Progresses in Overcoming the Limitations of in Vitro Erythropoiesis Using Human Induced Pluripotent Stem Cells

Overview

Journal Stem Cell Res Ther

Publisher Biomed Central

Date 2024 May 15

PMID 38750578

Authors

Hyeonwoo Ju

Yeowon Sohn

Yoojun Nam

Yeri Alice Rim

Affiliations

Soon will be listed here.

Abstract

Researchers have attempted to generate transfusable oxygen carriers to mitigate RBC supply shortages. In vitro generation of RBCs using stem cells such as hematopoietic stem and progenitor cells (HSPCs), embryonic stem cells (ESCs), and induced pluripotent stem cells (iPSCs) has shown promise. Specifically, the limited supplies of HSPCs and ethical issues with ESCs make iPSCs the most promising candidate for in vitro RBC generation. However, researchers have encountered some major challenges when using iPSCs to produce transfusable RBC products, such as enucleation and RBC maturation. In addition, it has proven difficult to manufacture these products on a large scale. In this review, we provide a brief overview of erythropoiesis and examine endeavors to recapitulate erythropoiesis in vitro using various cell sources. Furthermore, we explore the current obstacles and potential solutions aimed at enabling the large-scale production of transfusable RBCs in vitro.

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