Immunoinformatics Studies and Design of a Novel Multi-epitope Peptide Vaccine Against Based on Calcium-dependent Protein Kinases Antigens Through an Analysis

Overview

Journal Clin Exp Vaccine Res

Date 2024 May 16

PMID 38752002

Authors

Ali Dalir Ghaffari

Fardin Rahimi

Affiliations

Soon will be listed here.

Abstract

Purpose: Infection by the intracellular apicomplexan parasite has serious clinical consequences in humans and veterinarians around the world. Although about a third of the world's population is infected with , there is still no effective vaccine against this disease. The aim of this study was to develop and evaluate a multimeric vaccine against using the proteins calcium-dependent protein kinase (CDPK)1, CDPK2, CDPK3, and CDPK5.

Materials And Methods: Top-ranked major histocompatibility complex (MHC)-I and MHC-II binding as well as shared, immunodominant linear B-cell epitopes were predicted and linked using appropriate linkers. Moreover, the 50S ribosomal protein L7/L12 (adjuvant) was mixed with the construct's N-terminal to increase the immunogenicity. Then, the vaccine's physicochemical characteristics, antigenicity, allergenicity, secondary and tertiary structure were predicted.

Results: The finally-engineered chimeric vaccine had a length of 680 amino acids with a molecular weight of 74.66 kDa. Analyses of immunogenicity, allergenicity, and multiple physiochemical parameters indicated that the constructed vaccine candidate was soluble, non-allergenic, and immunogenic, making it compatible with humans and hence, a potentially viable and safe vaccine candidate against parasite.

Conclusion: , the vaccine construct was able to trigger primary immune responses. However, further laboratory studies are needed to confirm its effectiveness and safety.

Citing Articles

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Majidiani H, Pourseif M, Kordi B, Sadeghi M, Najafi A BMC Infect Dis. 2024; 24(1):886.

PMID: 39210269 PMC: 11361240. DOI: 10.1186/s12879-024-09807-x.

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