IgE Fc Receptor Positive T and B Lymphocytes in Patients with the Hyper IgE Syndrome

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 1985 Jan 1

PMID 3882288

Citations 2

Authors

L F Thompson

H L Spiegelberg

R H Buckley

Affiliations

Soon will be listed here.

Abstract

The percentages of peripheral blood lymphocytes (PBL), bearing Fc receptors for IgE (Fc epsilon R) and IgG (Fc gamma R) were determined in four patients with the hyper IgE syndrome by a rosette assay employing IgE and IgG coated fixed ox erythrocytes. The patients had 8 +/- 3% Fc epsilon R+ and 13 +/- 8% Fc gamma R+ PBL, compared to 1.2 +/- 1% Fc epsilon R+ and 17 +/- 4% Fc gamma R+ PBL for control donors. T cells were isolated by rosetting with neuraminidase treated sheep erythrocytes (EN). Indirect immunofluorescence with Lyt 3 monoclonal antibody (MoAb) to the sheep erythrocyte receptor, followed by rosetting for Fc epsilon R and Fc gamma R showed that the patients' T cells contained less than 0.1% Fc epsilon R+ and 1.4 +/- 0.2% Fc gamma R+ cells; T cells from the control subjects contained less than 0.1% Fc epsilon R+ and 11 +/- 4% Fc gamma R+ cells. The non-T (EN rosette depleted) cells of the patients included 56 +/- 18% sIgM+/sIgD+, 45 +/- 9% Fc epsilon R+ and 35 +/- 27% Fc gamma R+ cells. Indirect immunofluorescence with MoAb to IgM, IgD, and NK cells (antibody B73.1) followed by rosetting for Fc epsilon R and Fc gamma R, indicated that 92 +/- 2% of the Fc epsilon R+ cells and 9 +/- 7% of the Fc gamma R+ cells were B cells (mu+/delta+), while 3 +/- 4% of the Fc epsilon R+ and 30 +/- 23% of the Fc gamma R+ cells were NK cells (B73.1+). Thus, most of the Fc epsilon R+ non-T cells were B cells, and only a small fraction appeared to be NK cells. On the other hand, Fc gamma R+ B cells were outnumbered by Fc gamma R+ NK cells (B73.1+) by three to one. The data indicate that patients with the hyper IgE syndrome have increased numbers of Fc gamma R+ PBL, most of them being B cells, whereas their T cells contain less than 0.1% Fc epsilon R+ cells.

Citing Articles

In vitro production of IgE-binding factors by human mononuclear cells.

Delespesse G, Sarfati M Immunology. 1987; 60(1):103-10.

PMID: 3493203 PMC: 1453363.

Subset of natural killer cells is induced by immune complexes to display Fc receptors for IgE and IgA and demonstrates isotype regulatory function.

Kimata H, Saxon A J Clin Invest. 1988; 82(1):160-7.

PMID: 2455732 PMC: 303490. DOI: 10.1172/JCI113565.

References

Leung D, Rhodes A, Geha R . Enumeration of T cell subsets in atopic dermatitis using monoclonal antibodies. J Allergy Clin Immunol. 1981; 67(6):450-5. DOI: 10.1016/0091-6749(81)90098-1. View

Lamers M, Heckford S, Dickler H . Monoclonal anti-Fc IgG receptor antibodies trigger B lymphocyte function. Nature. 1982; 298(5870):178-80. DOI: 10.1038/298178a0. View

Perussia B, STARR S, Abraham S, Fanning V, Trinchieri G . Human natural killer cells analyzed by B73.1, a monoclonal antibody blocking Fc receptor functions. I. Characterization of the lymphocyte subset reactive with B73.1. J Immunol. 1983; 130(5):2133-41. View

Perussia B, Acuto O, Terhorst C, Faust J, Lazarus R, Fanning V . Human natural killer cells analyzed by B73.1, a monoclonal antibody blocking Fc receptor functions. II. Studies of B73.1 antibody-antigen interaction on the lymphocyte membrane. J Immunol. 1983; 130(5):2142-8. View

