MiR-483-3p Promotes Dental Pulp Stem Cells Osteogenic Differentiation Via the MAPK Signaling Pathway by Targeting ARRB2

Overview

Journal In Vitro Cell Dev Biol Anim

Publisher Springer

Specialties Biology
Cell Biology

Date 2024 Jun 4

PMID 38833209

Authors

Xin Yu

Juan Ge

Huimin Xie

Jialu Qian

Wenqian Xia

Qinghua Wang

Xiaorong Zhou

Yan Zhou

Affiliations

Soon will be listed here.

Abstract

Human dental pulp stem cells (DPSCs) have become an important component for bone tissue engineering and regenerative medicine due to their ability to differentiate into osteoblast precursors. Two miRNA chip datasets (GSE138180 and E-MTAB-3077) of DPSCs osteogenic differentiation were analyzed respectively to find the expression of miR-483-3p significantly increased in the differentiated groups. We further confirmed that miR-483-3p continued to overexpress during osteogenic differentiation of DPSCs, especially reaching its peak on the 7th day. Moreover, miR-483-3p could significantly promote the expression of osteogenic markers including RUNX2 and OSX, and activate MAPK signaling pathway by inducing phosphorylation of ERK, p38, and JNK. In addition, as a significant gene within the MAPK signaling pathway, ARRB2 was identified as the target gene of miR-483-3p by bioinformatic prediction and experimental verification. In conclusion, we identified miR-483-3p could promote osteogenic differentiation of DPSCs via the MAPK signaling pathway by targeting ARRB2.

References

Agarwal V, Bell G, Nam J, Bartel D . Predicting effective microRNA target sites in mammalian mRNAs. Elife. 2015; 4. PMC: 4532895. DOI: 10.7554/eLife.05005. View

Bae W, Choi J, Nam S, Jeong J . β-arrestin 2 stimulates degradation of HIF-1α and modulates tumor progression of glioblastoma. Cell Death Differ. 2021; 28(11):3092-3104. PMC: 8563934. DOI: 10.1038/s41418-021-00802-2. View

Chen Y, Wang X . miRDB: an online database for prediction of functional microRNA targets. Nucleic Acids Res. 2019; 48(D1):D127-D131. PMC: 6943051. DOI: 10.1093/nar/gkz757. View

Chen Z, Zhang K, Qiu W, Luo Y, Pan Y, Li J . Genome-wide identification of long noncoding RNAs and their competing endogenous RNA networks involved in the odontogenic differentiation of human dental pulp stem cells. Stem Cell Res Ther. 2020; 11(1):114. PMC: 7071724. DOI: 10.1186/s13287-020-01622-w. View

Choi B, Kim J, Park S, Kim E, Oh S, Choi H . Sphingosine-1-phosphate hinders the osteogenic differentiation of dental pulp stem cells in association with AKT signaling pathways. Int J Oral Sci. 2022; 14(1):21. PMC: 9033766. DOI: 10.1038/s41368-022-00173-5. View

DAquino R, De Rosa A, Lanza V, Tirino V, Laino L, Graziano A . Human mandible bone defect repair by the grafting of dental pulp stem/progenitor cells and collagen sponge biocomplexes. Eur Cell Mater. 2009; 18:75-83. DOI: 10.22203/ecm.v018a07. View

Edgar R, Domrachev M, Lash A . Gene Expression Omnibus: NCBI gene expression and hybridization array data repository. Nucleic Acids Res. 2001; 30(1):207-10. PMC: 99122. DOI: 10.1093/nar/30.1.207. View

Gronthos S, Mankani M, Brahim J, Robey P, Shi S . Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci U S A. 2000; 97(25):13625-30. PMC: 17626. DOI: 10.1073/pnas.240309797. View

Han B, Yang X, Zhang P, Zhang Y, Tu Y, He Z . DNA methylation biomarkers for nasopharyngeal carcinoma. PLoS One. 2020; 15(4):e0230524. PMC: 7144954. DOI: 10.1371/journal.pone.0230524. View

10.

Han Y, Li X, Zhang Y, Han Y, Chang F, Ding J . Mesenchymal Stem Cells for Regenerative Medicine. Cells. 2019; 8(8). PMC: 6721852. DOI: 10.3390/cells8080886. View

11.

Huang H, Lin Y, Cui S, Huang Y, Tang Y, Xu J . miRTarBase update 2022: an informative resource for experimentally validated miRNA-target interactions. Nucleic Acids Res. 2021; 50(D1):D222-D230. PMC: 8728135. DOI: 10.1093/nar/gkab1079. View

12.

Iaquinta M, Lanzillotti C, Mazziotta C, Bononi I, Frontini F, Mazzoni E . The role of microRNAs in the osteogenic and chondrogenic differentiation of mesenchymal stem cells and bone pathologies. Theranostics. 2021; 11(13):6573-6591. PMC: 8120225. DOI: 10.7150/thno.55664. View

13.

Jiang Z, Hua Y . Hydrogen sulfide promotes osteogenic differentiation of human periodontal ligament cells via p38-MAPK signaling pathway under proper tension stimulation. Arch Oral Biol. 2016; 72:8-13. DOI: 10.1016/j.archoralbio.2016.08.008. View

14.

Kallifatidis G, Smith D, Morera D, Gao J, Hennig M, Hoy J . β-Arrestins Regulate Stem Cell-Like Phenotype and Response to Chemotherapy in Bladder Cancer. Mol Cancer Ther. 2019; 18(4):801-811. PMC: 6510267. DOI: 10.1158/1535-7163.MCT-18-1167. View

15.

Karavidha K, Burmeister M, Greenwald M . β-Arrestin 2 (ARRB2) Polymorphism is Associated With Adverse Consequences of Chronic Heroin Use. Am J Addict. 2021; 30(4):351-357. PMC: 8243774. DOI: 10.1111/ajad.13150. View

16.

Kuchler E, Lips A, Tannure P, Ho B, Costa M, Granjeiro J . Tooth agenesis association with self-reported family history of cancer. J Dent Res. 2012; 92(2):149-55. DOI: 10.1177/0022034512468750. View

17.

Laino G, DAquino R, Graziano A, Lanza V, Carinci F, Naro F . A new population of human adult dental pulp stem cells: a useful source of living autologous fibrous bone tissue (LAB). J Bone Miner Res. 2005; 20(8):1394-402. DOI: 10.1359/JBMR.050325. View

18.

Liang H, Lin Z, Ye Y, Luo R, Zeng L . ARRB2 promotes colorectal cancer growth through triggering WTAP. Acta Biochim Biophys Sin (Shanghai). 2020; 53(1):85-93. DOI: 10.1093/abbs/gmaa151. View

19.

Luo G, Wang X, Liu C . MiR-483-3p improves learning and memory abilities via XPO1 in Alzheimer's disease. Brain Behav. 2022; 12(8):e2680. PMC: 9392541. DOI: 10.1002/brb3.2680. View

20.

Maemura T, Fukuyama S, Sugita Y, Lopes T, Nakao T, Noda T . Lung-Derived Exosomal miR-483-3p Regulates the Innate Immune Response to Influenza Virus Infection. J Infect Dis. 2018; 217(9):1372-1382. DOI: 10.1093/infdis/jiy035. View