Antifungal Properties of Bio-AgNPs Against and Infection of Pea ( L.) Seedlings

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2024 Apr 27

PMID 38674112

Authors

Karolina Stalanowska

Joanna Szablinska-Piernik

Agnieszka Pszczolkowska

Viorica Railean

Milosz Wasicki

Pawel Pomastowski

Leslaw Bernard Lahuta

Adam Okorski

Affiliations

Soon will be listed here.

Abstract

Ascochyta blight and Fusarium root rot are the most serious fungal diseases of pea, caused by and , respectively. Due to the lack of fully resistant cultivars, we proposed the use of biologically synthesized silver nanoparticles (bio-AgNPs) as a novel protecting agent. In this study, we evaluated the antifungal properties and effectiveness of bio-AgNPs, in in vitro (poisoned food technique; resazurin assay) and in vivo (seedlings infection) experiments, against and . Moreover, the effects of diseases on changes in the seedlings' metabolic profiles were analyzed. The MIC for spores of both fungi was 125 mg/L, and bio-AgNPs at 200 mg/L most effectively inhibited the mycelium growth of and (by 45 and 26%, respectively, measured on the 14th day of incubation). The treatment of seedlings with bio-AgNPs or fungicides before inoculation prevented the development of infection. Bio-AgNPs at concentrations of 200 mg/L for and 100 mg/L for effectively inhibited infections' spread. The comparison of changes in polar metabolites' profiles revealed disturbances in carbon and nitrogen metabolism in pea seedlings by both pathogenic fungi. The involvement of bio-AgNPs in the mobilization of plant metabolism in response to fungal infection is also discussed.

Citing Articles

Seeds Priming with Bio-Silver Nanoparticles Protects Pea ( L.) Seedlings Against Selected Fungal Pathogens.

Stalanowska K, Railean V, Pomastowski P, Pszczolkowska A, Okorski A, Lahuta L Int J Mol Sci. 2024; 25(21).

PMID: 39518955 PMC: 11546818. DOI: 10.3390/ijms252111402.

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