Immunohistochemical Localisation of Tissue Plasminogen Activator and Urokinase in the Vessel Wall

Overview

Journal J Clin Pathol

Specialty Pathology

Date 1985 Feb 1

PMID 3881480

Citations 4

Authors

A Larsson

B ASTEDT

Affiliations

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Abstract

Immunoreactive plasminogen activators were studied in tissue sections using a peroxidase method and monospecific antibodies to tissue plasminogen activator produced by a melanoma. Tissue plasminogen activator reactivity was found in skin melanomas and in endothelial and smooth muscle cells of arteries and veins. Vessels of the umbilical cord showed higher reactivity than peripheral vessels. Only faint antiurokinase reactivity was found. By means of the fibrin slide technique, fibrinolytic activity could be shown in peripheral vessel walls but not in the umbilical cord, which suggests that immunoreactivity of tissue plasminogen activator bound to an inhibitor can also be demonstrated. This method may be a useful tool in further studies of tissue plasminogen activator in physiological as well as pathological processes.

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References

Svanberg L, Linell F, Pandolfi M, ASTEDT B . Plasminogen activators in ovarian tumours. Acta Pathol Microbiol Scand A. 1975; 83(2):193-8. DOI: 10.1111/j.1699-0463.1975.tb01374.x. View

ASTEDT B, Pandolfi M . On release and synthesis of fibrinolytic activators in human organ culture. Rev Eur Etud Clin Biol. 1972; 17(3):261-7. View

Holmberg L, Bladh B, ASTEDT B . Purification of urokinase by affinity chromatography. Biochim Biophys Acta. 1976; 445(1):215-22. DOI: 10.1016/0005-2744(76)90174-1. View

Wu M, ARIMURA G, YUNIS A . Purification and characterization of a plasminogen activator secreted by cultured human pancreatic carcinoma cells. Biochemistry. 1977; 16(9):1908-13. DOI: 10.1021/bi00628a023. View

Loskutoff D, Edgington T . Synthesis of a fibrinolytic activator and inhibitor by endothelial cells. Proc Natl Acad Sci U S A. 1977; 74(9):3903-7. PMC: 431779. DOI: 10.1073/pnas.74.9.3903. View

ASTEDT B, Barlow G, Holmberg L . Time-related release of various molecular forms of urokinase in tissue culture. Thromb Res. 1977; 11(2):149-53. DOI: 10.1016/0049-3848(77)90033-0. View

Holmberg L, Lecander I, Persson B, ASTEDT B . An inhibitor from placenta specifically binds urokinase and inhibits plasminogen activator released from ovarian carcinoma in tissue culture. Biochim Biophys Acta. 1978; 544(1):128-37. DOI: 10.1016/0304-4165(78)90216-7. View

Wu M, YUNIS A . Comparative studies on urokinase and plasminogen activator from cultured pancreatic carcinoma. Int J Biochem. 1979; 10(12):1001-6. DOI: 10.1016/0020-711x(79)90080-6. View

Vetterlein D, Bell T, Young P, Roblin R . Immunological quantitation and immunoadsorption of urokinase-like plasminogen activators secreted by human cells. J Biol Chem. 1980; 255(8):3665-72. View

10.

Rijken D, Wijngaards G, Welbergen J . Relationship between tissue plasminogen activator and the activators in blood and vascular wall. Thromb Res. 1980; 18(6):815-30. DOI: 10.1016/0049-3848(80)90204-2. View

11.

Naito S, Sueishi K, Hattori F, Tanaka K . Immunological analysis of plasminogen activators from cultured human cancer cells. Virchows Arch A Pathol Anat Histol. 1980; 387(3):251-7. DOI: 10.1007/BF00454828. View

12.

Loskutoff D, Edgington T . An inhibitor of plasminogen activator in rabbit endothelial cells. J Biol Chem. 1981; 256(9):4142-5. View

13.

Tarin D, Hoyt B, Evans D . Correlation of collagenase secretion with metastatic-colonization potential in naturally occurring murine mammary tumours. Br J Cancer. 1982; 46(2):266-78. PMC: 2011086. DOI: 10.1038/bjc.1982.192. View

14.

Nielsen L, Hansen J, Skriver L, Wilson E, Kaltoft K, Zeuthen J . Purification of zymogen to plasminogen activator from human glioblastoma cells by affinity chromatography with monoclonal antibody. Biochemistry. 1982; 21(25):6410-5. DOI: 10.1021/bi00268a014. View

15.

Salo T, Liotta L, Keski-Oja J, Turpeenniemi-Hujanen T, Tryggvason K . Secretion of basement membrane collagen degrading enzyme and plasminogen activator by transformed cells--role in metastasis. Int J Cancer. 1982; 30(5):669-73. DOI: 10.1002/ijc.2910300520. View

16.

Ljungner H, Holmberg L, Kjeldgaard A, Nilsson I, ASTEDT B . Immunological characterisation of plasminogen activators in the human vessel wall. J Clin Pathol. 1983; 36(9):1046-9. PMC: 498466. DOI: 10.1136/jcp.36.9.1046. View

17.

Levin E . Latent tissue plasminogen activator produced by human endothelial cells in culture: evidence for an enzyme-inhibitor complex. Proc Natl Acad Sci U S A. 1983; 80(22):6804-8. PMC: 390074. DOI: 10.1073/pnas.80.22.6804. View

18.

ASTRUP T, STERNDORFF I . An activator of plasminogen in normal urine. Proc Soc Exp Biol Med. 1952; 81(3):675-8. DOI: 10.3181/00379727-81-19983. View

19.

ASTRUP T . Fibrinolysis in the organism. Blood. 1956; 11(9):781-806. View

20.

PLOUG J, KJELDGAARD N . Urokinase an activator of plasminogen from human urine. I. Isolation and properties. Biochim Biophys Acta. 1957; 24(2):278-82. DOI: 10.1016/0006-3002(57)90194-4. View