Effect of Dexamethasone on Serum Protein Extravasation in Experimental Brain Infarcts of Monkey: an Immunohistochemical Study

Overview

Journal Acta Neuropathol

Specialty Neurology

Date 1985 Jan 1

PMID 3883688

Citations 5

Authors

L M Barbosa-Coutinho

A Hartmann

K A Hossmann

T Rommel

Affiliations

Soon will be listed here.

Abstract

Experimental brain infarcts were produced in 12 adult baboons (Papio cynocephalus) by transorbital permanent clipping of the left middle cerebral artery. One group (seven monkeys) received daily injections of 1 mg/kg dexamethasone, starting 1 h after vascular occlusion and continuing till the end of the experiment. Another group (five monkeys) was not treated. One week after vascular occlusion the volume of infarcts and peri-infarct edema was estimated morphometrically on histological sections, using Masson's trichrome stain and the peroxidase-anti-peroxidase (PAP) technique for visualization of serum protein extravasation. In the untreated animals the average volume of infarct was 6.57 +/- 4.23% (mean +/- SD) and the volume of edema 7.83 +/- 2.93% of ipsilateral hemisphere. In the treated animals the infarct volume was not different (7.95 +/- 3.00%), but the volume of peri-infarct edema was significantly lower (2.82 +/- 3.06%, p less than 0.05). The results obtained indicate that dexamethasone treatment reduces the development of peri-infarct edema but does not influence the size of infarcts.

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References

Little J . Modification of acute focal ischemia by treatment with mannitol and high-dose dexamethasone. J Neurosurg. 1978; 49(4):517-24. DOI: 10.3171/jns.1978.49.4.0517. View

Hartmann A, Menzel J, Buttinger C, Lange D, Alberti E . [Regional cerebral blood flow in the baboon during ischaemic cerebral infarction under dexamethasone treatment (author's transl)]. Fortschr Neurol Psychiatr. 1981; 49(10):380-92. DOI: 10.1055/s-2007-1002342. View

Theodore D, Abraham J . A preliminary report on tissue pressure in ischaemic primate brain. Indian J Med Res. 1981; 74:102-6. View

Ng L, Nimmannitya J . Massive cerebral infarction with severe brain swelling: a clinicopathological study. Stroke. 1970; 1(3):158-63. DOI: 10.1161/01.str.1.3.158. View

Norris J . Steroid therapy in acute cerebral infarction. Arch Neurol. 1976; 33(1):69-71. DOI: 10.1001/archneur.1976.00500010071014. View

Hossmann K, Hurter T, Oschlies U . The effect of dexamethasone on serum protein extravasation and edema development in experimental brain tumors of cat. Acta Neuropathol. 1983; 60(3-4):223-31. DOI: 10.1007/BF00691870. View

Symon L, Ishikawa S, Meyer J . Cerebral arterial pressure changes and development of leptomeningeal collateral circulation. Neurology. 1963; 13:237-50. DOI: 10.1212/wnl.13.3.237. View

Kamijyo Y, Garcia J, Cooper J . Temporary regional cerebral ischemia in the cat. A model of hemorrhagic and subcortical infarction. J Neuropathol Exp Neurol. 1977; 36(2):338-50. DOI: 10.1097/00005072-197703000-00008. View

Matsuoka Y, Hossmann K . Corticosteroid therapy of experimental tumour oedema. Neurosurg Rev. 1981; 4(4):185-90. DOI: 10.1007/BF01743708. View

10.

Schuier F, Hossmann K . Experimental brain infarcts in cats. II. Ischemic brain edema. Stroke. 1980; 11(6):593-601. DOI: 10.1161/01.str.11.6.593. View

11.

Hudgins W, Garcia J . Transorbital approach to the middle cerebral artery of the squirrel monkey: a technique for experimental cerebral infarction applicable to ultrastructural studies. Stroke. 1970; 1(2):107-11. DOI: 10.1161/01.str.1.2.107. View

12.

ZULCH K . Edema in cerebrovascular disease. Bibl Anat. 1977; (16 Pt 2):238-41. View

13.

Date H, Hossmann K . Effect of vasodilating drugs on intracortical and extracortical vascular resistance following middle cerebral artery occlusion in cats. Ann Neurol. 1984; 16(3):330-6. DOI: 10.1002/ana.410160309. View

14.

Yamada K, Bremer A, West C . Effects of dexamethasone on tumor-induced brain edema and its distribution in the brain of monkeys. J Neurosurg. 1979; 50(3):361-7. DOI: 10.3171/jns.1979.50.3.0361. View

15.

PAPPIUS H, Wolfe L . Functional disturbances in brain following injury: search for underlying mechanisms. Neurochem Res. 1983; 8(1):63-72. DOI: 10.1007/BF00965654. View

16.

CROWELL R, Olsson Y, KLATZO I, Ommaya A . Temporary occlusion of the middle cerebral artery in the monkey: clinical and pathological observations. Stroke. 1970; 1(6):439-48. DOI: 10.1161/01.str.1.6.439. View

17.

Anderson D, Cranford R . Corticosteroids in ischemic stroke. Stroke. 1979; 10(1):68-71. DOI: 10.1161/01.str.10.1.68. View

18.

Fenske A, Fischer M, Regli F, Hase U . The response of focal ischemic cerebral edema to dexamethasone. J Neurol. 1979; 220(3):199-209. DOI: 10.1007/BF00705537. View

19.

PAPPIUS H . Dexamethasone and local cerebral glucose utilization in freeze-traumatized rat brain. Ann Neurol. 1982; 12(2):157-62. DOI: 10.1002/ana.410120205. View

20.

Suzuki O, Takanohashi M, Yagi K . Protective effect of dexamethasone on enhancement of blood-brain barrier permeability caused by electroconvulsive shock. Arzneimittelforschung. 1976; 26(4):533-4. View