Insights into the Antioxidant, Anti-inflammatory and Anti-microbial Potential of Nigella Sativa Essential Oil Against Oral Pathogens

Overview

Journal Sci Rep

Specialty Science

Date 2024 May 24

PMID 38789533

Authors

Shaeesta Khaleelahmed Bhavikatti

Siti Lailatul Akmar Zainuddin

Rosmaliza Binti Ramli

Sameer J Nadaf

Padma B Dandge

Masidd Khalate

Mohmed Isaqali Karobari

Affiliations

Soon will be listed here.

Abstract

Oral disorders can exert systemic ramifications beyond their localized effects on dental tissues, implicating a wide array of physiological conditions. The utilization of essential oils (EOs) for protection of oral health represents a longstanding practice. Consequently, in this investigation, essential oil derived from Nigella sativa seeds (NSEO) underwent isolation via the hydro-distillation process, followed by a comprehensive evaluation of its antioxidant, anti-inflammatory, anti-fungal, antibacterial activities, and cytocompatibility. The isolated NSEO manifested as a pale-yellow substance and was found to harbor a diverse spectrum of bioactive constituents, including steroids, triterpenoids, flavonoids, phenols, proteins, alkaloids, tannin, sesquiterpenoid hydrocarbons, monoterpenoid alcohol, and monoterpenoid ketone (thymoquinone). Notably, the total phenolic content (TPC) and total flavonoid content (TFC) of NSEO were quantified at 641.23 μg GAE/gm and 442.25 μg QE/g, respectively. Furthermore, NSEO exhibited concentration-dependent inhibition of protein denaturation, HRBC membrane stabilization, and hemolysis inhibition. Comparative analysis revealed that NSEO and chlorhexidine (CHX) 0.2% displayed substantial inhibition of hemolysis compared to aspirin. While NSEO and CHX 0.2% demonstrated analogous antibacterial activity against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa, NSEO showcased heightened efficacy against Lactobacillus acidophilus and Candida albicans. Additionally, NSEO exhibited pronounced effects against periodontal pathogens such as Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, and Prevotella intermedia. Importantly, no cytotoxicity was observed on human gingival fibroblast cell lines. These findings underscore the potential of NSEO as a potent antibacterial and antifungal agent in the management of oral microbial pathogens, thereby offering avenues for the development of innovative therapies targeting diverse oral inflammatory conditions. Nevertheless, further investigations are imperative to unlock its full therapeutic repertoire.

References

Hannan M, Rahman M, Sohag A, Uddin M, Dash R, Sikder M . Black Cumin ( L.): A Comprehensive Review on Phytochemistry, Health Benefits, Molecular Pharmacology, and Safety. Nutrients. 2021; 13(6). PMC: 8225153. DOI: 10.3390/nu13061784. View

Deepa A, Nair B, Sivakumar T, Joseph A . Uncommon opportunistic fungal infections of oral cavity: A review. J Oral Maxillofac Pathol. 2014; 18(2):235-43. PMC: 4196293. DOI: 10.4103/0973-029X.140765. View

Ardiana M, Pikir B, Santoso A, Hermawan H, Al-Farabi M . Effect of Supplementation on Oxidative Stress and Antioxidant Parameters: A Meta-Analysis of Randomized Controlled Trials. ScientificWorldJournal. 2020; 2020:2390706. PMC: 7225850. DOI: 10.1155/2020/2390706. View

Tantivitayakul P, Kaypetch R, Muadchiengka T . Thymoquinone inhibits biofilm formation and virulence properties of periodontal bacteria. Arch Oral Biol. 2020; 115:104744. DOI: 10.1016/j.archoralbio.2020.104744. View

Nazzaro F, Fratianni F, Coppola R, De Feo V . Essential Oils and Antifungal Activity. Pharmaceuticals (Basel). 2017; 10(4). PMC: 5748643. DOI: 10.3390/ph10040086. View

Prabuseenivasan S, Jayakumar M, Ignacimuthu S . In vitro antibacterial activity of some plant essential oils. BMC Complement Altern Med. 2006; 6:39. PMC: 1693916. DOI: 10.1186/1472-6882-6-39. View

