Identification of Chromosomal Regions and Candidate Genes for Locus in L

Overview

Journal Plants (Basel)

Date 2024 Apr 27

PMID 38674543

Authors

Xufeng Fang

Zicheng Zhu

Junyan Li

Xuezheng Wang

Chunhua Wei

Xian Zhang

Zuyun Dai

Shi Liu

Feishi Luan

Affiliations

Soon will be listed here.

Abstract

Leaf morphology plays a crucial role in plant classification and provides a significant model for studying plant diversity while directly impacting photosynthetic efficiency. In the case of melons, leaf shape not only influences production and classification but also represents a key genetic trait that requires further exploration. In this study, we utilized forward genetics to pinpoint a recessive locus, dubbed (), which is responsible for regulating melon leaf shape. Through bulked segregant analysis sequencing and extensive evaluation of a two-year F population, we successfully mapped the locus to a 537.07 kb region on chromosome 8 of the melon genome. Subsequent genetic fine-mapping efforts, leveraging a larger F population encompassing 1322 plants and incorporating F phenotypic data, further refined the locus to an 80.27 kb interval housing five candidate genes. Promoter analysis and coding sequence cloning confirmed that one of these candidates, ( encoding a pentatricopeptide repeat-containing family protein, Cmppr), stands out as a strong candidate gene for the locus. Notably, comparisons of expressions across various stages of leaf development and different leaf regions suggest a pivotal role of in the morphogenesis of melon leaves.

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