Temperature Effects on Expression Levels of Genes in Eggs and Second-Stage Juveniles of Chitwood, 1949

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2024 May 11

PMID 38732085

Authors

Lukasz Flis

Tadeusz Malewski

Renata Dobosz

Affiliations

Soon will be listed here.

Abstract

is one of the most important nematode pathogens. It is a sedentary, biotrophic parasite of plants that overwinters in the soil or in diseased roots. The development of is temperature dependent. Numerous studies have been performed on the effect of temperature on the development of , but only a few of them analyzed the heat shock protein () genes. The aim of the study was to perform expression profiling of eight genes (90, 1, 4, 6, 60, 19, 43, and 12.2) at two development stages of , i.e., in eggs and second-stage juveniles (J2). The eggs and J2 were incubated under cold stress (5 °C), heat stress (35 °C, 40 °C), and non-stress (10 °C, 20 °C, and 30 °C) conditions. Expression profiling was performed by qPCR. It was demonstrated that only two genes, 60 and 19, have been upregulated by heat and cold stress at both development stages. Heat stress upregulated the expression of more genes than cold stress did. The level of upregulation of most genes was more marked in J2 than in eggs. The obtained results suggest that the 90 and 1 genes can be used as bioindicators of environmental impacts on nematodes of the genus.

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