Human Major Histocompatibility Complex Class I Antigens: Residues 61-83 of the HLA-B7 Heavy Chain Specify an Alloreactive Site

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1985 Jan 1

PMID 3881768

Citations 12

Authors

L E Walker

T A Ketler

R A Houghten

G Schulz

A Chersi

R A Reisfeld

Affiliations

Soon will be listed here.

Abstract

A chemically synthesized peptide (sequence in text) homologous to residues 61-83 of the HLA-B7 heavy chain, induced antibodies that specifically recognized the HLA heavy chain-beta 2-microglobulin complex and the free heavy chain of the HLA-B7 antigen. These antibodies specifically immunoprecipitated the HLA-B7 beta 2-microglobulin complex solubilized from human lymphoblastoid cells by nonionic detergents and reacted with free HLA-B7 heavy chains in blots on nitrocellulose. These observations suggest that the antigenic conformation of this region of the HLA-B7 molecule is independent of the presence of beta 2-microglobulin and that amino acid residues 61-83 mimic an alloreactive site expressed by the HLA-B7 antigen.

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