In Vitro Antibacterial Activity and Secondary Metabolite Profiling of Endolichenic Fungi Isolated from Genus Parmotrema

Overview

Journal Curr Microbiol

Specialty Microbiology

Date 2024 May 29

PMID 38809483

Authors

Jyotsna Chakarwarti

Vandana Anand

Sanjeeva Nayaka

Suchi Srivastava

Affiliations

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Abstract

The endolichenic fungi are an unexplored group of organisms for the production of bioactive secondary metabolites. The aim of the present study is to determine the antibacterial potential of endolichenic fungi isolated from genus Parmotrema. The study is continuation of our previous work, wherein a total of 73 endolichenic fungi were isolated from the lichenized fungi, which resulted in 47 species under 23 genera. All the isolated endolichenic fungi were screened for preliminary antibacterial activity. Five endolichenic fungi-Daldinia eschscholtzii, Nemania diffusa, Preussia sp., Trichoderma sp. and Xylaria feejeensis, were selected for further antibacterial activity by disc diffusion method. The zone of inhibition ranged from 14.3 ± 0.1 to 23.2 ± 0.1. The chemical composition of the selected endolichenic fungi was analysed through GC-MS, which yielded a total of 108 compounds from all the selected five endolichenic fungi. Diethyl phthalate, 1-hexadecanol, dibutyl phthalate, n-tetracosanol-1, 1-nonadecene, pyrrol[1,2-a] pyrazine-1,4-dione, hexahydro-3-(2-methyl) and tetratetracontane were found to be common compounds among one or the other endolichenic fungi, which possibly were responsible for antibacterial activity. GC-MS data were further analysed through Principal Component Analysis which showed D. eschscholtzii to be with unique pattern of expression of metabolites. Compound confirmation test revealed coumaric acid to be responsible for antibacterial activity in D. eschscholtzii. So, the study proves that endolichenic fungi that inhabit lichenized fungal thalli could be a source of potential antibacterial compounds.

References

Baker R, Mahmud A, Miller I, Rajeev M, Rasambainarivo F, Rice B . Infectious disease in an era of global change. Nat Rev Microbiol. 2021; 20(4):193-205. PMC: 8513385. DOI: 10.1038/s41579-021-00639-z. View

Atanasov A, Zotchev S, Dirsch V, Supuran C . Natural products in drug discovery: advances and opportunities. Nat Rev Drug Discov. 2021; 20(3):200-216. PMC: 7841765. DOI: 10.1038/s41573-020-00114-z. View

Pham J, Yilma M, Feliz A, Majid M, Maffetone N, Walker J . A Review of the Microbial Production of Bioactive Natural Products and Biologics. Front Microbiol. 2019; 10:1404. PMC: 6596283. DOI: 10.3389/fmicb.2019.01404. View

Biosca E, Flores R, Santander R, Diez-Gil J, Barreno E . Innovative Approaches Using Lichen Enriched Media to Improve Isolation and Culturability of Lichen Associated Bacteria. PLoS One. 2016; 11(8):e0160328. PMC: 4975499. DOI: 10.1371/journal.pone.0160328. View

Muggia L, Fleischhacker A, Kopun T, Grube M . Extremotolerant fungi from alpine rock lichens and their phylogenetic relationships. Fungal Divers. 2016; 76:119-142. PMC: 4739527. DOI: 10.1007/s13225-015-0343-8. View

Arnold A, Miadlikowska J, Higgins K, Sarvate S, Gugger P, Way A . A phylogenetic estimation of trophic transition networks for ascomycetous fungi: are lichens cradles of symbiotrophic fungal diversification?. Syst Biol. 2010; 58(3):283-97. DOI: 10.1093/sysbio/syp001. View

Paranagama P, Wijeratne E, Burns A, Marron M, Gunatilaka M, Arnold A . Heptaketides from Corynespora sp. inhabiting the cavern beard lichen, Usnea cavernosa: first report of metabolites of an endolichenic fungus. J Nat Prod. 2007; 70(11):1700-5. DOI: 10.1021/np070466w. View

He J, Chen G, Gao H, Yang F, Li X, Peng T . Heptaketides with antiviral activity from three endolichenic fungal strains Nigrospora sp., Alternaria sp. and Phialophora sp. Fitoterapia. 2012; 83(6):1087-91. DOI: 10.1016/j.fitote.2012.05.002. View

Yang F, Chen G, Gao H, Li X, Wu Y, Guo L . Two new naphthalene derivatives from an endolichenic fungal strain Scopulariopsis sp. J Asian Nat Prod Res. 2012; 14(11):1059-63. DOI: 10.1080/10286020.2012.705278. View

10.

