Visualizing the Spatial Distribution of Ustalic Acid in the Fruiting Body of Tricholoma Kakishimeji

Overview

Journal J Nat Med

Publisher Springer

Date 2024 May 18

PMID 38762660

Authors

Tetsuro Ito

Syu Taira

Wataru Aoki

Hiroyuki Nagai

Masashi Fukaya

Kaori Ryu

Akiyoshi Yamada

Affiliations

Soon will be listed here.

Abstract

Imaging mass spectrometry (IMS) was conducted for the first time using ustalic acid (UA) and the fruiting body of Tricholoma kakishimeji to localize mushroom toxins. The mushroom materials were systematically collected in Japan, and analysis of the cross sections of the materials at a resolution of 120 μm using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-IMS) revealed the localization of UA and its biogenically related metabolites. MALDI-IMS confirmed that UA was predominantly located on the entire surface of the fruiting body and accumulated in higher amounts in younger fruiting bodies than in mature ones. UA is the first toxic secondary metabolite in the genus Tricholoma locally identified using IMS in mushrooms.

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