LncRNA RPL29P2 Promotes Peritoneal Fibrosis and Impairs Peritoneal Transport Function Via MiR-1184 in Peritoneal Dialysis

Overview

Journal Int J Med Sci

Specialty General Medicine

Date 2024 May 22

PMID 38774747

Authors

Huan Li

Yuting Zhang

Mingwen Che

Hanmin Wang

Sutong Li

Peng He

Shiren Sun

Guoshuang Xu

Chen Huang

Xiaowei Liu

Ming Bai

Meilan Zhou

Binxiao Su

Peng Zhang

Lijie He

Affiliations

Soon will be listed here.

Abstract

Peritoneal dialysis (PD), hemodialysis and kidney transplantation are the three therapies to treat uremia. However, PD is discontinued for peritoneal membrane fibrosis (PMF) and loss of peritoneal transport function (PTF) due to damage from high concentrations of glucose in PD fluids (PDFs). The mechanism behind PMF is unclear, and there are no available biomarkers for the evaluation of PMF and PTF. Using microarray screening, we found that a new long noncoding RNA (lncRNA), RPL29P2, was upregulated in the PM (peritoneal membrane) of long-term PD patients, and its expression level was correlated with PMF severity and the PTF loss. and rat model assays suggested that lncRNA RPL29P2 targets miR-1184 and induces the expression of collagen type I alpha 1 chain (COL1A1). Silencing RPL29P2 in the PD rat model might suppress the HG-induced phenotypic transition of Human peritoneal mesothelial cells (HPMCs), alleviate HG-induced fibrosis and prevent the loss of PTF. Overall, our findings revealed that lncRNA RPL29P2, which targets miR-1184 and collagen, may represent a useful marker and therapeutic target of PMF in PD patients.

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