MSC Microvesicles Loaded G-quadruplex-enhanced Circular Single-stranded DNA-9 Inhibits Tumor Growth by Targeting MDSCs

Overview

Journal J Nanobiotechnology

Publisher Biomed Central

Specialty Biotechnology

Date 2024 May 12

PMID 38735920

Authors

Jingxia Han

Rong Qin

Shaoting Zheng

Xiaohui Hou

Xiaorui Wang

Huihui An

Zhongwei Li

Yinan Li

Heng Zhang

Denghui Zhai

Huijuan Liu

Jing Meng

Tao Sun

Affiliations

Soon will be listed here.

Abstract

Background: Myeloid-derived suppressor cells (MDSCs) promote tumor growth, metastasis, and lead to immunotherapy resistance. Studies revealed that miRNAs are also expressed in MDSCs and promote the immunosuppressive function of MDSCs. Currently, few studies have been reported on inducible cellular microvesicle delivery of nucleic acid drugs targeting miRNA in MDSCs for the treatment of malignant tumors.

Results And Conclusion: In this study, we designed an artificial DNA named G-quadruplex-enhanced circular single-stranded DNA-9 (G4-CSSD9), that specifically adsorbs the miR-9 sequence. Its advanced DNA folding structure, rich in tandem repeat guanine (G-quadruplex), also provides good stability. Mesenchymal stem cells (MSCs) were prepared into nanostructured vesicles by membrane extrusion. The MSC microvesicles-encapsulated G4-CSSD9 (MVs@G4-CSSD9) was delivered into MDSCs, which affected the downstream transcription and translation process, and reduced the immunosuppressive function of MDSCs, so as to achieve the purpose of treating melanoma. In particular, it provides an idea for the malignant tumor treatment.

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PMID: 39023414 PMC: 11256889. DOI: 10.1002/iid3.1351.

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