The Impact of Altering the Concentration of Coffee Constituents on Their Anticancer Effect on Oral Squamous Cell Carcinoma Cell Line - Study

Overview

Journal Contemp Oncol (Pozn)

Publisher Termedia

Specialty Oncology

Date 2024 May 27

PMID 38800527

Authors

Asmaa Emad El-Din Mohammed Rashad

Mostafa Abdelwanis Mohamed Abdelaziz

Manar Abdulwaniss Mohammed Abdulaziz

Affiliations

Soon will be listed here.

Abstract

Introduction: Oral squamous cell carcinoma (OSCC) is one of the most common malignancies of the head and neck, which attracts much attention because of its increasing incidence and poor outcome. Coffee is one of the most popular beverages that are globally consumed. It consists of several phytochemical constituents, such as polyphenols, caffeine, and chlorogenic acid (CGA). Those constituents account for the potential effects on several diseases, including cancer. It has been reported that coffee exerts significant cytotoxicity against OSCC via inhibition of epidermal growth factor receptor tyrosine kinase (EGFR-TK) and up-regulation of apoptotic proteins, such as caspase-3 and caspase-9. The current study aims to measure the concentration of caffeine and CGA in 3 different types of coffee extracts, unroasted green coffee (GC), medium-roasted coffee (MRC), and decaffeinated coffee.

Material And Methods: The cytotoxic effect against OSCC-25 cell lines was evaluated and correlated with the concentration of constituents in each extract. The mechanisms of cytotoxicity were also studied by assessing the effect of each extract on caspase-3 and caspase-9 levels, in addition to the inhibitory effect on EGFR-TK.

Results: It was found that the caffeine concentration was higher in MRC than in GC because of the roasting process. However, the concentration of caspase-3 and -9 and the inhibitory effect on EGFR-TK were much higher in GC than MRC-treated cells because of the higher concentration of CGA.

Conclusions: Decaffeinated coffee exerts lower cytotoxic effects because it was totally deprived of caffeine and CGA during the decaffeination process.

References

Al Reef T, Ghanem E . Caffeine: Well-known as psychotropic substance, but little as immunomodulator. Immunobiology. 2018; 223(12):818-825. DOI: 10.1016/j.imbio.2018.08.011. View

Basar Gokcen B, Sanlier N . Coffee consumption and disease correlations. Crit Rev Food Sci Nutr. 2017; 59(2):336-348. DOI: 10.1080/10408398.2017.1369391. View

Salehi B, Lopez-Jornet P, Pons-Fuster Lopez E, Calina D, Sharifi-Rad M, Ramirez-Alarcon K . Plant-Derived Bioactives in Oral Mucosal Lesions: A Key Emphasis to Curcumin, Lycopene, Chamomile, , Green Tea and Coffee Properties. Biomolecules. 2019; 9(3). PMC: 6468600. DOI: 10.3390/biom9030106. View

Lu G, Huang S, Liu S, Liu P, Chou W, Lin W . Caffeine induces tumor cytotoxicity via the regulation of alternative splicing in subsets of cancer-associated genes. Int J Biochem Cell Biol. 2013; 47:83-92. DOI: 10.1016/j.biocel.2013.12.004. View

Kuntz S, Kunz C, Rudloff S . Inhibition of pancreatic cancer cell migration by plasma anthocyanins isolated from healthy volunteers receiving an anthocyanin-rich berry juice. Eur J Nutr. 2015; 56(1):203-214. DOI: 10.1007/s00394-015-1070-3. View

He B, Lin X, Tian F, Yu W, Qiao B . MiR-133a-3p Inhibits Oral Squamous Cell Carcinoma (OSCC) Proliferation and Invasion by Suppressing COL1A1. J Cell Biochem. 2017; 119(1):338-346. DOI: 10.1002/jcb.26182. View

He T, Guo X, Li X, Liao C, Yin W . Association between coffee intake and the risk of oral cavity cancer: a meta-analysis of observational studies. Eur J Cancer Prev. 2019; 29(1):80-88. DOI: 10.1097/CEJ.0000000000000515. View

Wang S, Yoon Y, Sung M, Hur H, Park J . Antiangiogenic properties of cafestol, a coffee diterpene, in human umbilical vein endothelial cells. Biochem Biophys Res Commun. 2012; 421(3):567-71. DOI: 10.1016/j.bbrc.2012.04.046. View

Zhang Y, Wang X, Cui D . Association between coffee consumption and the risk of oral cancer: a meta-analysis of observational studies. Int J Clin Exp Med. 2015; 8(7):11657-65. PMC: 4565385. View

10.