Yang B, Chen G, Li Y, Hu D, Guo L, Xiong P . A New Xanthone Glycoside from the Endolichenic Fungus Sporormiella irregularis. Molecules. 2016; 21(6). PMC: 6273424. DOI: 10.3390/molecules21060764. View

11.

Li X, Zhou Y, Zhu R, Chang W, Yuan H, Gao W . Identification and biological evaluation of secondary metabolites from the endolichenic fungus Aspergillus versicolor. Chem Biodivers. 2015; 12(4):575-92. DOI: 10.1002/cbdv.201400146. View

12.

Jha B, Shrestha M, Pandey D, Bhattarai T, Bhattarai H, Paudel B . Investigation of antioxidant, antimicrobial and toxicity activities of lichens from high altitude regions of Nepal. BMC Complement Altern Med. 2017; 17(1):282. PMC: 5445338. DOI: 10.1186/s12906-017-1797-x. View

13.

Jayaprakasha G, Rao L . Phenolic constituents from the lichen Parmotrema stuppeum (Nyl.) Hale and their antioxidant activity. Z Naturforsch C J Biosci. 2001; 55(11-12):1018-22. DOI: 10.1515/znc-2000-11-1227. View

14.

Padhi S, Das D, Panja S, Tayung K . Molecular Characterization and Antimicrobial Activity of an Endolichenic Fungus, Aspergillus sp. Isolated from Parmelia caperata of Similipal Biosphere Reserve, India. Interdiscip Sci. 2016; 9(2):237-246. DOI: 10.1007/s12539-016-0146-y. View

15.

Sahin F, Karaman I, Gulluce M, Ogutcu H, Sengul M, Adiguzel A . Evaluation of antimicrobial activities of Satureja hortensis L. J Ethnopharmacol. 2003; 87(1):61-5. DOI: 10.1016/s0378-8741(03)00110-7. View

16.

Bist V, Niranjan A, Ranjan M, Lehri A, Seem K, Srivastava S . Silicon-Solubilizing Media and Its Implication for Characterization of Bacteria to Mitigate Biotic Stress. Front Plant Sci. 2020; 11:28. PMC: 7061934. DOI: 10.3389/fpls.2020.00028. View

17.

Dubey D, Patnaik R, Ghosh G, Padhy R . In Vitro Antibacterial Activity, Gas Chromatography-Mass Spectrometry Analysis of Woodfordia fruticosa Kurz. Leaf Extract and Host Toxicity Testing With In Vitro Cultured Lymphocytes From Human Umbilical Cord Blood. Osong Public Health Res Perspect. 2014; 5(5):298-312. PMC: 4225590. DOI: 10.1016/j.phrp.2014.08.001. View

18.

Zhao J, Jiang L, Tang X, Peng L, Li X, Zhao G . Chemical Composition, Antimicrobial and Antioxidant Activities of the Flower Volatile Oils of Fagopyrum esculentum, Fagopyrum tataricum and Fagopyrum Cymosum. Molecules. 2018; 23(1). PMC: 6017370. DOI: 10.3390/molecules23010182. View

19.

Togashi N, Shiraishi A, Nishizaka M, Matsuoka K, Endo K, Hamashima H . Antibacterial activity of long-chain fatty alcohols against Staphylococcus aureus. Molecules. 2007; 12(2):139-48. PMC: 6149398. DOI: 10.3390/12020139. View

20.

Vambe M, Aremu A, Chukwujekwu J, Gruz J, Luterova A, Finnie J . Antibacterial, Mutagenic Properties and Chemical Characterisation of Sugar Bush ( Meisn.): A South African Native Shrub Species. Plants (Basel). 2020; 9(10). PMC: 7600625. DOI: 10.3390/plants9101331. View