The Possible Protective Role of HLA B27 and Relevant Immune Markers in Juvenile Idiopathic Arthritis Patients

Overview

Journal Pak J Med Sci

Specialty General Medicine

Date 2024 Jun 3

PMID 38827853

Authors

Farhana Shahzad

Romeeza Tahir

Faheem Shahzad

Nadeem Afzal

Affiliations

Soon will be listed here.

Abstract

Background & Objectives: JIA is a disease with different immunological characteristics and a complicated genetic foundation. HLA B27 is a risk factor for the development of JIA, and its impact on immunopathogenesis of the disease is also an area of interest. To determine whether HLA B27 and immune markers varied between JIA patients and healthy population.

Methods: This comparative cross-sectional study was conducted at Immunology Department of University of Health sciences (UHS), Lahore from February 2018 till August 2021. A total of (71) JIA patients and (34) healthy controls were enrolled. B cells were enumerated by flowcytometry, ELISA was used for serum cytokines estimation and HLA B27 allele was detected by SPSS polymerase chain reaction.

Results: The HLA B27 allele was significantly more in the control group than in the patient group, suggesting it is a protective allele to prevent JIA. Peripheral blood B cell counts and percentages were significantly lower in the HLA B27 positive group than in the HLA B27 negative group of control population. Serum cytokine levels were not significantly different between the HLA B27 positive and HLA B27 negative allele of the two study populations.

Conclusion: In this study B cells are different between the two groups of control population however; serum cytokines are comparable between the study groups. Though, it was indicated that HLA B27 may be a preventive allele in the onset of JIA.

References

Ambarus C, Yeremenko N, Baeten D . Altered cytokine expression by macrophages from HLA-B27-positive spondyloarthritis patients without evidence of endoplasmic reticulum stress. Rheumatol Adv Pract. 2019; 2(1):rky014. PMC: 6649909. DOI: 10.1093/rap/rky014. View

DeLay M, Turner M, Klenk E, Smith J, Sowders D, Colbert R . HLA-B27 misfolding and the unfolded protein response augment interleukin-23 production and are associated with Th17 activation in transgenic rats. Arthritis Rheum. 2009; 60(9):2633-43. PMC: 2893331. DOI: 10.1002/art.24763. View

Christiansen F, Hawkins B, Dawkins R . Immune function in ankylosing spondylitics and their relatives: influence of disease and HLA B27. Clin Exp Immunol. 1978; 33(2):270-5. PMC: 1537569. View

Akioka S . Interleukin-6 in juvenile idiopathic arthritis. Mod Rheumatol. 2019; 29(2):275-286. DOI: 10.1080/14397595.2019.1574697. View

Piper C, Pesenacker A, Bending D, Thirugnanabalan B, Varsani H, Wedderburn L . T cell expression of granulocyte-macrophage colony-stimulating factor in juvenile arthritis is contingent upon Th17 plasticity. Arthritis Rheumatol. 2014; 66(7):1955-60. PMC: 4190686. DOI: 10.1002/art.38647. View

Mohyuddin A, Ayub Q, Khaliq S, Mansoor A, Mazhar K, Rehman S . HLA polymorphism in six ethnic groups from Pakistan. Tissue Antigens. 2002; 59(6):492-501. DOI: 10.1034/j.1399-0039.2002.590606.x. View

Naz S, Mushtaq A, Rehman S, Bari A, Maqsud A, Khan M . Juvenile rheumatoid arthritis. J Coll Physicians Surg Pak. 2013; 23(6):409-12. DOI: 06.2013/JCPSP.409412. View

Zuber Z, Turowska-Heydel D, Sobczyk M, Chudek J . Prevalence of HLA-B27 antigen in patients with juvenile idiopathic arthritis. Reumatologia. 2016; 53(3):125-30. PMC: 4847302. DOI: 10.5114/reum.2015.53133. View

de Jager W, Hoppenreijs E, Wulffraat N, Wedderburn L, Kuis W, Prakken B . Blood and synovial fluid cytokine signatures in patients with juvenile idiopathic arthritis: a cross-sectional study. Ann Rheum Dis. 2006; 66(5):589-98. PMC: 1954617. DOI: 10.1136/ard.2006.061853. View

10.

Oberle E, Harris J, Verbsky J . Polyarticular juvenile idiopathic arthritis - epidemiology and management approaches. Clin Epidemiol. 2014; 6:379-93. PMC: 4216020. DOI: 10.2147/CLEP.S53168. View

11.

Repo H, Jaattela M, Leirisalo-Repo M, Hurme M . Production of tumour necrosis factor and interleukin 1 by monocytes of patients with previous Yersinia arthritis. Clin Exp Immunol. 1988; 72(3):410-4. PMC: 1541576. View

12.

Lodhi N, Bashir M, Tipu H, Hussain M . Distribution of HLA-B*27 Subtypes in Patients with Ankylosing Spondylitis in Local Population. J Coll Physicians Surg Pak. 2019; 29(5):418-421. DOI: 10.29271/jcpsp.2019.05.418. View

13.

Wilbrink R, Spoorenberg A, Verstappen G, Kroese F . B Cell Involvement in the Pathogenesis of Ankylosing Spondylitis. Int J Mol Sci. 2021; 22(24). PMC: 8703482. DOI: 10.3390/ijms222413325. View

14.

Zaripova L, Midgley A, Christmas S, Beresford M, Baildam E, Oldershaw R . Juvenile idiopathic arthritis: from aetiopathogenesis to therapeutic approaches. Pediatr Rheumatol Online J. 2021; 19(1):135. PMC: 8383464. DOI: 10.1186/s12969-021-00629-8. View

15.

Mahmud S, Binstadt B . Autoantibodies in the Pathogenesis, Diagnosis, and Prognosis of Juvenile Idiopathic Arthritis. Front Immunol. 2019; 9:3168. PMC: 6339949. DOI: 10.3389/fimmu.2018.03168. View

16.

Keller C, Webb A, Davis J . Cytokines in the seronegative spondyloarthropathies and their modification by TNF blockade: a brief report and literature review. Ann Rheum Dis. 2003; 62(12):1128-32. PMC: 1754419. DOI: 10.1136/ard.2003.011023. View

17.

Moatter T, Aban M, Tabassum S, Shaikh U, Pervez S . Molecular analysis of human leukocyte antigen class I and class II allele frequencies and haplotype distribution in Pakistani population. Indian J Hum Genet. 2011; 16(3):149-53. PMC: 3009426. DOI: 10.4103/0971-6866.73408. View

18.

Wiegering V, Girschick H, Morbach H . B-cell pathology in juvenile idiopathic arthritis. Arthritis. 2011; 2010:759868. PMC: 3199973. DOI: 10.1155/2010/759868. View

19.

Varnavidou-Nicolaidou A, Karpasitou K, Georgiou D, Stylianou G, Kokkofitou A, Michalis C . HLA-B27 in the Greek Cypriot population: distribution of subtypes in patients with ankylosing spondylitis and other HLA-B27-related diseases. The possible protective role of B*2707. Hum Immunol. 2004; 65(12):1451-4. DOI: 10.1016/j.humimm.2004.08.177. View

20.

Stanevicha V, Eglite J, Zavadska D, Sochnevs A, Lazareva A, Guseinova D . HLA B27 allele types in homogeneous groups of juvenile idiopathic arthritis patients in Latvia. Pediatr Rheumatol Online J. 2010; 8:26. PMC: 2964715. DOI: 10.1186/1546-0096-8-26. View