Cytotoxicity of Amine-modified Polystyrene MPs and NPs on Neural Stem Cells Cultured from Mouse Subventricular Zone

Overview

Journal Heliyon

Specialty Social Sciences

Date 2024 May 21

PMID 38770330

Authors

Ki-Youb Park

Man Su Kim

Nuri Oh

Affiliations

Soon will be listed here.

Abstract

Microplastics (MPs) and nanoplastics (NPs) are found in various environments such as aquatic, terrestrial, and aerial areas. Once ingested and inhaled, these tiny plastic debris damaged the digestive and respiratory organ systems in animals. In humans, the possible connection between MPs and various diseases such as lung diseases has been raised. Yet, the impact of MPs on the human nervous system has been unclear. Previous research using animals and cultured cells showed possible neurotoxicity of MPs and NPs. In this study, we used neural stem cells cultured from mouse subventricular zone to examine the effects of polystyrene (PS) NPs and MPs with sizes of 0.1 μm, 1 μm, and 2 μm on the cell proliferation and differentiation. We observed that only positively charged NPs and MPs, but not negatively charged ones, decreased cell viability and proliferation. These amine-modified NPs and MPs decreased both neurogenesis and oligodendrogenesis. Finally, fully differentiated neurons and oligodendrocytes were damaged and removed by the application of NPs and MPs. All these effects varied among different sizes of NPs and MPs, with the greatest effects from 1 μm and the least effects from 2 μm. These results clearly demonstrate the cytotoxicity and neurotoxicity of PS-NPs and MPs.

References

Hua T, Kiran S, Li Y, Sang Q . Microplastics exposure affects neural development of human pluripotent stem cell-derived cortical spheroids. J Hazard Mater. 2022; 435:128884. DOI: 10.1016/j.jhazmat.2022.128884. View

Dong C, Chen C, Chen Y, Chen H, Lee J, Lin C . Polystyrene microplastic particles: In vitro pulmonary toxicity assessment. J Hazard Mater. 2019; 385:121575. DOI: 10.1016/j.jhazmat.2019.121575. View

Obernier K, Alvarez-Buylla A . Neural stem cells: origin, heterogeneity and regulation in the adult mammalian brain. Development. 2019; 146(4). PMC: 6398449. DOI: 10.1242/dev.156059. View

Siddiqui S, Singh S, Bahmid N, Shyu D, Dominguez R, Lorenzo J . Polystyrene microplastic particles in the food chain: Characteristics and toxicity - A review. Sci Total Environ. 2023; 892:164531. DOI: 10.1016/j.scitotenv.2023.164531. View

Senathirajah K, Attwood S, Bhagwat G, Carbery M, Wilson S, Palanisami T . Estimation of the mass of microplastics ingested - A pivotal first step towards human health risk assessment. J Hazard Mater. 2020; 404(Pt B):124004. DOI: 10.1016/j.jhazmat.2020.124004. View

Park K, Kim S, Kim M . Effects of taxol on neuronal differentiation of postnatal neural stem cells cultured from mouse subventricular zone. Differentiation. 2021; 119:1-9. DOI: 10.1016/j.diff.2021.03.001. View

Xu S, Ma J, Ji R, Pan K, Miao A . Microplastics in aquatic environments: Occurrence, accumulation, and biological effects. Sci Total Environ. 2019; 703:134699. DOI: 10.1016/j.scitotenv.2019.134699. View

Kik K, Bukowska B, Sicinska P . Polystyrene nanoparticles: Sources, occurrence in the environment, distribution in tissues, accumulation and toxicity to various organisms. Environ Pollut. 2020; 262:114297. DOI: 10.1016/j.envpol.2020.114297. View

Chen Y, Chen K, Andrew Lin K, Chen J, Jiang X, Lin C . The nephrotoxic potential of polystyrene microplastics at realistic environmental concentrations. J Hazard Mater. 2021; 427:127871. DOI: 10.1016/j.jhazmat.2021.127871. View

10.

Bas O, Ilhan H, Hanci H, Celikkan H, Ekinci D, Degermenci M . To what extent are orally ingested nanoplastics toxic to the hippocampus in young adult rats?. J Chem Neuroanat. 2023; 132:102314. DOI: 10.1016/j.jchemneu.2023.102314. View

11.

Amato-Lourenco L, Carvalho-Oliveira R, Junior G, Dos Santos Galvao L, Ando R, Mauad T . Presence of airborne microplastics in human lung tissue. J Hazard Mater. 2021; 416:126124. DOI: 10.1016/j.jhazmat.2021.126124. View

12.

Lim S, Ng C, Zou L, Lu Y, Chen J, Bay B . Targeted metabolomics reveals differential biological effects of nanoplastics and nanoZnO in human lung cells. Nanotoxicology. 2019; 13(8):1117-1132. DOI: 10.1080/17435390.2019.1640913. View

13.

Chen S, Chen Y, Gao Y, Han B, Wang T, Dong H . Toxic effects and mechanisms of nanoplastics on embryonic brain development using brain organoids model. Sci Total Environ. 2023; 904:166913. DOI: 10.1016/j.scitotenv.2023.166913. View

14.

Lee C, Hsu L, Wu I, Wang Y, Chen W, Liu Y . Exposure to polystyrene microplastics impairs hippocampus-dependent learning and memory in mice. J Hazard Mater. 2022; 430:128431. DOI: 10.1016/j.jhazmat.2022.128431. View

15.

Shan S, Zhang Y, Zhao H, Zeng T, Zhao X . Polystyrene nanoplastics penetrate across the blood-brain barrier and induce activation of microglia in the brain of mice. Chemosphere. 2022; 298:134261. DOI: 10.1016/j.chemosphere.2022.134261. View

16.

Chen X, Xu L, Chen Q, Su S, Zhuang J, Qiao D . Polystyrene micro- and nanoparticles exposure induced anxiety-like behaviors, gut microbiota dysbiosis and metabolism disorder in adult mice. Ecotoxicol Environ Saf. 2023; 259:115000. DOI: 10.1016/j.ecoenv.2023.115000. View

17.

Zhao Y, Bao Z, Wan Z, Fu Z, Jin Y . Polystyrene microplastic exposure disturbs hepatic glycolipid metabolism at the physiological, biochemical, and transcriptomic levels in adult zebrafish. Sci Total Environ. 2020; 710:136279. DOI: 10.1016/j.scitotenv.2019.136279. View

18.

Roshanzadeh A, Park S, Ganjbakhsh S, Park J, Lee D, Lee S . Surface Charge-Dependent Cytotoxicity of Plastic Nanoparticles in Alveolar Cells under Cyclic Stretches. Nano Lett. 2020; 20(10):7168-7176. DOI: 10.1021/acs.nanolett.0c02463. View

19.

Wang S, Han Q, Wei Z, Wang Y, Xie J, Chen M . Polystyrene microplastics affect learning and memory in mice by inducing oxidative stress and decreasing the level of acetylcholine. Food Chem Toxicol. 2022; 162:112904. DOI: 10.1016/j.fct.2022.112904. View

20.

Lim D, Huang Y, Swigut T, Mirick A, Garcia-Verdugo J, Wysocka J . Chromatin remodelling factor Mll1 is essential for neurogenesis from postnatal neural stem cells. Nature. 2009; 458(7237):529-33. PMC: 3800116. DOI: 10.1038/nature07726. View