Multi-Omics Approaches Provide New Insights into the Identification of Putative Fungal Effectors from

Overview

Journal Microorganisms

Specialty Microbiology

Date 2024 Apr 27

PMID 38674600

Authors

Gulnaz Kahar

Yakupjan Haxim

Abdul Waheed

Tohir A Bozorov

Xiaojie Liu

Xuejing Wen

Mingqi Zhao

Daoyuan Zhang

Affiliations

Soon will be listed here.

Abstract

Pathogenic fungi secrete numerous effectors into host cells to manipulate plants' defense mechanisms. , a necrotrophic fungus, severely impacts apple production in China due to the occurrence of Valsa canker. Here, we predicted 210 candidate effector protein (CEP)-encoding genes from . The transcriptome analysis revealed that 146 CEP-encoding genes were differentially expressed during the infection of the host, . Proteome analysis showed that 27 CEPs were differentially regulated during the infection stages. Overall, 25 of the 146 differentially expressed CEP-encoding genes were randomly selected to be transiently expressed in . Pathogenicity analysis showed that the transient expression of VM1G-05058 suppressed BAX-triggered cell death while the expression of VM1G-10148 and VM1G-00140 caused cell death in . In conclusion, by using multi-omics analysis, we identified potential effector candidates for further evaluation in vivo. Our results will provide new insights into the investigation of virulent mechanisms of .

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