Effect of Cholesterol-loaded Cyclodextrin Treatment on Boar Sperm Cryopreservation

Overview

Journal Anim Biosci

Specialty Veterinary Medicine

Date 2024 May 16

PMID 38754842

Authors

Silong Zhang

Hanbing Zhang

Kexiong Liu

Xiaoling Xu

Yusheng Qin

Linli Xiao

Chunmei Zhou

Jianliang Wu

Yan Liu

Jiahua Bai

Affiliations

Soon will be listed here.

Abstract

Objective: This study investigated the efficacy of different concentrations of cholesterolloaded cyclodextrin (CLC) on cryopreservation in boar sperm quality.

Methods: In this study, we treated boar sperm with different concentrations of CLC before freezing and analyzed the sperm cholesterol concentration, plasma membrane, acrosome integrity rate and total motility rate before and after freeze-thawing. We also investigated the levels of reactive oxygen species (ROS), malondialdehyde (MDA), adenosine triphosphate (ATP), and structural- and oxidative-damage related proteins in all groups after thawing.

Results: The results revealed that the cholesterol concentration of the CLC-treated groups was higher than that of the control group, both before freezing and after thawing (p<0.05). The plasma membrane integrity rate, acrosome integrity rate, and total motility rate of sperm were also enhanced after thawing in the CLC-treated group (all p<0.05). Moreover, ROS and MDA production and ATP loss were reduced in CLC-treated sperm during freezing and thawing (p<0.05). Finally, CLC pretreatment partially prevented the consumption of various proteins involved in metabolism including capping actin protein of muscle Z-line subunit beta (CAPZB), heat shock protein 90 alpha family class A member 1 (HSP90AA1) and phosphoglycerate mutase 2 (PGAM2) (p<0.05).

Conclusion: The CLC treatment increased cholesterol concentration and decreased structural injury and oxidative damage during boar sperm freezing and thawing, improving the efficacy of sperm cryopreservation in boar.

Citing Articles

Pyrroroquinoline Quinone (PQQ) Improves the Quality of Holstein Bull Semen during Cryopreservation.

Wang H, Liu K, Zeng W, Bai J, Xiao L, Qin Y Animals (Basel). 2024; 14(20).

PMID: 39457870 PMC: 11503688. DOI: 10.3390/ani14202940.

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