Bioassay-guided Isolation of Three New Alkaloids from and Preliminary Mechanism Against Ginseng Root Rot

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2024 May 17

PMID 38756729

Authors

Miaomiao Xiong

Xiaomin Yang

Lan Yao

Zhuang Li

Jinxiu Zhang

Jianhua Lv

Affiliations

Soon will be listed here.

Abstract

In order to control the occurrence of ginseng root rot caused by (Mart.) Sacc., the antifungal compounds of the mushroom were investigated. And three new alkaloids (-), named bovinalkaloid A-C, along with one known analog (), were isolated and identified by bioassay-guided isolation and spectroscopic analyses. Compound strongly inhibited the mycelial growth and spore germination of with minimum inhibitory concentration of 2.08 mM. Increases in electrical conductivity, nucleic acid, and protein contents, and decreases in lipid content showed that the membrane permeability and integrity were damaged by compound . Compound also increased the contents of malondialdehyde and hydrogen peroxide and the activities of antioxidant enzymes, indicating that lipid peroxidation had taken place in . Compound may serve as a natural alternative to synthetic fungicides for the control of ginseng root rot.

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