Comparative Transcriptomic and Physiological Analyses Unravel Wheat Source Root Adaptation to Phosphorous Deficiency

Overview

Journal Sci Rep

Specialty Science

Date 2024 May 14

PMID 38745054

Authors

Daozhen Luo

Muhammad Usman

Fei Pang

Wenjie Zhang

Ying Qin

Qing Li

Yangrui Li

Yongxiu Xing

Dengfeng Dong

Affiliations

Soon will be listed here.

Abstract

Phosphorus (P) is a crucial macronutrient for plant growth and development. Basic metabolic processes regulate growth; however, the molecular detail of these pathways under low phosphorous (LP) in wheat is still unclear. This study aims to elucidate the varied regulatory pathways responses to LP stress in wheat genotypes. Phenotypic, physiological, and transcriptome analyses were conducted on Fielder (P efficient) and Ardito (P inefficient) wheat genotypes after four days of normal phosphorous (NP) and LP stress. In response to LP, Fielder outperformed Ardito, displaying higher chlorophyll content-SPAD values (13%), plant height (45%), stem diameter (12%), shoot dry weight (42%), and root biomass (75%). Root structure analysis revealed that Fielder had greater total root length (50%), surface area (56%), volume (15%), and diameter (4%) than Ardito under LP. These findings highlight Fielder's superior performance and adaptation to LP stress. Transcriptome analysis of wheat genotype roots identified 3029 differentially expressed genes (DEGs) in Fielder and 1430 in Ardito, highlighting LP-induced changes. Key DEGs include acid phosphatases (PAPs), phosphate transporters (PHT1 and PHO1), SPX, and transcription factors (MYB, bHLH, and WRKY). KEGG enrichment analysis revealed key pathways like plant hormones signal transduction, biosynthesis of secondary metabolites, and carbohydrate biosynthesis metabolism. This study unveils crucial genes and the intricate regulatory process in wheat's response to LP stress, offering genetic insights for enhancing plant P utilization efficiency.

Citing Articles

High-throughput root phenotyping and association analysis identified potential genomic regions for phosphorus use efficiency in wheat (Triticum aestivum L.).

Rajamanickam V, Sevanthi A, Swarbreck S, Gudi S, Singh N, Singh V Planta. 2024; 260(6):142.

PMID: 39557700 DOI: 10.1007/s00425-024-04577-x.

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