Pulmonary Mucus Hydration Assay Using Rotational and Translational Diffusion of Gold Nanorods with Polarization-sensitive Optical Coherence Tomography

Overview

Journal J Biomed Opt

Specialties Biomedical Engineering
Ophthalmology

Date 2024 May 1

PMID 38690122

Authors

Kelsey J Oeler

Richard L Blackmon

Silvia M Kreda

Taylor Robinson

Melanie Ghelardini

Brian S Chapman

Joseph Tracy

David B Hill

Amy L Oldenburg

Affiliations

Soon will be listed here.

Abstract

Significance: Assessing the nanostructure of polymer solutions and biofluids is broadly useful for understanding drug delivery and disease progression and for monitoring therapy.

Aim: Our objective is to quantify bronchial mucus solids concentration (wt. %) during hypertonic saline (HTS) treatment via nanostructurally constrained diffusion of gold nanorods (GNRs) monitored by polarization-sensitive optical coherence tomography (PS-OCT).

Approach: Using PS-OCT, we quantified GNR translational () and rotational () diffusion coefficients within polyethylene oxide solutions (0 to 3 wt. %) and human bronchial epithelial cell (hBEC) mucus (0 to 6.4 wt. %). Interpolation of and data is used to develop an assay to quantify mucus concentration. The assay is demonstrated on the mucus layer of an air-liquid interface hBEC culture during HTS treatment.

Results: In polymer solutions and mucus, and monotonically decrease with increasing concentration. is more sensitive than to changes above 1.5 wt. % of mucus and exhibits less intrasample variability. Mucus on HTS-treated hBEC cultures exhibits dynamic mixing from cilia. A region of hard-packed mucus is revealed by measurements.

Conclusions: The extended dynamic range afforded by simultaneous measurement of and of GNRs using PS-OCT enables resolving concentration of the bronchial mucus layer over a range from healthy to disease in depth and time during HTS treatment .

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