» Articles » PMID: 38769091

Downregulation of MiR-181c-5p in Alzheimer's Disease Weakens the Response of Microglia to Aβ Phagocytosis

Overview
Journal Sci Rep
Specialty Science
Date 2024 May 20
PMID 38769091
Authors
Affiliations
Soon will be listed here.
Abstract

Alzheimer's disease (AD) is an age-associated neurodegenerative disease. Recently, studies have demonstrated the potential involvement of microRNA-181c-5p (miR-181c-5p) in AD. However, the mechanism through which miR-181c-5p is responsible for the onset and progression of this disease remains unclear, and our study aimed to explore this problem. Differential expression analysis of the AD dataset was performed to identify dysregulated genes. Based on hypergeometric analysis, AD differential the upstream regulation genes miR-181c-5p was found. We constructed a model where SH-SY5Y and BV2 cells were exposed to Aβ1-42 to simulate AD. Levels of tumor necrosis factor-alpha, interleukin-6, and IL-1β were determined using enzyme-linked immunosorbent assay or reverse transcription quantitative polymerase chain reaction. Phosphorylation levels of p-P38 and P38 were detected by Western blot. The level of apoptosis in BV2 cells under Aβ1-42 stress was exacerbated by miR-181c-5p mimic. Downregulated miR-181c-5p impaired the phagocytosis and degradation of Aβ by BV2 cells. The release of proinflammatory cytokines in BV2 cells with Aβ1-42 stress was alleviated by miR-181c-5p upregulation. Additionally, miR-181c-5p downregulation alleviated the phosphorylation of P38 in Aβ1-42-induced SH-SY5Y cells. In conclusion, miR-181c-5p improves the phagocytosis of Aβ by microglial cells in AD patients, thereby reducing neuroinflammation.

References
1.
Qu C, Li Q, Su Z, Ip S, Yuan Q, Xie Y . Nano-Honokiol ameliorates the cognitive deficits in TgCRND8 mice of Alzheimer's disease via inhibiting neuropathology and modulating gut microbiota. J Adv Res. 2022; 35:231-243. PMC: 8721355. DOI: 10.1016/j.jare.2021.03.012. View

2.
Luo J, Chen L, Huang X, Xie J, Zou C, Pan M . REPS1 as a Potential Biomarker in Alzheimer's Disease and Vascular Dementia. Front Aging Neurosci. 2022; 14:894824. PMC: 9257827. DOI: 10.3389/fnagi.2022.894824. View

3.
Zhang J, Wang R . Deregulated lncRNA MAGI2-AS3 in Alzheimer's disease attenuates amyloid-β induced neurotoxicity and neuroinflammation by sponging miR-374b-5p. Exp Gerontol. 2020; 144:111180. DOI: 10.1016/j.exger.2020.111180. View

4.
English G, Spector S, Farr R, Carr R . Histopathology and immunofluorescent immunoglobulins in asthmatics with aspirin idiosyncrasy. Arch Otolaryngol Head Neck Surg. 1987; 113(4):377-9. DOI: 10.1001/archotol.1987.01860040039013. View

5.
Munoz-Castro C, Noori A, Magdamo C, Li Z, Marks J, Frosch M . Cyclic multiplex fluorescent immunohistochemistry and machine learning reveal distinct states of astrocytes and microglia in normal aging and Alzheimer's disease. J Neuroinflammation. 2022; 19(1):30. PMC: 8808995. DOI: 10.1186/s12974-022-02383-4. View