Establishment of Reference Values Based on Influential Characteristics of Hematopoietic Stem Cells and Immune Cell Subsets in the Bone Marrow

Overview

Journal Heliyon

Specialty Social Sciences

Date 2024 May 22

PMID 38774070

Authors

Rebar N Mohammed

Najmaddin S H Khoshnaw

Vian Faeq Mohammed

Dastan O Hassan

Chra Nawfal Abdullah

Tavan Ismael Mahmood

Huda A Abbass

Dereen Ahmed

Kani D Noori

Lanja I Saeed

Salah Mohammed Salih

Hiwa S Sidiq

Dlnya Omer Ali

Alan Shwan

Ignazio Majolino

Francesco Ipsevich

Affiliations

Soon will be listed here.

Abstract

Hematopoietic stem cell transplantation is still a curative treatment for many haematological cancers. Many factors, such as age, sex, ethnic background, smoking status, and body mass index, affect average reference values in different populations. This study aimed to establish a reference range for the absolute numbers and percentages of healthy individuals' hematopoietic stem cells and immune cells in the bone marrow. Seventy-one healthy donors (32 males and 39 females) were enrolled in the study. Following bone marrow harvesting, using flow cytometry, immunophenotyping was performed to determine the absolute number and percentage of CD34 stem cells and various immune subsets. We found no statistically significant difference in the absolute count of HSCs or immune cell subsets in the bone marrow between males and females. Regarding age, the younger group had more significant CD34 and immune cell subsets. Donors with healthier body weights tend to have richer bone marrow cellularity. Establishing a reference value for hematopoietic stem cells and immune cells in the bone marrow based on various influential factors is pivotal for defining bone marrow status and donor selection.

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