Protective Effects of the Bioactive Peptide from Maggots Against Skin Flap Ischemia‒reperfusion Injury in Rats

Overview

Journal Heliyon

Specialty Social Sciences

Date 2024 May 2

PMID 38694094

Authors

Hao Chen

Tianqi Zhang

Su Yan

Shan Zhang

Qiuyue Fu

Chuchu Xiong

Lina Zhou

Xiao Ma

Rong Wang

Gang Chen

Affiliations

Soon will be listed here.

Abstract

Ischemia‒reperfusion (I/R) injury is a frequently observed complication after flap surgery, and it affects skin flap survival and patient prognosis. Currently, there are no proven safe and effective treatment options to treat skin flap I/R injury. Herein, the potential efficacies of the bioactive peptide from maggots (BPM), as well as its underlying mechanisms, were explored in a rat model of skin flap I/R injury and LPS- or HO-elicited RAW 264.7 cells. We demonstrated that BPM significantly ameliorated the area of flap survival, and histological changes in skin tissue Furthermore, BPM could markedly restore or enhance Nrf2 and HO-1 levels, and suppress the expression of pro-inflammatory cytokines, including TLR4, -IκB, NFκB p65, p-p65, IL-6, and TNF-α in I/R-injured skin flaps. In addition, BPM treatment exhibited excellent biocompatibility with an adequate safety profile, while it exhibited superior ROS-scavenging ability and the upregulation of antioxidant enzymes . Mechanistically, the above benefits related to BPM involved the activation of Nrf2/HO-1 and suppression of TLR4/NF-κB pathway. Taken together, this study may provide a scientific basis for the potential therapeutic effect of BPM in the prevention of skin flap I/R injury and other related diseases.

Citing Articles

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PMID: 39821658 DOI: 10.1007/s00403-024-03696-5.

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