CRISPR/Cas9-Based Functional Characterization of SfUGT50A15 Reveals Its Roles in the Resistance of to Chlorantraniliprole, Emamectin Benzoate, and Benzoxazinoids

Overview

Journal Insects

Specialty Biology

Date 2024 May 24

PMID 38786870

Authors

Zhan Shi

Mei Luo

Jinxi Yuan

Bin Gao

Minghuan Yang

Guirong Wang

Affiliations

Soon will be listed here.

Abstract

UDP-glycosyltransferases (UGTs) are a diverse superfamily of enzymes. Insects utilize uridine diphosphate-glucose (UDP-glucose) as a glycosyl donor for glycosylation in vivo, involved in the glycosylation of lipophilic endosymbionts and xenobiotics, including phytotoxins. UGTs act as second-stage detoxification metabolizing enzymes, which are essential for the detoxification metabolism of insecticides and benzoxazine compounds. However, the UGT genes responsible for specific glycosylation functions in are unclear at present. In this study, we utilized CRISPR/Cas9 to produce a strain to explore its possible function in governing sensitivity to chemical insecticides or benzoxazinoids. The bioassay results suggested that the strain was significantly more sensitive to chlorantraniliprole, emamectin benzoate, and benzoxazinoids than the wild-type strains. This finding suggests that the overexpression of the gene may be linked to resistance to pesticides (chlorantraniliprole and emamectin benzoate) as well as benzoxazinoids (BXDs).

Citing Articles

Physiological and Molecular Mechanisms of Lepidopteran Insects: Genomic Insights and Applications of Genome Editing for Future Research.

Niu D, Zhao Q, Xu L, Lin K Int J Mol Sci. 2024; 25(22).

PMID: 39596426 PMC: 11594828. DOI: 10.3390/ijms252212360.

References

Burchell B, Coughtrie M . UDP-glucuronosyltransferases. Pharmacol Ther. 1989; 43(2):261-89. DOI: 10.1016/0163-7258(89)90122-8. View

Heidel-Fischer H, Vogel H . Molecular mechanisms of insect adaptation to plant secondary compounds. Curr Opin Insect Sci. 2020; 8:8-14. DOI: 10.1016/j.cois.2015.02.004. View

Westbrook J, Nagoshi R, Meagher R, Fleischer S, Jairam S . Modeling seasonal migration of fall armyworm moths. Int J Biometeorol. 2015; 60(2):255-67. DOI: 10.1007/s00484-015-1022-x. View

Toni L, Garcia A, Jeffrey D, Jiang X, Stauffer B, Miyamoto S . Optimization of phenol-chloroform RNA extraction. MethodsX. 2018; 5:599-608. PMC: 6031757. DOI: 10.1016/j.mex.2018.05.011. View

Zhu G, Xu J, Cui Z, Dong X, Ye Z, Niu D . Functional characterization of SlitPBP3 in Spodoptera litura by CRISPR/Cas9 mediated genome editing. Insect Biochem Mol Biol. 2016; 75:1-9. DOI: 10.1016/j.ibmb.2016.05.006. View

Gui F, Lan T, Zhao Y, Guo W, Dong Y, Fang D . Genomic and transcriptomic analysis unveils population evolution and development of pesticide resistance in fall armyworm Spodoptera frugiperda. Protein Cell. 2020; 13(7):513-531. PMC: 9226219. DOI: 10.1007/s13238-020-00795-7. View

Pfaffl M . A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 2001; 29(9):e45. PMC: 55695. DOI: 10.1093/nar/29.9.e45. View

Wu K, Shirk P, Taylor C, Furlong R, Shirk B, Pinheiro D . CRISPR/Cas9 mediated knockout of the abdominal-A homeotic gene in fall armyworm moth (Spodoptera frugiperda). PLoS One. 2018; 13(12):e0208647. PMC: 6283638. DOI: 10.1371/journal.pone.0208647. View

Li X, Zhu B, Gao X, Liang P . Over-expression of UDP-glycosyltransferase gene UGT2B17 is involved in chlorantraniliprole resistance in Plutella xylostella (L.). Pest Manag Sci. 2016; 73(7):1402-1409. DOI: 10.1002/ps.4469. View

10.

Price M, Dehal P, Arkin A . FastTree: computing large minimum evolution trees with profiles instead of a distance matrix. Mol Biol Evol. 2009; 26(7):1641-50. PMC: 2693737. DOI: 10.1093/molbev/msp077. View

11.

Jin T, Lin Y, Chi H, Xiang K, Ma G, Peng Z . Comparative Performance of the Fall Armyworm (Lepidoptera: Noctuidae) Reared on Various Cereal-Based Artificial Diets. J Econ Entomol. 2020; 113(6):2986-2996. DOI: 10.1093/jee/toaa198. View

12.

Ahn S, Vogel H, Heckel D . Comparative analysis of the UDP-glycosyltransferase multigene family in insects. Insect Biochem Mol Biol. 2011; 42(2):133-47. DOI: 10.1016/j.ibmb.2011.11.006. View

13.

Esen A . Purification and Partial Characterization of Maize (Zea mays L.) beta-Glucosidase. Plant Physiol. 1992; 98(1):174-82. PMC: 1080166. DOI: 10.1104/pp.98.1.174. View

14.

Bozzolan F, Siaussat D, Maria A, Durand N, Pottier M, Chertemps T . Antennal uridine diphosphate (UDP)-glycosyltransferases in a pest insect: diversity and putative function in odorant and xenobiotics clearance. Insect Mol Biol. 2014; 23(5):539-49. DOI: 10.1111/imb.12100. View

15.

Dow J, Davies S . The Malpighian tubule: rapid insights from post-genomic biology. J Insect Physiol. 2005; 52(4):365-78. DOI: 10.1016/j.jinsphys.2005.10.007. View

16.

Su X, Li C, Zhang Y . Chlorpyrifos and chlorfenapyr resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae) relies on UDP-glucuronosyltransferases. J Econ Entomol. 2023; 116(4):1329-1341. DOI: 10.1093/jee/toad088. View

17.

Sun D, Zhu L, Guo L, Wang S, Wu Q, Crickmore N . A versatile contribution of both aminopeptidases N and ABC transporters to Bt Cry1Ac toxicity in the diamondback moth. BMC Biol. 2022; 20(1):33. PMC: 8817492. DOI: 10.1186/s12915-022-01226-1. View

18.

Agathokleous E . The hormetic response of heart rate of fish embryos to contaminants - Implications for research and policy. Sci Total Environ. 2022; 815:152911. DOI: 10.1016/j.scitotenv.2021.152911. View

19.

Yang Z, Xiao T, Lu K . Contribution of UDP-glycosyltransferases to chlorpyrifos resistance in Nilaparvata lugens. Pestic Biochem Physiol. 2023; 190:105321. DOI: 10.1016/j.pestbp.2022.105321. View

20.

Chen W, Hasegawa D, Kaur N, Kliot A, Pinheiro P, Luan J . The draft genome of whitefly Bemisia tabaci MEAM1, a global crop pest, provides novel insights into virus transmission, host adaptation, and insecticide resistance. BMC Biol. 2016; 14(1):110. PMC: 5157087. DOI: 10.1186/s12915-016-0321-y. View