Human Papillomavirus Is Rare and Does Not Correlate with P16 Expression in Non-Small-Cell Lung Cancer in a Jordanian Subpopulation

Overview

Journal Medicina (Kaunas)

Publisher MDPI

Specialty General Medicine

Date 2024 Apr 27

PMID 38674306

Authors

Ola Abu Al Karsaneh

Arwa Al Anber

Sahar AlMustafa

Hussien AlMaaitah

Batool AlQadri

Abir Igbaria

Rama Tayem

Mustafa Khasawneh

Shaima Batayha

Tareq Saleh

Mohammad Alqudah

Maher Sughayer

Affiliations

Soon will be listed here.

Abstract

: Human papillomavirus (HPV) was previously investigated in lung cancer with wide inter-geographic discrepancies. p16 has been used as a surrogate for detecting high-risk HPV (HR-HPV) in some cancer types. This study assessed the evidence of HPV in non-small-cell lung cancer (NSCLC) among Jordanian patients, investigated the expression of p16, and evaluated its prognostic value and association with HPV status. : The archived samples of 100 patients were used. HPV DNA detection was performed by real-time polymerase chain reaction (RT-PCR). p16 expression was assessed by immunohistochemistry (IHC). The Eighth American Joint Committee on Cancer protocol (AJCC) of head and neck cancer criteria were applied to evaluate p16 positivity considering a moderate/strong nuclear/cytoplasmic expression intensity with a distribution in ≥75% of cells as positive. : HPV DNA was detected in 5% of NSCLC cases. Three positive cases showed HR-HPV subtypes (16, 18, 52), and two cases showed the probable HR-HPV 26 subtype. p16 expression was positive in 20 (20%) NSCLC cases. None of the HPV-positive tumors were positive for p16 expression. A statistically significant association was identified between p16 expression and the pathological stage ( 0.029) but not with other variables. No survival impact of p16 expression was detected in NSCLC cases as a group; however, it showed a statistically significant association with overall survival (OS) in squamous cell carcinoma (SqCC) cases ( = 0.033). : This is the first study to assess HPV and p16 expression in a Jordanian population. HPV positivity is rare in NSCLC among a Jordanian subpopulation. P16 reliability as a surrogate marker for HPV infection in lung cancer must be revisited.

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