The MYB Transcription Factor Negatively Regulates Resistance in Soybean

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2024 Apr 27

PMID 38673832

Authors

Hong Gao

Jia Ma

Yuxin Zhao

Chuanzhong Zhang

Ming Zhao

Shengfu He

Yan Sun

Xin Fang

Xiaoyu Chen

Kexin Ma

Yanjie Pang

Yachang Gu

Yaqun Dongye

Junjiang Wu

Pengfei Xu

Shuzhen Zhang

Affiliations

Soon will be listed here.

Abstract

Phytophthora root rot is a devastating disease of soybean caused by . However, the resistance mechanism is not yet clear. Our previous studies have shown that enhances sensitivity to in soybean, and is downregulated in the transcriptome analysis of -overexpressing transgenic hairy roots. Here, was significantly induced by in susceptible soybean, and the overexpressing of enhanced sensitivity to the pathogen, while silencing enhances resistance to , indicating that is a negative regulator of . Moreover, the jasmonic acid (JA) content and JA synthesis gene was highly upregulated in -silencing roots and highly downregulated in overexpressing ones, suggesting that could respond to through the JA signaling pathway. Furthermore, the expression of several pathogenesis-related genes was significantly lower in -overexpressing roots and higher in -silencing ones. Additionally, we screened and identified the upstream regulator GmbHLH122 and downstream target gene of GmMYB78. GmbHLH122 was highly induced by and could inhibit expression in resistant soybean, and GmMYB78 was highly expressed to activate downstream target gene transcription in susceptible soybean. In conclusion, our data reveal that triggers soybean sensitivity to by inhibiting the JA signaling pathway and the expression of pathogenesis-related genes or through the effects of the GmbHLH122-GmMYB78- cascade pathway.

Citing Articles

Root rot in medicinal plants: a review of extensive research progress.

Han Y, Sun T, Tang Y, Yang M, Gao W, Wang L Front Plant Sci. 2025; 15:1504370.

PMID: 39963361 PMC: 11830675. DOI: 10.3389/fpls.2024.1504370.

References

Longsaward R, Pengnoo A, Kongsawadworakul P, Viboonjun U . A novel rubber tree PR-10 protein involved in host-defense response against the white root rot fungus Rigidoporus microporus. BMC Plant Biol. 2023; 23(1):157. PMC: 10032002. DOI: 10.1186/s12870-023-04149-3. View

Shen X, Wang Y, Zhang Y, Guo W, Jiao Y, Zhou X . Overexpression of the Wild Soybean R2R3-MYB Transcription Factor Enhances Resistance to Salt Stress and in Transgenic . Int J Mol Sci. 2018; 19(12). PMC: 6321161. DOI: 10.3390/ijms19123958. View

Song M, Fang S, Li Z, Wang N, Li X, Liu W . CsAtf1, a bZIP transcription factor, is involved in fludioxonil sensitivity and virulence in the rubber tree anthracnose fungus Colletotrichum siamense. Fungal Genet Biol. 2021; 158:103649. DOI: 10.1016/j.fgb.2021.103649. View

Zhang Y, Fan W, Kinkema M, Li X, Dong X . Interaction of NPR1 with basic leucine zipper protein transcription factors that bind sequences required for salicylic acid induction of the PR-1 gene. Proc Natl Acad Sci U S A. 1999; 96(11):6523-8. PMC: 26915. DOI: 10.1073/pnas.96.11.6523. View

WARD E, Uknes S, Williams S, Dincher S, Wiederhold D, Alexander D . Coordinate Gene Activity in Response to Agents That Induce Systemic Acquired Resistance. Plant Cell. 1991; 3(10):1085-1094. PMC: 160074. DOI: 10.1105/tpc.3.10.1085. View

Zhang S, Zhao Q, Zeng D, Xu J, Zhou H, Wang F . RhMYB108, an R2R3-MYB transcription factor, is involved in ethylene- and JA-induced petal senescence in rose plants. Hortic Res. 2019; 6:131. PMC: 6885062. DOI: 10.1038/s41438-019-0221-8. View

Han Z, Zhang C, Zhang H, Duan Y, Zou Z, Zhou L . CsMYB Transcription Factors Participate in Jasmonic Acid Signal Transduction in Response to Cold Stress in Tea Plant (). Plants (Basel). 2022; 11(21). PMC: 9654479. DOI: 10.3390/plants11212869. View