Ferlay J, Colombet M, Soerjomataram I, Mathers C, Parkin D, Pineros M . Estimating the global cancer incidence and mortality in 2018: GLOBOCAN sources and methods. Int J Cancer. 2018; 144(8):1941-1953. DOI: 10.1002/ijc.31937. View

11.

Liu H, Zhou Y, Tang L . Caffeine induces sustained apoptosis of human gastric cancer cells by activating the caspase‑9/caspase‑3 signalling pathway. Mol Med Rep. 2017; 16(3):2445-2454. PMC: 5547974. DOI: 10.3892/mmr.2017.6894. View

12.

Sharif K, Watad A, Bragazzi N, Adawi M, Amital H, Shoenfeld Y . Coffee and autoimmunity: More than a mere hot beverage!. Autoimmun Rev. 2017; 16(7):712-721. DOI: 10.1016/j.autrev.2017.05.007. View

13.

Boyanapalli S, Li W, Fuentes F, Guo Y, Ramirez C, Gonzalez X . Epigenetic reactivation of RASSF1A by phenethyl isothiocyanate (PEITC) and promotion of apoptosis in LNCaP cells. Pharmacol Res. 2016; 114:175-184. PMC: 5396455. DOI: 10.1016/j.phrs.2016.10.021. View

14.

Sharifi-Rad J, Ozleyen A, Tumer T, Oluwaseun Adetunji C, El Omari N, Balahbib A . Natural Products and Synthetic Analogs as a Source of Antitumor Drugs. Biomolecules. 2019; 9(11). PMC: 6920853. DOI: 10.3390/biom9110679. View

15.

Rivera C . Essentials of oral cancer. Int J Clin Exp Pathol. 2015; 8(9):11884-94. PMC: 4637760. View

16.

Galeone C, Tavani A, Pelucchi C, Turati F, Winn D, Levi F . Coffee and tea intake and risk of head and neck cancer: pooled analysis in the international head and neck cancer epidemiology consortium. Cancer Epidemiol Biomarkers Prev. 2010; 19(7):1723-36. PMC: 3047460. DOI: 10.1158/1055-9965.EPI-10-0191. View

17.

Tiwana M, Wu J, Hay J, Wong F, Cheung W, Olson R . 25 year survival outcomes for squamous cell carcinomas of the head and neck: population-based outcomes from a Canadian province. Oral Oncol. 2014; 50(7):651-6. DOI: 10.1016/j.oraloncology.2014.03.009. View

18.

Biazevic M, Toporcov T, Antunes J, Rotundo L, Brasileiro R, Brasilino de Carvalho M . Cumulative coffee consumption and reduced risk of oral and oropharyngeal cancer. Nutr Cancer. 2011; 63(3):350-6. DOI: 10.1080/01635581.2011.536065. View

19.

Goncalves D, de Carvalho N, Leite M, Courtes A, Hartmann D, Stefanello S . Caffeine and acetaminophen association: Effects on mitochondrial bioenergetics. Life Sci. 2017; 193:234-241. DOI: 10.1016/j.lfs.2017.10.039. View

20.

Liu W, Chang L . Caffeine induces matrix metalloproteinase-2 (MMP-2) and MMP-9 down-regulation in human leukemia U937 cells via Ca2+/ROS-mediated suppression of ERK/c-fos pathway and activation of p38 MAPK/c-jun pathway. J Cell Physiol. 2010; 224(3):775-85. DOI: 10.1002/jcp.22180. View