Antiseizure Activity of in the Pentylenetetrazol-Induced Seizure Model in Mice: Involvement of Flavonoids and Alkaloids

Overview

Journal Turk J Pharm Sci

Specialty Pharmacology

Date 2024 May 14

PMID 38742766

Authors

Relwende Justin Ouedraogo

Muhammad Jamal

Lassina Ouattara

Muhammad Nadeem-Ul-Haque

Faisal Khan

Shabana Usman Simjee

Georges Anicet Ouedraogo

Farzana Shaheen

Affiliations

Soon will be listed here.

Abstract

Objective: This study aimed to investigate whether Otto Kuntze organic and aqueous extracts are able to control seizures induced by pentylenetetrazol (PTZ) in mice based on flavonoid fingerprints and alkaloidal contents.

Materials And Methods: Ethanolic extract and decoction-derived fractions from roots, leaves, and stems were subjected to chromatographic fingerprinting using AlCl and screening for their antiseizure effects using PTZ-induced acute seizure model. From the fractions that showed potent bioactivities, plausible antiseizure alkaloids were isolated using thin layer chromatography, and their structures were elucidated using H NMR, 2D NMR, C NMR, and FAB-HR ( or ).

Results: All fractions, with the exception of the dichloromethane and hexane fractions, revealed remarkable flavonoid fingerprints. An acute PTZ-induced seizure test revealed that ethanolic extract of stem bark [500 mg/kg body weight (bw)], ethyl acetate extract of stem bark (500 mg/kg bw), and aqueous extract of leaves (300 mg/kg bw) significantly delayed the occurrence of hind limb tonic extension (HLTE); however, a non-significant delay was observed in the onset of first myoclonic jerk compared with control animals. Isolation yielded four main alkaloids: that are, pteropodine (1), isopteropodine (2), mitraphylline (3) and corynoxeine (4). Corynoxeine is a new compound derived from .

Conclusion: This study suggests that flavonoid fingerprints are tracers of anticonvulsant ingredients. The stem bark ethanolic and ethyl acetate extracts and leaf aqueous extracts contain anticonvulsant bioactive principles that delay notifying the HLTE occurring in male naval medical research institute mice. Furthermore, alkaloidal contents also remain plausible bioactive anticonvulsant principles. All observations support the traditional use of to manage epilepsy. However, further studies are needed to understand the effects of alkaloid fractions, flavonoids, and the isolated compounds as promising antiseizure agents derived from in experimental animals.

Citing Articles

Saccharomyces Boulardii alleviates neuroinflammation and oxidative stress in PTZ-kindled seizure rat model.

Mirzababaei M, Babaei F, Ghafghazi S, Rahimi Z, Asadi S, Dargahi L Naunyn Schmiedebergs Arch Pharmacol. 2024; 398(2):1625-1635.

PMID: 39141021 DOI: 10.1007/s00210-024-03361-8.

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