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Analysis of Meiotic Recombination in Drosophila Simulans Shows No Evidence of an Interchromosomal Effect

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Journal Genetics
Specialty Genetics
Date 2024 May 19
PMID 38762892
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Abstract

Chromosome inversions are of unique importance in the evolution of genomes and species because when heterozygous with a standard arrangement chromosome, they suppress meiotic crossovers within the inversion. In Drosophila species, heterozygous inversions also cause the interchromosomal effect, whereby the presence of a heterozygous inversion induces a dramatic increase in crossover frequencies in the remainder of the genome within a single meiosis. To date, the interchromosomal effect has been studied exclusively in species that also have high frequencies of inversions in wild populations. We took advantage of a recently developed approach for generating inversions in Drosophila simulans, a species that does not have inversions in wild populations, to ask if there is an interchromosomal effect. We used the existing chromosome 3R balancer and generated a new chromosome 2L balancer to assay for the interchromosomal effect genetically and cytologically. We found no evidence of an interchromosomal effect in D. simulans. To gain insights into the underlying mechanistic reasons, we qualitatively analyzed the relationship between meiotic double-stranded break (DSB) formation and synaptonemal complex (SC) assembly. We found that the SC is assembled prior to DSB formation as in D. melanogaster; however, we show that the SC is assembled prior to localization of the oocyte determination factor Orb, whereas in D. melanogaster, SC formation does not begin until the Orb is localized. Together, our data show no evidence that heterozygous inversions in D. simulans induce an interchromosomal effect and that there are differences in the developmental programming of the early stages of meiosis.

References
1.
Baudat F, Manova K, Yuen J, Jasin M, Keeney S . Chromosome synapsis defects and sexually dimorphic meiotic progression in mice lacking Spo11. Mol Cell. 2000; 6(5):989-98. DOI: 10.1016/s1097-2765(00)00098-8. View

2.
Stevison L, Hoehn K, Noor M . Effects of inversions on within- and between-species recombination and divergence. Genome Biol Evol. 2011; 3:830-41. PMC: 3171675. DOI: 10.1093/gbe/evr081. View

3.
Billmyre K, Cahoon C, Heenan G, Wesley E, Yu Z, Unruh J . chromosome and autosomal recombination are differentially sensitive to disruptions in SC maintenance. Proc Natl Acad Sci U S A. 2019; 116(43):21641-21650. PMC: 6815145. DOI: 10.1073/pnas.1910840116. View

4.
Hughes S, Miller D, Miller A, Hawley R . Female Meiosis: Synapsis, Recombination, and Segregation in . Genetics. 2018; 208(3):875-908. PMC: 5844340. DOI: 10.1534/genetics.117.300081. View

5.
Ye Q, Rosenberg S, Moeller A, Speir J, Su T, Corbett K . TRIP13 is a protein-remodeling AAA+ ATPase that catalyzes MAD2 conformation switching. Elife. 2015; 4. PMC: 4439613. DOI: 10.7554/eLife.07367. View