Sugimoto K, Takeda S, Hirochika H . MYB-related transcription factor NtMYB2 induced by wounding and elicitors is a regulator of the tobacco retrotransposon Tto1 and defense-related genes. Plant Cell. 2001; 12(12):2511-2528. PMC: 102234. DOI: 10.1105/tpc.12.12.2511. View

Liu S, Gao X, Shi M, Sun M, Li K, Cai Y . Jasmonic acid regulates the biosynthesis of medicinal metabolites via the JAZ9-MYB76 complex in . Hortic Res. 2023; 10(3):uhad004. PMC: 10022484. DOI: 10.1093/hr/uhad004. View

10.

Cheng Q, Dong L, Gao T, Liu T, Li N, Wang L . The bHLH transcription factor GmPIB1 facilitates resistance to Phytophthora sojae in Glycine max. J Exp Bot. 2018; 69(10):2527-2541. PMC: 5920285. DOI: 10.1093/jxb/ery103. View

11.

Xiang Q, Judelson H . Myb transcription factors in the oomycete Phytophthora with novel diversified DNA-binding domains and developmental stage-specific expression. Gene. 2010; 453(1-2):1-8. DOI: 10.1016/j.gene.2009.12.006. View

12.

Hong Y, Liu Q, Cao Y, Zhang Y, Chen D, Lou X . The Negatively Regulates and Disease Resistance via SA and JA Signaling Pathway in Rice. Front Plant Sci. 2019; 10:752. PMC: 6598650. DOI: 10.3389/fpls.2019.00752. View

13.

Tyler B . Phytophthora sojae: root rot pathogen of soybean and model oomycete. Mol Plant Pathol. 2010; 8(1):1-8. DOI: 10.1111/j.1364-3703.2006.00373.x. View

14.

Qiu B, Chen H, Zheng L, Su L, Cui X, Ge F . An MYB Transcription Factor Modulates Resistance Against the Root Rot Pathogen by Regulating the Jasmonate Acid Signaling Pathway and Photosynthesis. Phytopathology. 2021; 112(6):1323-1334. DOI: 10.1094/PHYTO-07-21-0283-R. View

15.

Bilaud T, Koering C, Ancelin K, Pollice A, Gasser S, Gilson E . The telobox, a Myb-related telomeric DNA binding motif found in proteins from yeast, plants and human. Nucleic Acids Res. 1996; 24(7):1294-303. PMC: 145771. DOI: 10.1093/nar/24.7.1294. View

16.

Zhang C, Gao H, Li R, Han D, Wang L, Wu J . GmBTB/POZ, a novel BTB/POZ domain-containing nuclear protein, positively regulates the response of soybean to Phytophthora sojae infection. Mol Plant Pathol. 2018; 20(1):78-91. PMC: 6430474. DOI: 10.1111/mpp.12741. View

17.

Dutta S, Kumawat G, Singh B, Gupta D, Singh S, Dogra V . Development of genic-SSR markers by deep transcriptome sequencing in pigeonpea [Cajanus cajan (L.) Millspaugh]. BMC Plant Biol. 2011; 11:17. PMC: 3036606. DOI: 10.1186/1471-2229-11-17. View

18.

Wang F, Lin R, Li Y, Wang P, Feng J, Chen W . TabZIP74 Acts as a Positive Regulator in Wheat Stripe Rust Resistance and Involves Root Development by mRNA Splicing. Front Plant Sci. 2020; 10:1551. PMC: 6927285. DOI: 10.3389/fpls.2019.01551. View

19.

Gu K, Zhang Q, Yu J, Wang J, Zhang F, Wang C . R2R3-MYB Transcription Factor MdMYB73 Confers Increased Resistance to the Fungal Pathogen in Apples the Salicylic Acid Pathway. J Agric Food Chem. 2020; 69(1):447-458. DOI: 10.1021/acs.jafc.0c06740. View

20.

Noman A, Hussain A, Adnan M, Khan M, Ashraf M, Zainab M . A novel MYB transcription factor CaPHL8 provide clues about evolution of pepper immunity againstsoil borne pathogen. Microb Pathog. 2019; 137:103758. DOI: 10.1016/j.micpath.2019.103758. View