In-Vitro Cytotoxicity, Apoptotic Property, and Gene Expression Changes Induced by Naringenin-7-O-Glucoside in Triple-Negative Breast Cancer

Overview

Journal Cureus

Specialty Biomedical Engineering

Date 2024 May 21

PMID 38770462

Authors

Akhila R James

Sakthidasan Jayaprakash

Lakshmi M Sundeep

Affiliations

Soon will be listed here.

Abstract

Introduction: Cancer is one of the most significant health challenges demanding the expansion of effectual therapeutic methods. Triple-negative breast cancer (TNBC) is a form of aggressive cancer with inadequate therapeutic options which lacks the expression of certain hormones.

Materials And Methods: The present study investigates the potential of naringenin-7-O-glucoside, a flavanone glycoside extracted from as an anticancer agent against TNBC cell lines. In-vitro analysis to evaluate cytotoxicity, apoptotic-inducing properties and effect on gene expression was conducted.

Results: MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay studied the IC-50 of naringenin-7-O-glucoside to be 233.56 µg/µL, revealing the dose-dependent cytotoxicity with minimal effect on Vero cells. Extensive DNA fragmentation confirmed the apoptotic property. Furthermore, a significant downregulation of the epidermal growth factor receptor (EGFR) was noted in treated cells when equated to the control specimen of the sample.

Conclusion: Therefore, naringenin-7-O-glucoside can be a potential targeted therapeutic agent.

Citing Articles

Morchella conica, Morchella esculenta and Morchella delicosa Induce Apoptosis in Breast and Colon Cancer Cell Lines via Pro-apoptotic and Anti-apoptotic Regulation.

Ul Haq F, Imran M, Ullah S, Aftab U, Akhtar T, Khan A Chin J Integr Med. 2024; .

PMID: 39225882 DOI: 10.1007/s11655-024-3819-0.

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