Development of CuO Nanoparticles from the Mucus of Garden Snail As New Antimicrobial Agents

Overview

Journal Pharmaceuticals (Basel)

Publisher MDPI

Specialty Chemistry

Date 2024 Apr 27

PMID 38675466

Authors

Pavlina Dolashka

Karina Marinova

Petar Petrov

Ventsislava Petrova

Bogdan Ranguelov

Stella Atanasova-Vladimirova

Dimitar Kaynarov

Ivanka Stoycheva

Emiliya Pisareva

Anna Tomova

Angelina Kosateva

Lyudmila Velkova

Aleksandar Dolashki

Affiliations

Soon will be listed here.

Abstract

Several biologically active compounds involved in the green synthesis of silver and gold nanoparticles have been isolated from snail mucus and characterized. This paper presents a successful method for the application of snail mucus from as a bioreducing agent of copper sulfate and as a biostabilizer of the copper oxide nanoparticles (CuONPs-Muc) obtained. The synthesis at room temperature and neutral pH yielded nanoparticles with a spherical shape and an average diameter of 150 nm. The structure and properties of CuONPs-Muc were characterized using various methods and techniques, such as ultraviolet-visible spectroscopy (UV-vis), high-performance liquid chromatography (HPLC), one-dimensional polyacrylamide gel electrophoresis (1D-PAGE), up-conversion infrared spectroscopy Fourier transform (FTIR), scanning electron microscopy combined with energy dispersive spectroscopy (SEM/EDS), Raman spectroscopy and imaging, thermogravimetric analysis (TG-DSC), etc. Mucus proteins with molecular weights of 30.691 kDa and 26.549 kDa were identified, which are involved in the biogenic production of CuONPs-Muc. The macromolecular shell of proteins formed around the copper ions contributes to a higher efficiency of the synthesized CuONPs-Muc in inhibiting the bacterial growth of several Gram-positive (Bacillus subtilis NBIMCC2353, ATCC 6633, ATCC 6538, NBIMCC8755) and Gram-negative ( ATCC8739, NBIMCC8691, ATCC 14028, ATCC 17666) bacteria compared to baseline mucus. The bioorganic synthesis of snail mucus presented here provides CuONPs-Muc with a highly pronounced antimicrobial effect. These results will expand knowledge in the field of natural nanomaterials and their role in emerging dosage forms.

Citing Articles

The Role of Oxidative Stress in the Antifungal Activity of Two Mollusk Fractions on Resistant Fungal Strains.

Velkova L, Abrashev R, Miteva-Staleva J, Dishliyska V, Dolashki A, Spasova B Int J Mol Sci. 2025; 26(3).

PMID: 39940751 PMC: 11817555. DOI: 10.3390/ijms26030985.

Green Synthesis of Antibacterial CuO Nanoparticles Based on the Synergy Between Snail Mucus and Ascorbic Acid.

Todorova M, Kosateva A, Petrova V, Ranguelov B, Atanasova-Vladimirova S, Avdeev G Molecules. 2025; 30(2).

PMID: 39860160 PMC: 11768038. DOI: 10.3390/molecules30020291.

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