Effect of Elevated Temperature and Hydrocortisone Addition on the Proliferation of Fibroblasts

Overview

Journal Histochem Cell Biol

Publisher Springer

Specialties Biochemistry
Cell Biology

Date 2024 May 27

PMID 38801536

Authors

Zuzana Pavlikova

Oldrich Zahradnicek

Anna Jelinek Michaelidesova

Jaromir Sramek

Marie Davidkova

Maria Hovorakova

Affiliations

Soon will be listed here.

Abstract

Hyperthermia along with hydrocortisone (HC) are proven teratogens that can negatively influence embryo development during early pregnancy. Proliferation of cells is one of the main developmental processes during the early embryogenesis. This study was focused on testing the effect of elevated temperature and HC addition on proliferation of cells in in vitro cultures. The V79-4 cell line was treated with HC and cultured in vitro at 37 °C or 39 °C, respectively. To reveal the effect of both factors, the proliferation of cells cultured under different conditions was evaluated using various approaches (colony formation assay, generation of growth curves, computation of doubling times, and mitotic index estimation). Our results indicate that a short-term exposure to elevated temperature slightly stimulates and a long-term exposure suppresses cell proliferation. However, HC (0.1 mg/ml) acts as a stimulator of cell proliferation. Interestingly, the interaction of HC and long-term elevated temperature (39 °C) exposure results in at least partial compensation of the negative impact of elevated temperature by HC addition and in higher proliferation if compared with cells cultured at 39 °C without addition of HC.

References

Grossfeld H, RAGAN C . Action of hydrocortisone on cells in tissue culture. Proc Soc Exp Biol Med. 1954; 86(1):63-8. DOI: 10.3181/00379727-86-21011. View

Macieira-Coelho A . Action of cortisone on human fibroblasts in vitro. Experientia. 1966; 22(6):390-1. DOI: 10.1007/BF01901156. View

Shah R, CHAUDHRY A . Hydrocortisone-induced cleft palate in hamsters. Teratology. 1973; 7(2):191-4. DOI: 10.1002/tera.1420070211. View

Fraser F, FAINSTAT T . Production of congenital defects in the off-spring of pregnant mice treated with cortisone; progress report. Pediatrics. 1951; 8(4):527-33. View

GEIGER R, DINGWALL J, ANDRUS W . The effect of cortisone on the growth of adult and embryonic tissues in vitro. Am J Med Sci. 1956; 231(4):427-36; passim. DOI: 10.1097/00000441-195604000-00007. View

Wan J, Wu W . Hyperthermia induced HIF-1a expression of lung cancer through AKT and ERK signaling pathways. J Exp Clin Cancer Res. 2016; 35(1):119. PMC: 4960890. DOI: 10.1186/s13046-016-0399-7. View

Hossain M, Matsuzaki K, Katakura M, Sugimoto N, Al Mamun A, Islam R . Direct exposure to mild heat promotes proliferation and neuronal differentiation of neural stem/progenitor cells in vitro. PLoS One. 2017; 12(12):e0190356. PMC: 5747471. DOI: 10.1371/journal.pone.0190356. View

RUNNER M . Comparative pharmacology in relation to teratogenesis. Fed Proc. 1967; 26(4):1131-6. View

Graham Jr J . Update on the gestational effects of maternal hyperthermia. Birth Defects Res. 2020; 112(12):943-952. DOI: 10.1002/bdr2.1696. View

10.

Suarez L, Felkner M, Hendricks K . The effect of fever, febrile illnesses, and heat exposures on the risk of neural tube defects in a Texas-Mexico border population. Birth Defects Res A Clin Mol Teratol. 2004; 70(10):815-9. DOI: 10.1002/bdra.20077. View

11.

Edwards M, Mulley R, Ring S, Wanner R . Mitotic cell death and delay of mitotic activity in guinea-pig embryos following brief maternal hyperthermia. J Embryol Exp Morphol. 1974; 32(3):593-602. View

12.

PINCUS G, Fischer A . THE GROWTH AND DEATH OF TISSUE CULTURES EXPOSED TO SUPRANORMAL TEMPERATURES. J Exp Med. 2009; 54(3):323-32. PMC: 2132010. DOI: 10.1084/jem.54.3.323. View

13.

Wust P, Hildebrandt B, Sreenivasa G, Rau B, Gellermann J, Riess H . Hyperthermia in combined treatment of cancer. Lancet Oncol. 2002; 3(8):487-97. DOI: 10.1016/s1470-2045(02)00818-5. View

14.

Hammer S, Sauer B, Spika I, Schraut C, Kleuser B, Schafer-Korting M . Glucocorticoids mediate differential anti-apoptotic effects in human fibroblasts and keratinocytes via sphingosine-1-phosphate formation. J Cell Biochem. 2004; 91(4):840-51. DOI: 10.1002/jcb.10766. View

15.

Yamaguchi S, Ohsaki Y, Nishigaki Y, Toyoshima E, Kikuchi K . Inhibition of human small cell lung cancer cell growth by methylprednisolone. Int J Oncol. 1999; 15(6):1185-90. DOI: 10.3892/ijo.15.6.1185. View

16.

Kline I, LEIGHTON J, Belkin M, ORR H . Some observations on the response of four established human cell strains to hydrocortisone in tissue culture. Cancer Res. 1957; 17(8):780-4. View

17.

Park H, Han S, Oh S, Kang H . Cellular responses to mild heat stress. Cell Mol Life Sci. 2004; 62(1):10-23. DOI: 10.1007/s00018-004-4208-7. View

18.

Oei A, van Leeuwen C, Ten Cate R, Rodermond H, Buist M, Stalpers L . Hyperthermia Selectively Targets Human Papillomavirus in Cervical Tumors via p53-Dependent Apoptosis. Cancer Res. 2015; 75(23):5120-9. DOI: 10.1158/0008-5472.CAN-15-0816. View

19.

Siddiqui S, Subramaniyan S, Kang D, Park J, Khan M, Choi H . Direct exposure to mild heat stress stimulates cell viability and heat shock protein expression in primary cultured broiler fibroblasts. Cell Stress Chaperones. 2020; 25(6):1033-1043. PMC: 7591668. DOI: 10.1007/s12192-020-01140-x. View

20.

BUXTON C, Atkinson W . Hormonal factors involved in the regulation of basal body temperature during the menstrual cycle and pregnancy. J Clin Endocrinol Metab. 1948; 8(7):544-9. DOI: 10.1210/jcem-8-7-544. View