MdbZIP44-MdCPRF2-like- Regulate Starch and Sugar Metabolism in Apple Under Nitrogen Supply

Overview

Journal Hortic Res

Publisher Oxford University Press

Date 2024 May 10

PMID 38725457

Authors

Xuejing Cao

Zhigang Guo

Ping Wang

Shixiong Lu

Wenfang Li

Zonghuan Ma

Juan Mao

Baihong Chen

Affiliations

Soon will be listed here.

Abstract

Nitrogen (N) is regarded as an essential macronutrient and is tightly associated with carbon (C) metabolism in plants. The transcriptome data obtained from this study showed that the expression level of the apple basic leucine zipper (bZIP) transcription factor (TF) was up-regulated in 'Oregon Spur Delicious' ( Borkh.) apple fruits under nitrogen supply. MdbZIP44 bound to the promoter of gene and inhibited its expression, thereby promoting starch accumulation and decreasing glucose content in apple and tomato fruits. Besides, overexpression of promoted sucrose accumulation by regulating the activities of sucrose metabolism-related enzymes and the expression of sugar metabolism-related genes in apple callus and tomato fruits. Furthermore, biochemical assays indicated that MdbZIP44 directly interacted with MdCPRF2-like, another bZIP gene in apple. Meanwhile, this study found that MdCPRF2-like, along with the MdbZIP44 and MdCPRF2-like complex, could activate the expression of , respectively. In conclusion, this study provides a new reference for potential mechanisms underlying that MdbZIP44-MdCPRF2-like- regulates starch and sugar metabolism under nitrogen supply.

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