied standard method (CFT test) to reinforce the bTB control and eradication programs.

References

Echeverria G, Zumarraga M, Proano-Perez F, Blasco F, de Waard J . Assessing the impact of various tuberculin PPD brands on bovine tuberculosis diagnosis. Sci Rep. 2024; 14(1):5155. PMC: 10908831. DOI: 10.1038/s41598-024-52089-1. View

Abbate J, Arfuso F, Iaria C, Arestia G, Lanteri G . Prevalence of Bovine Tuberculosis in Slaughtered Cattle in Sicily, Southern Italy. Animals (Basel). 2020; 10(9). PMC: 7552133. DOI: 10.3390/ani10091473. View

Norby B, Bartlett P, Fitzgerald S, Granger L, Bruning-Fann C, Whipple D . The sensitivity of gross necropsy, caudal fold and comparative cervical tests for the diagnosis of bovine tuberculosis. J Vet Diagn Invest. 2004; 16(2):126-31. DOI: 10.1177/104063870401600206. View

Coad M, Clifford D, Rhodes S, Hewinson R, Vordermeier H, Whelan A . Repeat tuberculin skin testing leads to desensitisation in naturally infected tuberculous cattle which is associated with elevated interleukin-10 and decreased interleukin-1 beta responses. Vet Res. 2009; 41(2):14. PMC: 2785049. DOI: 10.1051/vetres/2009062. View

Carneiro P, de Moura Sousa E, Viana R, Monteiro B, do Socorro Lima Kzam A, de Souza D . Study on supplemental test to improve the detection of bovine tuberculosis in individual animals and herds. BMC Vet Res. 2021; 17(1):137. PMC: 8011399. DOI: 10.1186/s12917-021-02839-4. View

Alvarez J, Perez A, Bezos J, Marques S, Grau A, Saez J . Evaluation of the sensitivity and specificity of bovine tuberculosis diagnostic tests in naturally infected cattle herds using a Bayesian approach. Vet Microbiol. 2011; 155(1):38-43. DOI: 10.1016/j.vetmic.2011.07.034. View

Lamont E, Janagama H, Ribeiro-Lima J, Vulchanova L, Seth M, Yang M . Circulating Mycobacterium bovis peptides and host response proteins as biomarkers for unambiguous detection of subclinical infection. J Clin Microbiol. 2014; 52(2):536-43. PMC: 3911325. DOI: 10.1128/JCM.02433-13. View

Allen A, Skuce R, Byrne A . Bovine Tuberculosis in Britain and Ireland - A Perfect Storm? the Confluence of Potential Ecological and Epidemiological Impediments to Controlling a Chronic Infectious Disease. Front Vet Sci. 2018; 5:109. PMC: 6008655. DOI: 10.3389/fvets.2018.00109. View

Nunez-Garcia J, Downs S, Parry J, Abernethy D, Broughan J, Cameron A . Meta-analyses of the sensitivity and specificity of ante-mortem and post-mortem diagnostic tests for bovine tuberculosis in the UK and Ireland. Prev Vet Med. 2017; 153:94-107. DOI: 10.1016/j.prevetmed.2017.02.017. View

10.

Good M, Bakker D, Duignan A, Collins D . The History of Tuberculin Testing in Bovines: Tuberculosis, a "One Health" Issue. Front Vet Sci. 2018; 5:59. PMC: 5900347. DOI: 10.3389/fvets.2018.00059. View

11.

Branscum A, Gardner I, Johnson W . Estimation of diagnostic-test sensitivity and specificity through Bayesian modeling. Prev Vet Med. 2005; 68(2-4):145-63. DOI: 10.1016/j.prevetmed.2004.12.005. View

12.

Abdellrazeq G, Elnaggar M, Osman H, Davis W, Singh M . Prevalence of Bovine Tuberculosis in Egyptian Cattle and the Standardization of the Interferon-gamma Assay as an Ancillary Test. Transbound Emerg Dis. 2014; 63(5):497-507. DOI: 10.1111/tbed.12291. View

13.

Kelly R, Gonzalez Gordon L, Egbe N, Freeman E, Mazeri S, Ngwa V . Bovine Tuberculosis Antemortem Diagnostic Test Agreement and Disagreement in a Naturally Infected African Cattle Population. Front Vet Sci. 2022; 9:877534. PMC: 9301138. DOI: 10.3389/fvets.2022.877534. View

14.

Singhla T, Boonyayatra S, Chulakasian S, Lukkana M, Alvarez J, Sreevatsan S . Determination of the sensitivity and specificity of bovine tuberculosis screening tests in dairy herds in Thailand using a Bayesian approach. BMC Vet Res. 2019; 15(1):149. PMC: 6524330. DOI: 10.1186/s12917-019-1905-x. View

15.

Jang Y, Kim T, Jeong M, Seo Y, Ryoo S, Park C . Introduction and Application of the Interferon-γ Assay in the National Bovine Tuberculosis Control Program in South Korea. Front Vet Sci. 2020; 7:222. PMC: 7198753. DOI: 10.3389/fvets.2020.00222. View

16.

Borham M, Oreiby A, El-Gedawy A, Hegazy Y, Khalifa H, Al-Gaabary M . Review on Bovine Tuberculosis: An Emerging Disease Associated with Multidrug-Resistant Species. Pathogens. 2022; 11(7). PMC: 9320398. DOI: 10.3390/pathogens11070715. View

17.

Picasso-Risso C, Perez A, Gil A, Nunez A, Salaberry X, Suanes A . Modeling the Accuracy of Two Bovine Tuberculosis Tests Using a Bayesian Approach. Front Vet Sci. 2019; 6:261. PMC: 6701407. DOI: 10.3389/fvets.2019.00261. View

18.

Bezos J, Saez-Llorente J, Alvarez J, Romero B, Diez-Guerrier A, Dominguez L . Bovine tuberculosis in Spain, is it really the final countdown?. Ir Vet J. 2023; 76(Suppl 1):13. PMC: 10369685. DOI: 10.1186/s13620-023-00241-0. View

19.

Singhla T, Pikulkaew S, Boonyayatra S . Performance of Loop-Mediated Isothermal Amplification Technique in Milk Samples for the Diagnosis of Bovine Tuberculosis in Dairy Cattle Using a Bayesian Approach. Pathogens. 2022; 11(5). PMC: 9144818. DOI: 10.3390/pathogens11050573. View

20.

Li T, Liu P . Comparison of Two Bayesian Methods in Evaluation of the Absence of the Gold Standard Diagnostic Tests. Biomed Res Int. 2019; 2019:1374748. PMC: 6720053. DOI: 10.1155/2019/1374748. View