Phillips J, Babcock G . NKP-15: a monoclonal antibody reactive against purified human natural killer cells and granulocytes. Immunol Lett. 1983; 6(3):143-9. DOI: 10.1016/0165-2478(83)90096-2. View

Thompson L, Mellon M, Zeiger R, Spiegelberg H . Characterization with monoclonal antibodies of T lymphocytes bearing Fc receptors for IgE (T epsilon cells) and IgG (T gamma cells) in atopic patients. J Immunol. 1983; 131(6):2772-6. View

Buckley R, Wray B, Belmaker E . Extreme hyperimmunoglobulinemia E and undue susceptibility to infection. Pediatrics. 1972; 49(1):59-70. View

Buckley R, Fiscus S . Serum IgD and IgE concentrations in immunodeficiency diseases. J Clin Invest. 1975; 55(1):157-65. PMC: 301727. DOI: 10.1172/JCI107906. View

Ryan J, Arbeit R, Dickler H, Henkart P . Inhibition of lymphocyte mitogenesis by immobilized antigen-antibody complexes. J Exp Med. 1975; 142(4):814-26. PMC: 2189949. DOI: 10.1084/jem.142.4.814. View

10.

Sidman C, UNANUE E . Control of B-lymphocyte function. I. Inactivation of mitogenesis by interactions with surface immunoglobulin and Fc-receptor molecules. J Exp Med. 1976; 144(4):882-96. PMC: 2190431. DOI: 10.1084/jem.144.4.882. View

11.

Gonzalez-Molina A, Spiegelberg H . A subpopulation of normal human peripheral B lymphcytes that bind IgE. J Clin Invest. 1977; 59(4):616-24. PMC: 372265. DOI: 10.1172/JCI108679. View

12.

Moretta L, Webb S, Grossi C, Lydyard P, Cooper M . Functional analysis of two human T-cell subpopulations: help and suppression of B-cell responses by T cells bearing receptors for IgM or IgG. J Exp Med. 1977; 146(1):184-200. PMC: 2180738. DOI: 10.1084/jem.146.1.184. View

13.

Spiegelberg H, Dainer P . Fc receptors for IgG, IgM and IgE on human leukaemic lymphocytes. Clin Exp Immunol. 1979; 35(2):286-95. PMC: 1537643. View

14.

Yodoi J, Ishizaka K . Lymphocytes bearing Fc receptors for IgE. I. Presence of human and rat T lymphocytes with Fc epsilon receptors. J Immunol. 1979; 122(6):2577-83. View

15.

Spiegelberg H, OConnor R, Simon R, Mathison D . Lymphocytes with immunoglobulin E Fc receptors in patients with atopic disorders. J Clin Invest. 1979; 64(3):714-20. PMC: 372172. DOI: 10.1172/JCI109514. View

16.

Thompson L, Boss G, Spiegelberg H, Jansen I, OConnor R, Waldmann T . Ecto-5'-nucleotidase activity in T and B lymphocytes from normal subjects and patients with congenital X-linked agammaglobulinemia. J Immunol. 1979; 123(6):2475-8. View

17.

Yodoi J, Ishizaka K . Lymphocytes bearing Fc receptors for IgE. IV. Formation of IgE-binding factor by rat T lymphocytes. J Immunol. 1980; 124(3):1322-9. View

18.

Breard J, Reinherz E, Kung P, Goldstein G, Schlossman S . A monoclonal antibody reactive with human peripheral blood monocytes. J Immunol. 1980; 124(4):1943-8. View

19.

Reinherz E, Schlossman S . The differentiation and function of human T lymphocytes. Cell. 1980; 19(4):821-7. DOI: 10.1016/0092-8674(80)90072-0. View

20.

Kamoun M, Martin P, Hansen J, Brown M, Siadak A, Nowinski R . Identification of a human T lymphocyte surface protein associated with the E-rosette receptor. J Exp Med. 1981; 153(1):207-12. PMC: 2186049. DOI: 10.1084/jem.153.1.207. View