Anti-Melanogenic Effects of Extract Via P38 Signaling-Mediated Proteasomal Degradation of Tyrosinase

Overview

Journal Plants (Basel)

Date 2024 May 25

PMID 38794376

Authors

Soon Ho Choi

Hyunggun Kim

Jeon Hwang-Bo

Kyoung Mi Kim

Jeong Eun Kwon

Sung Ryul Lee

Sun Ha Hwang

Se Chan Kang

Yeong-Geun Lee

Affiliations

Soon will be listed here.

Abstract

fructus is widely used in traditional Oriental medicine for treating female genital disorders, male impotence, frigidity, and skin-related conditions in East Asia. However, the role of fructus extract (CMFE) in melanin synthesis is not well elucidated. This study aimed to investigate the anti-melanogenesis effect and mechanism of action of CMFE in α-MSH-stimulated B16F10 cells. Intracellular melanin content and tyrosinase activity were measured in α-MSH-stimulated B16F10 cells treated with various concentrations of CMFE (0.5-5 μg/mL). mRNA and protein levels of tyrosinase and MITF were evaluated using qRT-PCR and ting. CMFE's effect on the proteasomal degradation of tyrosinase was confirmed using a proteasomal degradation inhibitor, MG132. CMFE treatment activated p38, a protein associated with proteasomal degradation. Treatment with CMFE at up to 5 μg/mL showed no significant cytotoxicity. CMFE significantly reduced α-MSH-stimulated melanin production (43.29 ± 3.55% decrease, < 0.05) and cellular tyrosinase activity (31.14 ± 3.15% decrease, < 0.05). Although mRNA levels of MITF and tyrosinase increased, CMFE suppressed tyrosinase protein levels. The suppressive effect of CMFE on tyrosinase protein was blocked by MG132. CMFE inhibited melanogenesis by promoting the proteasome degradation of tyrosinase through p38 activation. These findings suggest that CMFE has the potential to be a natural whitening agent for inhibiting melanogenesis.

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