Effect of Low Temperature on Content of Primary Metabolites in Two Wheat Genotypes Differing in Cold Tolerance

Overview

Journal Metabolites

Publisher MDPI

Date 2024 Apr 26

PMID 38668327

Authors

Alexander Deryabin

Kseniya Zhukova

Natalia Naraikina

Yuliya Venzhik

Affiliations

Soon will be listed here.

Abstract

The study of cold-tolerance mechanisms of wheat as a leading cereal crop is very relevant to science. Primary metabolites play an important role in the formation of increased cold tolerance. The aim of this research is to define changes in the content of primary metabolites (soluble proteins and sugars), growth, and photosynthetic apparatus of freezing-tolerant and cold-sustainable wheat ( L.) genotypes under optimal conditions and after prolonged (7 days) exposure to low temperature (4 °C). In order to gain a deeper comprehension of the mechanisms behind wheat genotypes' adaptation to cold, we determined the expression levels of photosynthetic genes (, ) and genes encoding cold-regulated proteins (, ). The results indicated different cold-adaptation strategies of freezing-tolerant and cold-sustainable wheat genotypes, with soluble proteins and sugars playing a significant role in this process. In plants of freezing-tolerant genotypes, the strategy of adaptation to low temperature was aimed at increasing the content of soluble proteins and modification of carbohydrate metabolism. The accumulation of sugars was not observed in wheat of cold-sustainable genotypes during chilling, but a high content of soluble proteins was maintained both under optimal conditions and after cold exposure. The adaptation strategies of wheat genotypes differing in cold tolerance were related to the expression of photosynthetic genes and genes encoding cold-regulated proteins. The data improve our knowledge of physiological and biochemical mechanisms of wheat cold adaptation.

References

Matros A, Peshev D, Peukert M, Mock H, Van den Ende W . Sugars as hydroxyl radical scavengers: proof-of-concept by studying the fate of sucralose in Arabidopsis. Plant J. 2015; 82(5):822-39. DOI: 10.1111/tpj.12853. View

Fernandez O, Theocharis A, Bordiec S, Feil R, Jacquens L, Clement C . Burkholderia phytofirmans PsJN acclimates grapevine to cold by modulating carbohydrate metabolism. Mol Plant Microbe Interact. 2012; 25(4):496-504. DOI: 10.1094/MPMI-09-11-0245. View

Bies-Etheve N, Gaubier-Comella P, Debures A, Lasserre E, Jobet E, Raynal M . Inventory, evolution and expression profiling diversity of the LEA (late embryogenesis abundant) protein gene family in Arabidopsis thaliana. Plant Mol Biol. 2008; 67(1-2):107-24. DOI: 10.1007/s11103-008-9304-x. View

Kaplan F, Kopka J, Sung D, Zhao W, Popp M, Porat R . Transcript and metabolite profiling during cold acclimation of Arabidopsis reveals an intricate relationship of cold-regulated gene expression with modifications in metabolite content. Plant J. 2007; 50(6):967-81. DOI: 10.1111/j.1365-313X.2007.03100.x. View

Perdomo J, Buchner P, Carmo-Silva E . The relative abundance of wheat Rubisco activase isoforms is post-transcriptionally regulated. Photosynth Res. 2021; 148(1-2):47-56. PMC: 8154801. DOI: 10.1007/s11120-021-00830-6. View

Tjus S, Scheller H, Andersson B, Moller B . Active oxygen produced during selective excitation of photosystem I is damaging not only to photosystem I, but also to photosystem II. Plant Physiol. 2001; 125(4):2007-15. PMC: 88856. DOI: 10.1104/pp.125.4.2007. View

Zhang B, Jia D, Gao Z, Dong Q, He L . Physiological responses to low temperature in spring and winter wheat varieties. J Sci Food Agric. 2015; 96(6):1967-73. DOI: 10.1002/jsfa.7306. View

Shewry P . Wheat. J Exp Bot. 2009; 60(6):1537-53. DOI: 10.1093/jxb/erp058. View

Frederiks T, Christopher J, Sutherland M, Borrell A . Post-head-emergence frost in wheat and barley: defining the problem, assessing the damage, and identifying resistance. J Exp Bot. 2015; 66(12):3487-98. DOI: 10.1093/jxb/erv088. View

10.

Sum A, Faller R, de Pablo J . Molecular simulation study of phospholipid bilayers and insights of the interactions with disaccharides. Biophys J. 2003; 85(5):2830-44. PMC: 1303564. DOI: 10.1016/s0006-3495(03)74706-7. View

11.

Pashkovskiy P, Kreslavski V, Ivanov Y, Ivanova A, Kartashov A, Shmarev A . Influence of Light of Different Spectral Compositions on the Growth, Photosynthesis, and Expression of Light-Dependent Genes of Scots Pine Seedlings. Cells. 2021; 10(12). PMC: 8699472. DOI: 10.3390/cells10123284. View

12.

Xu J, Li Y, Sun J, Du L, Zhang Y, Yu Q . Comparative physiological and proteomic response to abrupt low temperature stress between two winter wheat cultivars differing in low temperature tolerance. Plant Biol (Stuttg). 2012; 15(2):292-303. DOI: 10.1111/j.1438-8677.2012.00639.x. View

13.

Kamata T, Uemura M . Solute accumulation in heat seedlings during cold acclimation: contribution to increased freezing tolerance. Cryo Letters. 2004; 25(5):311-22. View

14.

Knox A, Li C, Vagujfalvi A, Galiba G, Stockinger E, Dubcovsky J . Identification of candidate CBF genes for the frost tolerance locus Fr-Am2 in Triticum monococcum. Plant Mol Biol. 2008; 67(3):257-70. DOI: 10.1007/s11103-008-9316-6. View

15.

Du Fall L, Solomon P . Role of cereal secondary metabolites involved in mediating the outcome of plant-pathogen interactions. Metabolites. 2014; 1(1):64-78. PMC: 4012515. DOI: 10.3390/metabo1010064. View

16.

Reyes-Diaz M, Ulloa N, Zuniga-Feest A, Gutierrez A, Gidekel M, Alberdi M . Arabidopsis thaliana avoids freezing by supercooling. J Exp Bot. 2006; 57(14):3687-96. DOI: 10.1093/jxb/erl125. View

17.

Xue G, Sadat S, Drenth J, McIntyre C . The heat shock factor family from Triticum aestivum in response to heat and other major abiotic stresses and their role in regulation of heat shock protein genes. J Exp Bot. 2013; 65(2):539-57. PMC: 3904712. DOI: 10.1093/jxb/ert399. View

18.

Sami F, Yusuf M, Faizan M, Faraz A, Hayat S . Role of sugars under abiotic stress. Plant Physiol Biochem. 2016; 109:54-61. DOI: 10.1016/j.plaphy.2016.09.005. View

19.

Liu Q, Zhang X, Su Y, Zhang X . Genetic Mechanisms of Cold Signaling in Wheat ( L.). Life (Basel). 2022; 12(5). PMC: 9147279. DOI: 10.3390/life12050700. View

20.

Sarhadi E, Mahfoozi S, Hosseini S, Hosseini Salekdeh G . Cold acclimation proteome analysis reveals close link between the up-regulation of low-temperature associated proteins and vernalization fulfillment. J Proteome Res. 2010; 9(11):5658-67. DOI: 10.1021/pr100475r. View