Kaur G, Kathariya R, Bansal S, Singh A, Shahakar D . Dietary antioxidants and their indispensable role in periodontal health. J Food Drug Anal. 2017; 24(2):239-246. PMC: 9339570. DOI: 10.1016/j.jfda.2015.11.003. View

Jesus F, Ferreiro L, Bizzi K, Loreto E, Pilotto M, Ludwig A . In vitro activity of carvacrol and thymol combined with antifungals or antibacterials against Pythium insidiosum. J Mycol Med. 2015; 25(2):e89-93. DOI: 10.1016/j.mycmed.2014.10.023. View

Kabir Y, Akasaka-Hashimoto Y, Kubota K, Komai M . Volatile compounds of black cumin ( L.) seeds cultivated in Bangladesh and India. Heliyon. 2020; 6(10):e05343. PMC: 7610257. DOI: 10.1016/j.heliyon.2020.e05343. View

10.

Tawfig A . The effect of Nigella sativa extracts against Porphyromonas gingivalis isolated from periodontitis patients. Eur Rev Med Pharmacol Sci. 2023; 27(21):10642-10650. DOI: 10.26355/eurrev_202311_34344. View

11.

Ghahramanloo K, Kamalidehghan B, Javar H, Widodo R, Majidzadeh K, Noordin M . Comparative analysis of essential oil composition of Iranian and Indian L. extracted using supercritical fluid extraction and solvent extraction. Drug Des Devel Ther. 2017; 11:2221-2226. PMC: 5546729. DOI: 10.2147/DDDT.S87251. View

12.

Bastug A, Tomruk C, Guzel E, Ozdemir I, Duygu G, Kutan E . The effect of local application of thymoquinone, 's bioactive component, on bone healing in experimental bone defects infected with . J Periodontal Implant Sci. 2022; 52(3):206-219. PMC: 9253281. DOI: 10.5051/jpis.2101360068. View

13.

Aryal S, Baniya M, Danekhu K, Kunwar P, Gurung R, Koirala N . Total Phenolic Content, Flavonoid Content and Antioxidant Potential of Wild Vegetables from Western Nepal. Plants (Basel). 2019; 8(4). PMC: 6524357. DOI: 10.3390/plants8040096. View

14.

Kapil H, Suresh D, Bathla S, Arora K . Assessment of clinical efficacy of locally delivered 0.2% Thymoquinone gel in the treatment of periodontitis. Saudi Dent J. 2018; 30(4):348-354. PMC: 6128318. DOI: 10.1016/j.sdentj.2018.06.001. View

15.

Castro M, Duarte N, Nascimento P, Magno M, Fagundes N, Flores-Mir C . Antioxidants as Adjuvants in Periodontitis Treatment: A Systematic Review and Meta-Analysis. Oxid Med Cell Longev. 2019; 2019:9187978. PMC: 6679881. DOI: 10.1155/2019/9187978. View

16.

Soobrattee M, Neergheen V, Luximon-Ramma A, Aruoma O, Bahorun T . Phenolics as potential antioxidant therapeutic agents: mechanism and actions. Mutat Res. 2005; 579(1-2):200-13. DOI: 10.1016/j.mrfmmm.2005.03.023. View

17.

Mohammed S, Amin H, Aziz S, Sha A, Hassan S, Abdul Aziz J . Structural Characterization, Antimicrobial Activity, and Cytotoxicity Effect of Black Seed Oil. Evid Based Complement Alternat Med. 2019; 2019:6515671. PMC: 6721493. DOI: 10.1155/2019/6515671. View

18.

Bordoni L, Fedeli D, Nasuti C, Maggi F, Papa F, Wabitsch M . Antioxidant and Anti-Inflammatory Properties of Oil in Human Pre-Adipocytes. Antioxidants (Basel). 2019; 8(2). PMC: 6406245. DOI: 10.3390/antiox8020051. View

19.

Heredia D, Green I, Klaasen J, Rahiman F . Importance and Relevance of Phytochemicals Present in . Scientifica (Cairo). 2022; 2022:5793436. PMC: 8850083. DOI: 10.1155/2022/5793436. View

20.

Lodhia M, Bhatt K, Thaker V . Antibacterial activity of essential oils from palmarosa, evening primrose, lavender and tuberose. Indian J Pharm Sci. 2010; 71(2):134-6. PMC: 2839398. DOI: 10.4103/0250-474X.54278. View