Isolation of Human Antibodies Against Influenza B Neuraminidase and Mechanisms of Protection at the Airway Interface

Overview

Journal Immunity

Publisher Cell Press

Specialty Allergy & Immunology

Date 2024 Jun 1

PMID 38823390

Authors

Rachael M Wolters

James A Ferguson

Ivette A Nunez

Elaine E Chen

Ty Sornberger

Luke Myers

Svearike Oeverdieck

Sai Sundar Rajan Raghavan

Chandrahaas Kona

Laura S Handal

Trevor E Esilu

Edgar Davidson

Benjamin J Doranz

Taylor B Engdahl

Nurgun Kose

Lauren E Williamson

C Buddy Creech

Katherine N Gibson-Corley

Andrew B Ward

James E Crowe Jr

Affiliations

Soon will be listed here.

Abstract

Influenza B viruses (IBVs) comprise a substantial portion of the circulating seasonal human influenza viruses. Here, we describe the isolation of human monoclonal antibodies (mAbs) that recognized the IBV neuraminidase (NA) glycoprotein from an individual following seasonal vaccination. Competition-binding experiments suggested the antibodies recognized two major antigenic sites. One group, which included mAb FluB-393, broadly inhibited IBV NA sialidase activity, protected prophylactically in vivo, and bound to the lateral corner of NA. The second group contained an active site mAb, FluB-400, that broadly inhibited IBV NA sialidase activity and virus replication in vitro in primary human respiratory epithelial cell cultures and protected against IBV in vivo when administered systemically or intranasally. Overall, the findings described here shape our mechanistic understanding of the human immune response to the IBV NA glycoprotein through the demonstration of two mAb delivery routes for protection against IBV and the identification of potential IBV therapeutic candidates.

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