Microscopic and Molecular Prevalence and Associated Risk Factors with Toxocara and Blastocystis Infection in Dogs and Cats in Mitidja, Algeria

Overview

Journal Parasitol Res

Specialty Parasitology

Date 2024 May 21

PMID 38771352

Authors

Ikram Haleche

Asma Guilane

Amina Boutellis

Bachir Medrouh

Fairouz Saidi

Tahar Kernif

Hocine Ziam

Affiliations

Soon will be listed here.

Abstract

Domestic dogs and cats can serve as a source of environmental contamination with Toxocara spp. and Blastocystis spp., and this represents a neglected public and veterinary health problem. We assessed the microscopic and molecular prevalence of these species in a locality in Algeria and identified the associated risk factors. The faeces of 225 dogs and 78 cats were collected in Mitidja between March and July 2022. The samples were analysed by coproscopy and by polymerase chain reaction (PCR) targeting the Internal Transcribed Spacer 2 (ITS2) and Small Subunit Ribosomal (SSU-RNA) of T. canis and Blastocystis spp. respectively. The overall microscopic prevalence of Toxocara spp. in dogs and cats was 9.78 ± 1.98% and 12.82 ± 7.42%, respectively. The rate of Blastocystis spp. was 15.11 ± 2.39% and 15.38 ± 4.08% in dogs and cats, respectively while the molecular prevalence of T. canis in dogs was 4.89 ± 1.44% and in cats 1.28 ± 1.27%; the prevalence of Blastocystis spp. was 41.78 ± 3.29% and 34.62 ± 5.39% in dogs and cats, respectively. Phylogenetic and phylogeographic analyses identified the presence of the H1 subtype of T. canis in dogs, and the ST1 subtype of Blastocystis in dogs and cats. Dogs with clinical signs were more likely to be infected with T. canis (OR 6.039, P < 0.05) than healthy dogs. This study demonstrates that dogs and cats are carriers of Toxocara spp. and Blastocystis spp. and are therefore a source of environmental contamination. Veterinarians and human health professionals should work together to implement control strategies as part of a "One Health" approach to improving animal health and reducing the risk of transmission to humans.

References

Allen A, RIDLEY D . Further observations on the formol-ether concentration technique for faecal parasites. J Clin Pathol. 1970; 23(6):545-6. PMC: 476828. DOI: 10.1136/jcp.23.6.545. View

Abdel Aziz A, Hassan A, Elmahallawy E, Elshahawy I, Almuzaini A . Prevalence and associated risk factors of Toxocara infection in dogs in northern and southern Egypt. Vet Parasitol Reg Stud Reports. 2019; 17:100305. DOI: 10.1016/j.vprsr.2019.100305. View

Boutellis A, Aissi M, Harhoura K, Drali R, Kernif T, Tazerouti F . First molecular characterization of Blastocystis subtypes from animals and animal-keepers stool in Algeria. Comp Immunol Microbiol Infect Dis. 2021; 78:101695. DOI: 10.1016/j.cimid.2021.101695. View

Chidumayo N . Prevalence of Toxocara in dogs and cats in Africa. Adv Parasitol. 2020; 109:861-871. DOI: 10.1016/bs.apar.2020.01.032. View

Claerebout E, Casaert S, Dalemans A, De Wilde N, Levecke B, Vercruysse J . Giardia and other intestinal parasites in different dog populations in Northern Belgium. Vet Parasitol. 2009; 161(1-2):41-6. DOI: 10.1016/j.vetpar.2008.11.024. View

Fava N, Cury M, Santos H, Takeuchi-Storm N, Strube C, Zhu X . Phylogenetic relationships among Toxocara spp. and Toxascaris sp. from different regions of the world. Vet Parasitol. 2020; 282:109133. DOI: 10.1016/j.vetpar.2020.109133. View

