Functionalized Ionic Liquids-Modified Metal-Organic Framework Material Boosted the Enzymatic Performance of Lipase

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2024 May 25

PMID 38792242

Authors

Liran Ji

Wei Zhang

Yifei Zhang

Binbin Nian

Yi Hu

Affiliations

Soon will be listed here.

Abstract

The development of immobilized enzymes with high activity and stability is critical. Metal-organic frameworks (MOFs) have attracted much academic and industrial interest in the field of enzyme immobilization due to their unique properties. In this study, the amino-functionalized ionic liquid (NIL)-modified metal-organic framework (UiO-66-NH) was prepared to immobilize lipase (CRL), using dialdehyde starch (DAS) as the cross-linker. The results of the Fourier transform infrared (FT-IR) spectra, X-ray powder diffraction (XRD), and scanning electronic microscopy (SEM) confirmed that the NIL was successfully grafted to UiO-66-NH. The CRL immobilized on NIL-modified UiO-66-NH (UiO-66-NH-NIL-DAS@CRL) exhibited satisfactory activity recovery (79.33%), stability, reusability, and excellent organic solvent tolerance. The research results indicated that ionic liquid-modified UiO-66-NH had practical potential for application in enzyme immobilization.

References

Xing C, Mei P, Mu Z, Li B, Feng X, Zhang Y . Enhancing Enzyme Activity by the Modulation of Covalent Interactions in the Confined Channels of Covalent Organic Frameworks. Angew Chem Int Ed Engl. 2022; 61(21):e202201378. DOI: 10.1002/anie.202201378. View

Wang S, Li S, Liu R, Zhang W, Xu H, Hu Y . Immobilization of Interfacial Activated Lipase Onto Magnetic Chitosan Using Dialdehyde Cellulose as Cross-Linking Agent. Front Bioeng Biotechnol. 2022; 10:946117. PMC: 9340543. DOI: 10.3389/fbioe.2022.946117. View

Wan X, Tang S, Xiang X, Huang H, Hu Y . Immobilization of Candida antarctic Lipase B on Functionalized Ionic Liquid Modified MWNTs. Appl Biochem Biotechnol. 2017; 183(3):807-819. DOI: 10.1007/s12010-017-2465-9. View

Li M, Dai X, Li A, Qi Q, Wang W, Cao J . Preparation and Characterization of Magnetic Metal-Organic Frameworks Functionalized by Ionic Liquid as Supports for Immobilization of Pancreatic Lipase. Molecules. 2022; 27(20). PMC: 9609868. DOI: 10.3390/molecules27206800. View

Gao C, Hou J, Xu P, Guo L, Chen X, Hu G . Programmable biomolecular switches for rewiring flux in Escherichia coli. Nat Commun. 2019; 10(1):3751. PMC: 6704175. DOI: 10.1038/s41467-019-11793-7. View

Chen L, Yu F, Shen X, Duan C . N-CND modified NH-UiO-66 for photocatalytic CO conversion under visible light by a photo-induced electron transfer process. Chem Commun (Camb). 2019; 55(33):4845-4848. DOI: 10.1039/c9cc02193k. View

Chong S, Wang T, Cheng L, Lv H, Ji M . Metal-Organic Framework MIL-101-NH-Supported Acetate-Based Butylimidazolium Ionic Liquid as a Highly Efficient Heterogeneous Catalyst for the Synthesis of 3-Aryl-2-oxazolidinones. Langmuir. 2018; 35(2):495-503. DOI: 10.1021/acs.langmuir.8b03153. View

Liu J, Xie Y, Peng C, Yu G, Zhou J . Molecular Understanding of Laccase Adsorption on Charged Self-Assembled Monolayers. J Phys Chem B. 2017; 121(47):10610-10617. DOI: 10.1021/acs.jpcb.7b08738. View

Suo H, Geng X, Sun Y, Zhang L, Yang J, Yang F . Surface Modification of Magnetic ZIF-90 Nanoparticles Improves the Microenvironment of Immobilized Lipase and Its Application in Esterification. Langmuir. 2022; 38(49):15384-15393. DOI: 10.1021/acs.langmuir.2c02672. View

10.

Rodrigues R, Virgen-Ortiz J, S Dos Santos J, Berenguer-Murcia A, Alcantara A, Barbosa O . Immobilization of lipases on hydrophobic supports: immobilization mechanism, advantages, problems, and solutions. Biotechnol Adv. 2019; 37(5):746-770. DOI: 10.1016/j.biotechadv.2019.04.003. View

11.

Virgen-Ortiz J, S Dos Santos J, Berenguer-Murcia A, Barbosa O, Rodrigues R, Fernandez-Lafuente R . Polyethylenimine: a very useful ionic polymer in the design of immobilized enzyme biocatalysts. J Mater Chem B. 2020; 5(36):7461-7490. DOI: 10.1039/c7tb01639e. View

12.

Diao W, Guo L, Ding Q, Gao C, Hu G, Chen X . Reprogramming microbial populations using a programmed lysis system to improve chemical production. Nat Commun. 2021; 12(1):6886. PMC: 8617184. DOI: 10.1038/s41467-021-27226-3. View

13.

Liang W, Wied P, Carraro F, Sumby C, Nidetzky B, Tsung C . Metal-Organic Framework-Based Enzyme Biocomposites. Chem Rev. 2021; 121(3):1077-1129. DOI: 10.1021/acs.chemrev.0c01029. View

14.

Sojitra U, Nadar S, Rathod V . Immobilization of pectinase onto chitosan magnetic nanoparticles by macromolecular cross-linker. Carbohydr Polym. 2016; 157:677-685. DOI: 10.1016/j.carbpol.2016.10.018. View

15.

Pencreach G, Baratti J . Activity of Pseudomonas cepacia lipase in organic media is greatly enhanced after immobilization on a polypropylene support. Appl Microbiol Biotechnol. 1997; 47(6):630-5. DOI: 10.1007/s002530050986. View

16.

Bilal M, Adeel M, Rasheed T, Zhao Y, Iqbal H . Emerging contaminants of high concern and their enzyme-assisted biodegradation - A review. Environ Int. 2019; 124:336-353. DOI: 10.1016/j.envint.2019.01.011. View

17.

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

18.

Cea M, Gonzalez M, Abarzua M, Navia R . Enzymatic esterification of oleic acid by Candida rugosa lipase immobilized onto biochar. J Environ Manage. 2019; 242:171-177. DOI: 10.1016/j.jenvman.2019.04.013. View

19.

Jamshidifard S, Koushkbaghi S, Hosseini S, Rezaei S, Karamipour A, Jafari Rad A . Incorporation of UiO-66-NH2 MOF into the PAN/chitosan nanofibers for adsorption and membrane filtration of Pb(II), Cd(II) and Cr(VI) ions from aqueous solutions. J Hazard Mater. 2019; 368:10-20. DOI: 10.1016/j.jhazmat.2019.01.024. View

20.

Suo H, Gao Z, Xu L, Xu C, Yu D, Xiang X . Synthesis of functional ionic liquid modified magnetic chitosan nanoparticles for porcine pancreatic lipase immobilization. Mater Sci Eng C Mater Biol Appl. 2019; 96:356-364. DOI: 10.1016/j.msec.2018.11.041. View