T-lymphocyte Subpopulations in the Peripheral Blood and Bone Marrow of Patients with Aplastic Anemia

Overview

Journal Blut

Specialty Hematology

Date 1985 Feb 1

PMID 3871647

Citations 3

Authors

R P Falcao

J C Voltarelli

C Bottura

Affiliations

Soon will be listed here.

Abstract

A decrease in the absolute number of total lymphocytes, OKT3+ and OKT4+ lymphocytes, and a normal number of OKT8+ lymphocytes were found in the peripheral blood of patients with aplastic anemia. The OKT4:OKT8 ratio was decreased in patients due to a reduction in the percentage of OKT4+ cells and 3 out of 18 patients had a ratio less than 1. The values of the OKT4:OKT8 ratio were not associated either with the severity of the disease or with treatment with androgens. There was no correlation between the OKT4:OKT8 ratio and the number of transfusions received by patients. On the other hand, studies performed with bone marrow lymphocytes showed that the OKT4:OKT8 ratio for both patients and controls was lower than that of the peripheral blood. Since the ratio of OKT4:OKT8 cells in aplastic and control bone marrow was similar no direct pathogenic role can be assigned to the marrow for the imbalance detected in the peripheral blood.

Citing Articles

Overproduction of inhibitory hematopoietic cytokines by lipopolysaccharide-activated peripheral blood mononuclear cells in patients with aplastic anemia.

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PMID: 8534759 DOI: 10.1007/BF01697980.

Lymphocyte subpopulations in solvent-exposed workers.

Denkhaus W, von Steldern D, Botzenhardt U, KONIETZKO H Int Arch Occup Environ Health. 1986; 57(2):109-15.

PMID: 3949394 DOI: 10.1007/BF00381378.

Identification of activated T cells and the suppressor/inducer subset in patients suffering from severe aplastic anemia.

Koller U, Hinterberger W, Gschwandtler L, Grumayer R, Majdic O, Knapp W Blut. 1989; 58(1):21-6.

PMID: 2521803 DOI: 10.1007/BF00320231.

References

Bacigalupo A, Podesta M, Mingari M, Moretta L, Van Lint M, Marmont A . Immune suppression of hematopoiesis in aplastic anemia: activity of T-gamma lymphocytes. J Immunol. 1980; 125(4):1449-53. View

Falcao R, Voltarelli J, Bottura C . Some immunological studies in aplastic anaemia. J Clin Lab Immunol. 1983; 10(1):25-8. View

Mangan K, Chikkappa G, Bieler L, SCHARFMAN W, Parkinson D . Regulation of human blood erythroid burst-forming unit (BFU-E) proliferation by T-lymphocyte subpopulations defined by Fc receptors and monoclonal antibodies. Blood. 1982; 59(5):990-6. View

Sabbe L, Haak H, te Velde J, Bradley B, De Bode L, Blom J . Immunological investigations in aplastic anemia patients. Acta Haematol. 1984; 71(3):178-88. DOI: 10.1159/000206582. View

Voltarelli J, Falcao R, Carvalho I, Bottura C . K-cell activity in aplastic anaemia. Scand J Haematol. 1983; 30(5):451-7. DOI: 10.1111/j.1600-0609.1983.tb02533.x. View

Barr R, Stevens C . The role of autologous helper and suppressor T cells in the regulation of human granulopoiesis. Am J Hematol. 1982; 12(4):323-6. DOI: 10.1002/ajh.2830120403. View

Kuriyama K, Tomonaga M, Jinnai I, Matsuo T, Yoshida Y, Amenomori T . Reduced helper (OKT4+): suppressor (OKT8+) T ratios in aplastic anaemia: relation to immunosuppressive therapy. Br J Haematol. 1984; 57(2):329-36. DOI: 10.1111/j.1365-2141.1984.tb02902.x. View

Livnat S, Seigneuret M, Storb R, Prentice R . Analysis of cytotoxic effector cell function in patients with leukemia or aplastic anemia before and after marrow transplantation. J Immunol. 1980; 124(1):481-90. View

Bagby Jr G . T lymphocytes involved in inhibition of granulopoiesis in two neutropenic patients are of the cytotoxic/suppressor (T3+T8+) subset. J Clin Invest. 1981; 68(6):1597-600. PMC: 370965. DOI: 10.1172/jci110415. View

10.

Foon K, Mitsuyasu R, Schroff R, McIntyre R, Champlin R, Gale R . Immunologic defects in young male patients with hepatitis-associated aplastic anemia. Ann Intern Med. 1984; 100(5):657-62. DOI: 10.7326/0003-4819-100-5-657. View

11.

Ruiz-Arguelles G, Katzmann J, Greipp P, Marin-Lopez A . Lymphocyte subsets in patients with aplastic anemia. Am J Hematol. 1984; 16(3):267-75. DOI: 10.1002/ajh.2830160308. View

12.

Elfenbein G, Kallman C, Tutschka P, Adkinson Jr N, Bias W, Braine H . The immune system in 40 aplastic anemia patients receiving conventional therapy. Blood. 1979; 53(4):652-65. View

13.

Falcao R, Voltarelli J, Bottura C . T-cell subsets in patients with aplastic anemia. Braz J Med Biol Res. 1984; 17(2):151-6. View

14.

Torok-Storb B, Martin P, Hansen J . Regulation of in vitro erythropoiesis by normal T cells: evidence for two T-cell subsets with opposing function. Blood. 1981; 58(1):171-4. View

15.

Reinherz E, Moretta L, Roper M, Breard J, Mingari M, Cooper M . Human T lymphocyte subpopulations defined by Fc receptors and monoclonal antibodies. A comparison. J Exp Med. 1980; 151(4):969-74. PMC: 2185839. DOI: 10.1084/jem.151.4.969. View

16.

Nagasawa T, Abe T, Hanada T . Inhibitory effects of T cells on in vitro granulopoiesis, erythropoiesis, and immunoglobulin production in patients with aplastic anaemia. Scand J Haematol. 1982; 28(5):389-98. DOI: 10.1111/j.1600-0609.1982.tb00544.x. View

17.

Janossy G, Tidman N, Papageorgiou E, Kung P, Goldstein G . Distribution of t lymphocyte subsets in the human bone marrow and thymus: an analysis with monoclonal antibodies. J Immunol. 1981; 126(4):1608-13. View

18.

Bacigalupo A, Podesta M, Mingari M, Moretta L, Piaggio G, Van Lint M . Generation of CFU-C/suppressor T cells in vitro: an experimental model for immune-mediated marrow failure. Blood. 1981; 57(3):491-6. View