Antimicrobial and Antibiofilm Potential of Green-Synthesized Graphene-Silver Nanocomposite Against Multidrug-Resistant Nosocomial Pathogens

Overview

Journal Biomedicines

Specialty Biomedical Engineering

Date 2024 May 25

PMID 38791065

Authors

Preeti Negi

Jatin Chadha

Kusum Harjai

Vijay Singh Gondil

Seema Kumari

Khem Raj

Affiliations

Soon will be listed here.

Abstract

Hospital-acquired infections (HAIs) pose a significant risk to global health, impacting millions of individuals globally. These infections have increased rates of morbidity and mortality due to the prevalence of widespread antimicrobial resistance (AMR). Graphene-based nanoparticles (GBNs) are known to possess extensive antimicrobial properties by inflicting damage to the cell membrane, suppressing virulence, and inhibiting microbial biofilms. Developing alternative therapies for HAIs and addressing AMR can be made easier and more affordable by combining nanoparticles with medicinal plants harboring antimicrobial properties. Hence, this study was undertaken to develop a novel graphene-silver nanocomposite via green synthesis using plant extract as a reducing agent. The resulting nanocomposite comprised silver nanoparticles embedded in graphene sheets. The antibacterial and antifungal properties of graphene-silver nanocomposites were investigated against several nosocomial pathogens, namely, , , , , , and . The nanocomposite displayed broad-range antimicrobial potential against the test pathogens, with minimum inhibitory concentrations (MICs) ranging between 31.25 and 125.0 µg/mL, and biofilm inhibition up to 80-96%. Moreover, nanocomposite-functionalized urinary catheters demonstrated hemocompatibility towards sheep erythrocytes and imparted anti-fouling activity to the biomaterial, while also displaying biocompatibility towards HEK 293 cells. Collectively, this investigation highlights the possible application of green-synthesized GBNs as an effective alternative to conventional antibiotics for combating multidrug-resistant pathogens.

Citing Articles

Efficient Defect-Driven Cation Exchange beyond the Nanoscale Semiconductors toward Antibacterial Functionalization.

Polivtseva S, Volobujeva O, Kuznietsov I, Kaupmees R, Danilson M, Krustok J ACS Appl Mater Interfaces. 2024; 16(45):62871-62882.

PMID: 39475460 PMC: 11565473. DOI: 10.1021/acsami.4c11425.

Revitalizing common drugs for antibacterial, quorum quenching, and antivirulence potential against : in vitro and in silico insights.

Chadha J, Mudgil U, Khullar L, Ahuja P, Harjai K 3 Biotech. 2024; 14(10):219.

PMID: 39239248 PMC: 11371971. DOI: 10.1007/s13205-024-04070-y.

References

Lara H, Ixtepan-Turrent L, Yacaman M, Lopez-Ribot J . Inhibition of Biofilm Formation on Medical and Environmental Surfaces by Silver Nanoparticles. ACS Appl Mater Interfaces. 2020; 12(19):21183-21191. PMC: 8243355. DOI: 10.1021/acsami.9b20708. View

Li C, Wang X, Chen F, Zhang C, Zhi X, Wang K . The antifungal activity of graphene oxide-silver nanocomposites. Biomaterials. 2013; 34(15):3882-90. DOI: 10.1016/j.biomaterials.2013.02.001. View

Pulingam T, Thong K, Appaturi J, Lai C, Leo B . Mechanistic actions and contributing factors affecting the antibacterial property and cytotoxicity of graphene oxide. Chemosphere. 2021; 281:130739. DOI: 10.1016/j.chemosphere.2021.130739. View

Chadha J, Ravi , Singh J, Chhibber S, Harjai K . Gentamicin Augments the Quorum Quenching Potential of Cinnamaldehyde and Protects From Infection. Front Cell Infect Microbiol. 2022; 12:899566. PMC: 9240785. DOI: 10.3389/fcimb.2022.899566. View

Anuta V, Talianu M, Dinu-Pirvu C, Ghica M, Prisada R, Albu Kaya M . Molecular Mapping of Antifungal Mechanisms Accessing Biomaterials and New Agents to Target Oral Candidiasis. Int J Mol Sci. 2022; 23(14). PMC: 9320712. DOI: 10.3390/ijms23147520. View

Burdusel A, Gherasim O, Grumezescu A, Mogoanta L, Ficai A, Andronescu E . Biomedical Applications of Silver Nanoparticles: An Up-to-Date Overview. Nanomaterials (Basel). 2018; 8(9). PMC: 6163202. DOI: 10.3390/nano8090681. View

