Alternariol Monomethyl-Ether Induces Toxicity Via Cell Death and Oxidative Stress in Swine Intestinal Epithelial Cells

Overview

Journal Toxins (Basel)

Publisher MDPI

Specialty Toxicology

Date 2024 May 24

PMID 38787075

Authors

Daniela Eliza Marin

Valeria Cristina Bulgaru

AnaMaria Pertea

Iulian Alexandru Grosu

Gina Cecilia Pistol

Ionelia Taranu

Affiliations

Soon will be listed here.

Abstract

Alternariol monomethyl-ether (AME), together with altenuene and alternariol, belongs to the mycotoxins group, which can contaminate different substrates, including cereals. The aim of the present study was to obtain a deeper understanding concerning the effects of AME on pig intestinal health using epithelial intestinal cell lines as the data concerning the possible effects of toxins on swine are scarce and insufficient for assessing the risk represented by toxins for animal health. Our results have shown a dose-related effect on IPEC-1 cell viability, with an IC50 value of 10.5 μM. Exposure to the toxin induced an increase in total apoptotic cells, suggesting that AME induces programmed cell death through apoptosis based on caspase-3/7 activation in IPEC-1 cells. DNA and protein oxidative damage triggered by AME were associated with an alteration of the antioxidant response, as shown by a decrease in the enzymatic activity of catalase and superoxide dismutase. These effects on the oxidative response can be related to an inhibition of the Akt/Nrf2/HO-1 signaling pathway; however, further studies are needed in order to validate these in vitro data using in vivo trials in swine.

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