Detection of SARS-CoV-2 RNA in a Zoo-Kept Red Fox ()

Overview

Journal Viruses

Publisher MDPI

Specialty Microbiology

Date 2024 Apr 27

PMID 38675864

Authors

Tatjana Chan

Julia Ginders

Evelyn Kuhlmeier

Marina L Meli

Eva Bonzli

Theres Meili

Julia Huttl

Jean-Michel Hatt

Karin Hindenlang Clerc

Anja Kipar

Fabia Wyss

Christian Wenker

Marie-Pierre Ryser-Degiorgis

Cecilia Valenzuela Agui

Christian Urban

Christian Beisel

Tanja Stadler

Regina Hofmann-Lehmann

Affiliations

Soon will be listed here.

Abstract

Many different animal species are susceptible to SARS-CoV-2, including a few Canidae (domestic dog and raccoon dog). So far, only experimental evidence is available concerning SARS-CoV-2 infections in red foxes (). This is the first report of SARS-CoV-2 RNA detection in a sample from a red fox. The RT-qPCR-positive fox was zoo-kept together with another fox and two bears in the Swiss Canton of Zurich. Combined material from a conjunctival and nasal swab collected for canine distemper virus diagnostics tested positive for SARS-CoV-2 RNA with Ct values of 36.9 (E gene assay) and 35.7 (RdRp gene assay). The sample was analysed for SARS-CoV-2 within a research project testing residual routine diagnostic samples from different animal species submitted between spring 2020 and December 2022 to improve knowledge on SARS-CoV-2 infections within different animal species and investigate their potential role in a One Health context. Within this project, 246 samples from 153 different animals from Swiss zoos and other wild animal species all tested SARS-CoV-2 RT-qPCR and/or serologically negative so far, except for the reported fox. The source of SARS-CoV-2 in the fox is unknown. The fox disappeared within the naturally structured enclosure, and the cadaver was not found. No further control measures were undertaken.

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References

Fernandez-Bellon H, Rodon J, Fernandez-Bastit L, Almagro V, Padilla-Sole P, Lorca-Oro C . Monitoring Natural SARS-CoV-2 Infection in Lions () at the Barcelona Zoo: Viral Dynamics and Host Responses. Viruses. 2021; 13(9). PMC: 8472846. DOI: 10.3390/v13091683. View

Fang R, Yang X, Guo Y, Peng B, Dong R, Li S . SARS-CoV-2 infection in animals: Patterns, transmission routes, and drivers. Eco Environ Health. 2024; 3(1):45-54. PMC: 10758742. DOI: 10.1016/j.eehl.2023.09.004. View

Klaus J, Zini E, Hartmann K, Egberink H, Kipar A, Bergmann M . SARS-CoV-2 Infection in Dogs and Cats from Southern Germany and Northern Italy during the First Wave of the COVID-19 Pandemic. Viruses. 2021; 13(8). PMC: 8402904. DOI: 10.3390/v13081453. View

Nederlof R, de la Garza M, Bakker J . Perspectives on SARS-CoV-2 Cases in Zoological Institutions. Vet Sci. 2024; 11(2). PMC: 10893009. DOI: 10.3390/vetsci11020078. View

Kant R, Kareinen L, Smura T, Freitag T, Kumar Jha S, Alitalo K . Common Laboratory Mice Are Susceptible to Infection with the SARS-CoV-2 Beta Variant. Viruses. 2021; 13(11). PMC: 8619350. DOI: 10.3390/v13112263. View

Kuhlmeier E, Chan T, Valenzuela Agui C, Willi B, Wolfensberger A, Beisel C . Detection and Molecular Characterization of the SARS-CoV-2 Delta Variant and the Specific Immune Response in Companion Animals in Switzerland. Viruses. 2023; 15(1). PMC: 9864232. DOI: 10.3390/v15010245. View

Jemersic L, Lojkic I, Kresic N, Keros T, Amsel Zelenika T, Jurinovic L . Investigating the Presence of SARS CoV-2 in Free-Living and Captive Animals. Pathogens. 2021; 10(6). PMC: 8224323. DOI: 10.3390/pathogens10060635. View

McAloose D, Laverack M, Wang L, Killian M, Caserta L, Yuan F . From People to : Natural SARS-CoV-2 Infection in Tigers and Lions at the Bronx Zoo. mBio. 2020; 11(5). PMC: 7554670. DOI: 10.1128/mBio.02220-20. View

Wernike K, Aebischer A, Michelitsch A, Hoffmann D, Freuling C, Balkema-Buschmann A . Multi-species ELISA for the detection of antibodies against SARS-CoV-2 in animals. Transbound Emerg Dis. 2020; 68(4):1779-1785. PMC: 7753575. DOI: 10.1111/tbed.13926. View

10.

Posada-Cespedes S, Seifert D, Topolsky I, Jablonski K, Metzner K, Beerenwinkel N . V-pipe: a computational pipeline for assessing viral genetic diversity from high-throughput data. Bioinformatics. 2021; 37(12):1673-1680. PMC: 8289377. DOI: 10.1093/bioinformatics/btab015. View

11.

Aguilo-Gisbert J, Padilla-Blanco M, Lizana V, Maiques E, Munoz-Baquero M, Chillida-Martinez E . First Description of SARS-CoV-2 Infection in Two Feral American Mink () Caught in the Wild. Animals (Basel). 2021; 11(5). PMC: 8156136. DOI: 10.3390/ani11051422. View

12.

Porter S, Hartwig A, Bielefeldt-Ohmann H, Bosco-Lauth A, Root J . Susceptibility of Wild Canids to SARS-CoV-2. Emerg Infect Dis. 2022; 28(9):1852-1855. PMC: 9423904. DOI: 10.3201/eid2809.220223. View

13.

Nadeau S, Vaughan T, Beckmann C, Topolsky I, Chen C, Hodcroft E . Swiss public health measures associated with reduced SARS-CoV-2 transmission using genome data. Sci Transl Med. 2022; 15(680):eabn7979. PMC: 9765449. DOI: 10.1126/scitranslmed.abn7979. View

14.

Klaus J, Meli M, Willi B, Nadeau S, Beisel C, Stadler T . Detection and Genome Sequencing of SARS-CoV-2 in a Domestic Cat with Respiratory Signs in Switzerland. Viruses. 2021; 13(3). PMC: 8002591. DOI: 10.3390/v13030496. View

15.

Padilla-Blanco M, Aguilo-Gisbert J, Rubio V, Lizana V, Chillida-Martinez E, Cardells J . The Finding of the Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV-2) in a Wild Eurasian River Otter () Highlights the Need for Viral Surveillance in Wild Mustelids. Front Vet Sci. 2022; 9:826991. PMC: 9010019. DOI: 10.3389/fvets.2022.826991. View

16.

Koeppel K, Mendes A, Strydom A, Rotherham L, Mulumba M, Venter M . SARS-CoV-2 Reverse Zoonoses to Pumas and Lions, South Africa. Viruses. 2022; 14(1). PMC: 8778549. DOI: 10.3390/v14010120. View

17.

Deng J, Jin Y, Liu Y, Sun J, Hao L, Bai J . Serological survey of SARS-CoV-2 for experimental, domestic, companion and wild animals excludes intermediate hosts of 35 different species of animals. Transbound Emerg Dis. 2020; 67(4):1745-1749. PMC: 7264586. DOI: 10.1111/tbed.13577. View