» Articles » PMID: 38792795

Metallo-Glycodendrimeric Materials Against Enterotoxigenic

Overview
Journal Microorganisms
Specialty Microbiology
Date 2024 May 25
PMID 38792795
Authors
Affiliations
Soon will be listed here.
Abstract

Conjugation of carbohydrates to nanomaterials has been extensively studied and recognized as an alternative in the biomedical field. Dendrimers synthesized with mannose at the end group and with entrapped zero-valent copper/silver could be a potential candidate against bacterial proliferation. This study is aimed at investigating the bactericidal activity of metal-glycodendrimers. The Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) reaction was used to synthesize a new mannosylated dendrimer containing 12 mannopyranoside residues in the periphery. The enterotoxigenic fimbriae 4 (ETEC:F4) viability, measured at 600 nm, showed the half-inhibitory concentration (IC) of metal-free glycodendrimers (D), copper-loaded glycodendrimers (D:Cu) and silver-loaded glycodendrimers (D:Ag) closed to 4.5 × 10, 3.5 × 10 and to 1.0 × 10 µg/mL, respectively, and minimum inhibitory concentration (MIC) of D, D:Cu and D:Ag of 2.0, 1.5 and 1.0 × 10 µg/mL, respectively. The release of bacteria contents onto broth and the inhibition of ETEC:F4 biofilm formation increased with the number of metallo-glycodendrimer materials, with a special interest in silver-containing nanomaterial, which had the highest activity, suggesting that glycodendrimer-based materials interfered with bacteria-bacteria or bacteria-polystyrene interactions, with bacteria metabolism and can disrupt bacteria cell walls. Our findings identify metal-mannose-dendrimers as potent bactericidal agents and emphasize the effect of entrapped zero-valent metal against ETEC:F4.

Citing Articles

Silver and Copper Nanoparticles Hosted by Carboxymethyl Cellulose Reduce the Infective Effects of Enterotoxigenic :F4 on Porcine Intestinal Enterocyte IPEC-J2.

Tchoumi Neree A, Noori F, Azzouz A, Costa M, Fairbrother J, Mateescu M Microorganisms. 2024; 12(10).

PMID: 39458335 PMC: 11509932. DOI: 10.3390/microorganisms12102026.

References
1.
de la Mata F, Gomez R, Cano J, Sanchez-Nieves J, Ortega P, Gallego S . Carbosilane dendritic nanostructures, highly versatile platforms for pharmaceutical applications. Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2022; 15(3):e1871. DOI: 10.1002/wnan.1871. View

2.
Matias J, Pastor Y, Irache J, Gamazo C . Protective Passive Immunity in ETEC-Challenged Neonatal Mice Conferred by Orally Immunized Dams with Nanoparticles Containing Homologous Outer Membrane Vesicles. Vaccines (Basel). 2020; 8(2). PMC: 7350024. DOI: 10.3390/vaccines8020286. View

3.
Zajac M, Kotynska J, Zambrowski G, Breczko J, Deptula P, Ciesluk M . Exposure to polystyrene nanoparticles leads to changes in the zeta potential of bacterial cells. Sci Rep. 2023; 13(1):9552. PMC: 10260929. DOI: 10.1038/s41598-023-36603-5. View

4.
Hrala M, Bosak J, Micenkova L, Krenova J, Lexa M, Pirkova V . Escherichia coli Strains Producing Selected Bacteriocins Inhibit Porcine Enterotoxigenic Escherichia coli (ETEC) under both and Conditions. Appl Environ Microbiol. 2021; 87(14):e0312120. PMC: 8231719. DOI: 10.1128/AEM.03121-20. View

5.
Sentamu D, Kungu J, Dione M, Thomas L . Prevention of human exposure to livestock faecal waste in the household: a scoping study of interventions conducted in sub-Saharan Africa. BMC Public Health. 2023; 23(1):1613. PMC: 10463677. DOI: 10.1186/s12889-023-16567-x. View