TMT-Based Quantitative Proteomic Analysis Reveals the Key Role of Cell Proliferation and Apoptosis in Intestine Regeneration of

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2024 Apr 27

PMID 38673840

Authors

Chuili Zeng

Ke Xiao

Qilin Shi

Xu Zhan

Chenghua Li

Affiliations

Soon will be listed here.

Abstract

Sea cucumbers are widely known for their powerful regenerative abilities, which allow them to regenerate a complete digestive tract within a relatively short time following injury or autotomy. Recently, even though the histological changes and cellular events in the processes of intestinal regeneration have been extensively studied, the molecular machinery behind this faculty remains unclear. In this study, tandem mass tag (TMT)-based quantitation was utilized to investigate protein abundance changes during the process of intestine regeneration. Approximately 538, 445, 397, 1012, and 966 differential proteins (DEPs) were detected ( < 0.05) between the normal and 2, 7, 12, 20, and 28 dpe stages, respectively. These DEPs also mainly focus on pathways of cell proliferation and apoptosis, which were further validated by 5-Ethynyl-2'-deoxyuridine (EdU) or Tunel-based flow cytometry assay. These findings provide a reference for a comprehensive understanding of the regulatory mechanisms of various stages of intestinal regeneration and provide a foundation for subsequent research on changes in cell fate in echinoderms.

References

Garcia-Arraras J, Greenberg M . Visceral regeneration in holothurians. Microsc Res Tech. 2002; 55(6):438-51. DOI: 10.1002/jemt.1189. View

Eskandari E, Eaves C . Paradoxical roles of caspase-3 in regulating cell survival, proliferation, and tumorigenesis. J Cell Biol. 2022; 221(6). PMC: 9106709. DOI: 10.1083/jcb.202201159. View

Tseng A, Adams D, Qiu D, Koustubhan P, Levin M . Apoptosis is required during early stages of tail regeneration in Xenopus laevis. Dev Biol. 2006; 301(1):62-9. PMC: 3136124. DOI: 10.1016/j.ydbio.2006.10.048. View

Sun L, Xu D, Xu Q, Sun J, Xing L, Zhang L . iTRAQ reveals proteomic changes during intestine regeneration in the sea cucumber Apostichopus japonicus. Comp Biochem Physiol Part D Genomics Proteomics. 2017; 22:39-49. DOI: 10.1016/j.cbd.2017.02.004. View

He L, Yuan L, Yu W, Sun Y, Jiang D, Wang X . A Regulation Loop between YAP and NR4A1 Balances Cell Proliferation and Apoptosis. Cell Rep. 2020; 33(3):108284. DOI: 10.1016/j.celrep.2020.108284. View

Thompson J, Saxena S, Sharp J . Regulation of intestinal regeneration: new insights. Microsc Res Tech. 2000; 51(2):129-37. DOI: 10.1002/1097-0029(20001015)51:2<129::AID-JEMT4>3.0.CO;2-Y. View

Garcia-Arraras J, Santiago R, Torres I, Diaz-Miranda L . Cellular mechanisms of intestine regeneration in the sea cucumber, Holothuria glaberrima Selenka (Holothuroidea:Echinodermata). J Exp Zool. 1998; 281(4):288-304. DOI: 10.1002/(sici)1097-010x(19980701)281:4<288::aid-jez5>3.0.co;2-k. View

Lv Z, Guo M, Li C, Shao Y, Zhao X, Zhang W . Divergent proteomics response of Apostichopus japonicus suffering from skin ulceration syndrome and pathogen infection. Comp Biochem Physiol Part D Genomics Proteomics. 2019; 30:196-205. DOI: 10.1016/j.cbd.2019.03.003. View

Quispe-Parra D, Valentin G, Garcia-Arraras J . A roadmap for intestinal regeneration. Int J Dev Biol. 2020; 65(4-5-6):427-437. PMC: 9503165. DOI: 10.1387/ijdb.200227dq. View

10.

Sehring I, Jahn C, Weidinger G . Zebrafish fin and heart: what's special about regeneration?. Curr Opin Genet Dev. 2016; 40:48-56. DOI: 10.1016/j.gde.2016.05.011. View

11.

Ivankovic M, Haneckova R, Thommen A, Grohme M, Vila-Farre M, Werner S . Model systems for regeneration: planarians. Development. 2019; 146(17). DOI: 10.1242/dev.167684. View

12.

Huang Z, Chen C, Zeng Z, Yang H, Oh J, Chen L . Mechanism and significance of increased glutathione level in human hepatocellular carcinoma and liver regeneration. FASEB J. 2000; 15(1):19-21. DOI: 10.1096/fj.00-0445fje. View

13.

Mashanov V, Zueva O, Garcia-Arraras J . Expression of pluripotency factors in echinoderm regeneration. Cell Tissue Res. 2014; 359(2):521-536. PMC: 4323854. DOI: 10.1007/s00441-014-2040-4. View

14.

Bijnens K, Jaenen V, Wouters A, Leynen N, Pirotte N, Artois T . A Spatiotemporal Characterisation of Redox Molecules in Planarians, with a Focus on the Role of Glutathione during Regeneration. Biomolecules. 2021; 11(5). PMC: 8150688. DOI: 10.3390/biom11050714. View

15.

Yuan J, Gao Y, Sun L, Jin S, Zhang X, Liu C . Wnt Signaling Pathway Linked to Intestinal Regeneration via Evolutionary Patterns and Gene Expression in the Sea Cucumber . Front Genet. 2019; 10:112. PMC: 6390002. DOI: 10.3389/fgene.2019.00112. View

16.

Zeng C, Guo M, Xiang Y, Song M, Xiao K, Li C . Mesentery AjFGF4-AjFGFR2-ERK pathway modulates intestinal regeneration via targeting cell cycle in echinoderms. Cell Prolif. 2022; 56(2):e13351. PMC: 9890533. DOI: 10.1111/cpr.13351. View

17.

Gurtner G, Werner S, Barrandon Y, Longaker M . Wound repair and regeneration. Nature. 2008; 453(7193):314-21. DOI: 10.1038/nature07039. View

18.

Kashio S, Obata F, Miura M . Interplay of cell proliferation and cell death in Drosophila tissue regeneration. Dev Growth Differ. 2014; 56(5):368-75. DOI: 10.1111/dgd.12139. View

19.

Ricci L, Srivastava M . Wound-induced cell proliferation during animal regeneration. Wiley Interdiscip Rev Dev Biol. 2018; 7(5):e321. DOI: 10.1002/wdev.321. View

20.

Barter R, Yu B . Superheat: An R package for creating beautiful and extendable heatmaps for visualizing complex data. J Comput Graph Stat. 2019; 27(4):910-922. PMC: 6430237. DOI: 10.1080/10618600.2018.1473780. View