Silva-Dias A, Miranda I, Branco J, Monteiro-Soares M, Pina-Vaz C, Rodrigues A . Adhesion, biofilm formation, cell surface hydrophobicity, and antifungal planktonic susceptibility: relationship among Candida spp. Front Microbiol. 2015; 6:205. PMC: 4357307. DOI: 10.3389/fmicb.2015.00205. View

Fisher L, Hook A, Ashraf W, Yousef A, Barrett D, Scurr D . Biomaterial modification of urinary catheters with antimicrobials to give long-term broadspectrum antibiofilm activity. J Control Release. 2015; 202:57-64. DOI: 10.1016/j.jconrel.2015.01.037. View

Chadha J, Khullar L, Gulati P, Chhibber S, Harjai K . Repurposing albendazole as a potent inhibitor of quorum sensing-regulated virulence factors in Pseudomonas aeruginosa: Novel prospects of a classical drug. Microb Pathog. 2023; 186:106468. DOI: 10.1016/j.micpath.2023.106468. View

10.

Nori P, Cowman K, Chen V, Bartash R, Szymczak W, Madaline T . Bacterial and fungal coinfections in COVID-19 patients hospitalized during the New York City pandemic surge. Infect Control Hosp Epidemiol. 2020; 42(1):84-88. PMC: 7417979. DOI: 10.1017/ice.2020.368. View

11.

Vazquez-Munoz R, Borrego B, Juarez-Moreno K, Garcia-Garcia M, Mota Morales J, Bogdanchikova N . Toxicity of silver nanoparticles in biological systems: Does the complexity of biological systems matter?. Toxicol Lett. 2017; 276:11-20. DOI: 10.1016/j.toxlet.2017.05.007. View

12.

Chadha J, Ravi , Singh J, Harjai K . α-Terpineol synergizes with gentamicin to rescue Caenorhabditis elegans from Pseudomonas aeruginosa infection by attenuating quorum sensing-regulated virulence. Life Sci. 2022; 313:121267. DOI: 10.1016/j.lfs.2022.121267. View

13.

Wierzbicki M, Jaworski S, Sawosz E, Jung A, Gielerak G, Jaremek H . Graphene Oxide in a Composite with Silver Nanoparticles Reduces the Fibroblast and Endothelial Cell Cytotoxicity of an Antibacterial Nanoplatform. Nanoscale Res Lett. 2019; 14(1):320. PMC: 6787127. DOI: 10.1186/s11671-019-3166-9. View

14.

Mohammadi M, Shahisaraee S, Tavajjohi A, Pournoori N, Muhammadnejad S, Mohammadi S . Green synthesis of silver nanoparticles using and extracts: characterisation, cell cytotoxicity, and its antifungal activity against in comparison to fluconazole. IET Nanobiotechnol. 2019; 13(2):114-119. PMC: 8676021. DOI: 10.1049/iet-nbt.2018.5146. View

15.

Rokaya D, Srimaneepong V, Qin J, Siraleartmukul K, Siriwongrungson V . Graphene Oxide/Silver Nanoparticle Coating Produced by Electrophoretic Deposition Improved the Mechanical and Tribological Properties of NiTi Alloy for Biomedical Applications. J Nanosci Nanotechnol. 2019; 19(7):3804-3810. DOI: 10.1166/jnn.2019.16327. View

16.

Tene T, Guevara M, Benalcazar Palacios F, Morocho Barrionuevo T, Vacacela Gomez C, Bellucci S . Optical properties of graphene oxide. Front Chem. 2023; 11:1214072. PMC: 10397392. DOI: 10.3389/fchem.2023.1214072. View

17.

Srisang S, Boongird A, Ungsurungsie M, Wanasawas P, Nasongkla N . Biocompatibility and stability during storage of Foley urinary catheters coated chlorhexidine loaded nanoparticles by nanocoating: in vitro and in vivo evaluation. J Biomed Mater Res B Appl Biomater. 2020; 109(4):496-504. DOI: 10.1002/jbm.b.34718. View

18.

. Global burden of bacterial antimicrobial resistance in 2019: a systematic analysis. Lancet. 2022; 399(10325):629-655. PMC: 8841637. DOI: 10.1016/S0140-6736(21)02724-0. View

19.

Tang K, Millar B, Moore J . Antimicrobial Resistance (AMR). Br J Biomed Sci. 2023; 80:11387. PMC: 10336207. DOI: 10.3389/bjbs.2023.11387. View

20.

Zhang P, Wang H, Zhang X, Xu W, Li Y, Li Q . Graphene film doped with silver nanoparticles: self-assembly formation, structural characterizations, antibacterial ability, and biocompatibility. Biomater Sci. 2015; 3(6):852-60. DOI: 10.1039/c5bm00058k. View