Fogt-Wyrwas R, Mizgajska-Wiktor H, Pacon J, Jarosz W . Intraspecific variation between the ITS sequences of Toxocara canis, Toxocara cati and Toxascaris leonina from different host species in south-western Poland. J Helminthol. 2012; 87(4):432-42. DOI: 10.1017/S0022149X1200048X. View

Holland C, OConnor P, Taylor M, Hughes G, Girdwood R, Smith H . Families, parks, gardens and toxocariasis. Scand J Infect Dis. 1991; 23(2):225-31. DOI: 10.3109/00365549109023405. View

Hublin J, Maloney J, Santin M . Blastocystis in domesticated and wild mammals and birds. Res Vet Sci. 2020; 135:260-282. DOI: 10.1016/j.rvsc.2020.09.031. View

10.

Idrissi H, El Hamiani Khatat S, Duchateau L, Kachani M, Daminet S, El Asatey S . Prevalence, risk factors and zoonotic potential of intestinal parasites in dogs from four locations in Morocco. Vet Parasitol Reg Stud Reports. 2022; 34:100775. DOI: 10.1016/j.vprsr.2022.100775. View

11.

Jacobs D, Zhu X, Gasser R, Chilton N . PCR-based methods for identification of potentially zoonotic ascaridoid parasites of the dog, fox and cat. Acta Trop. 1997; 68(2):191-200. DOI: 10.1016/s0001-706x(97)00093-4. View

12.

Khademvatan S, Rahim F, Tavalla M, Abdizadeh R, Hashemitabar M . PCR-based molecular characterization of Toxocara spp. using feces of stray cats: a study from Southwest Iran. PLoS One. 2013; 8(6):e65293. PMC: 3670902. DOI: 10.1371/journal.pone.0065293. View

13.

Li M, Lin R, Chen H, Sani R, Song H, Zhu X . PCR tools for the verification of the specific identity of ascaridoid nematodes from dogs and cats. Mol Cell Probes. 2007; 21(5-6):349-54. DOI: 10.1016/j.mcp.2007.04.004. View

14.

Maloney J, Molokin A, Rodrigues da Cunha M, Cury M, Santin M . subtype distribution in domestic and captive wild bird species from Brazil using next generation amplicon sequencing. Parasite Epidemiol Control. 2020; 9:e00138. PMC: 6995250. DOI: 10.1016/j.parepi.2020.e00138. View

15.

McCain A, Gruneck L, Popluechai S, Tsaousis A, Gentekaki E . Circulation and colonisation of subtypes in schoolchildren of various ethnicities in rural northern Thailand. Epidemiol Infect. 2023; 151:e77. PMC: 10204141. DOI: 10.1017/S0950268823000596. View

16.

Oge H, Oge S, Ozbakis-Beceriklisoy G . Detection and Identification of in Infected Dogs Using PCR. Helminthologia. 2019; 56(2):118-123. PMC: 6799566. DOI: 10.2478/helm-2019-0008. View

17.

Overgaauw P . Aspects of Toxocara epidemiology: toxocarosis in dogs and cats. Crit Rev Microbiol. 1997; 23(3):233-51. DOI: 10.3109/10408419709115138. View

18.

Overgaauw P, van Knapen F . Veterinary and public health aspects of Toxocara spp. Vet Parasitol. 2013; 193(4):398-403. DOI: 10.1016/j.vetpar.2012.12.035. View

19.

Parija S, Jeremiah S . Blastocystis: Taxonomy, biology and virulence. Trop Parasitol. 2013; 3(1):17-25. PMC: 3745665. DOI: 10.4103/2229-5070.113894. View

20.

Paulos S, Koster P, de Lucio A, Hernandez-de-Mingo M, Cardona G, Fernandez-Crespo J . Occurrence and subtype distribution of Blastocystis sp. in humans, dogs and cats sharing household in northern Spain and assessment of zoonotic transmission risk. Zoonoses Public Health. 2018; 65(8):993-1002. DOI: 10.1111/zph.12522. View