Low-level LASER Therapy Accelerates Fungal Lesions Cicatrization by Increasing the Production of Th1 and Th2 Cytokines

Overview

Journal Photochem Photobiol Sci

Publisher Springer

Specialties Biology
Chemistry

Date 2024 May 28

PMID 38806861

Authors

Julianne Caravita Grisolia

Lauana Aparecida Santos

Nayara Andrade Dias

Luiz Cosme Cotta Malaquias

Eva Burger

Affiliations

Soon will be listed here.

Abstract

Objectives: Our study focused on the resolution of lesions caused by fungal infection using a subcutaneous air pouch model of infection.

Methods: We evaluated cell profile and cytokines, fungi viability, and the presence of fibroblasts and fibrocytes at the site of infection. Inoculation of P. brasiliensis (Pb) was performed using a subcutaneous air pouch model and the LLLT irradiation was performed on alternate days on the rear paws of mice for 10 days, after which the cells from the air pouch were collected and analyzed.

Results: In animals irradiated with LLLT, the influx of cells to the air pouch was reduced, but they were more activated and produced pro-inflammatory (IL-12, IL-17 and TNF-α) and neutrophil (PMN) activating cytokines (IL-8, GM-CSF and γ-IFN). A better resolution of the infection, evidenced by the reduction in the number of viable fungi with preserved morphology in the air pouch, and an increase in the number of fibrocytes, indicating a healing profile were also observed.

Conclusion: LLLT decreased the influx of PMN, but those presents were highly activated, with increased fungicidal activity. LLLT irradiation also resulted in earlier cicatrization at the site of infection, leading to a better outcome of the infection. These data are favorable to the use of LLLT as a complementary therapy in PCM.

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